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Published in:
2018 | OriginalPaper | Chapter
Evaluation of Depth Perturbation Method for Localizing Fluorophore Inside a Pork Chuck Meat Sample
Authors : Ichiro Sakuma, Tuo Zhou, Keiichi Nakagawa, Etsuko Kobayashi
Published in: 2nd International Conference for Innovation in Biomedical Engineering and Life Sciences
Publisher: Springer Singapore
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Abstract
Depth perturbation method for improvement of a mesoscopic epifluorescence tomography (MEFT) we previously developed was evaluated in an experimental system using biological samples. We conducted ex vivo experiments using pork chuck meat to verify the depth perturbation concept in a relatively realistic environment. Fluorescence images were acquired with/without a thin optical phantom (perturbator with known optical scattering property) onto the phantom. Fluorescence intensity variation resulting from the depth perturbation was used for estimating three dimensional location of fluorophores. Estimated fluorophore central depths (placed at depth from 3–9 mm) are consistent with the expected values. Absolute estimation errors of the center position of fluorophores placed inside the phantom were 0.3–0.5 mm (on average). Slight optical heterogeneity did not bring great estimating errors.