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Published in: Adsorption 3/2018

05-03-2018

Expanded bed adsorption of albumin and immunoglobulin G from human serum onto a cation exchanger mixed mode adsorbent

Authors: Pedro Ferreira Gomes, José Miguel Loureiro, Alírio E. Rodrigues

Published in: Adsorption | Issue 3/2018

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Abstract

Adsorption of human serum albumin (HSA) and immunoglobulin G (IgG), two most relevant proteins in blood serum were measured separately on a new mixed-mode adsorbent (MabDirect MM), especially designed for expanded bed adsorption process. Expanded bed behavior characterization by residence time distribution experiments for different columns (Streamline 50 for HSA study, Omnifit 66/20 and XK 16/20 for IgG study) are presented together with breakthrough adsorption of HSA and IgG for different conditions and compared with simulation results by a mathematical model. Regarding HSA adsorption, all four experiments were conducted at the same buffer solution (pH 5.0 without salt), and it was obtained a dynamic binding capacity of 8.9, 9.7, 7.5 and 7.0 mg·gdry−1 (24.8, 27.0, 21.0 and 19.5 mg·cm−3) at 10% of breakthrough, corresponding to a 41, 39, 38 and 30% of the saturation capacity for runs 1–4, respectively. The results from the simulation of the mathematical model fitted well with the breakthrough experiments. Elution stage was optimized by lowering flow rate and changing the buffer solution pH and NaCl concentration. Concerning IgG adsorption, for IgG feed concentration of 0.53 g·dm−3 in 20 mM citrate buffer, pH 5.0 with 0.4 M NaCl, at 2.2 cm3·min−1, it was obtained a DBC of 3.3 mg·gdry−1 (9.1 mg·cm−3) at 10% of breakthrough, representing 22% of the saturation capacity. While for a feed concentration of 0.11 g·dm−3 of IgG in the same buffer solution, at 6 cm3·min−1, it was obtained a DBC of 2.7 mg·gdry−1 (7.4 mg·cm−3) at 10% of breakthrough, representing 34% of the saturation capacity.

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Appendix
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Literature
go back to reference Antibody Production and Purification Technical Handbook. Thermo Scientific, Waltham (2010) Antibody Production and Purification Technical Handbook. Thermo Scientific, Waltham (2010)
go back to reference Arévalo, E., Rendueles, M., Fernández, A., Díaz, M.: Equilibrium and simulation of the operation for the adsorption of albumin proteins in an iminodiacetic-Cu bounded ion exchange resin (IMAC). Sep. Purif. Technol. 18, 217–225 (2000)CrossRef Arévalo, E., Rendueles, M., Fernández, A., Díaz, M.: Equilibrium and simulation of the operation for the adsorption of albumin proteins in an iminodiacetic-Cu bounded ion exchange resin (IMAC). Sep. Purif. Technol. 18, 217–225 (2000)CrossRef
go back to reference Bruce, L.J., Chase, H.A.: Hydrodynamics and adsorption behaviour within an expanded bed adsorption column studied using in-bed sampling. Chem. Eng. Sci. 56, 3149–3162 (2001)CrossRef Bruce, L.J., Chase, H.A.: Hydrodynamics and adsorption behaviour within an expanded bed adsorption column studied using in-bed sampling. Chem. Eng. Sci. 56, 3149–3162 (2001)CrossRef
go back to reference Carson, K.L.: Flexibility—the guiding principle for antibody manufacturing. Nat Biotechnol. 23, 1054–1058 (2005)CrossRef Carson, K.L.: Flexibility—the guiding principle for antibody manufacturing. Nat Biotechnol. 23, 1054–1058 (2005)CrossRef
go back to reference Chase, H.A., Draeger, N.M.: Affinity purification of proteins using expanded beds. J. Chromatogr. A 597, 129–145 (1992)CrossRef Chase, H.A., Draeger, N.M.: Affinity purification of proteins using expanded beds. J. Chromatogr. A 597, 129–145 (1992)CrossRef
go back to reference de Andrade Lima, M., de Freitas, M.D., Gonçalves, L.R., da Silva Junior, I.J.: Recovery and purification of a Kluyvermyces lactis β-galactosidase by mixed mode chromatography. J. Chromatogr. B 1015–1016, 181–191 (2016)CrossRef de Andrade Lima, M., de Freitas, M.D., Gonçalves, L.R., da Silva Junior, I.J.: Recovery and purification of a Kluyvermyces lactis β-galactosidase by mixed mode chromatography. J. Chromatogr. B 1015–1016, 181–191 (2016)CrossRef
go back to reference Du, Q.Y., Lin, D.Q., Zhang, Q.L., Yao, S.J.: An integrated expanded bed adsorption process for lactoferrin and immunoglobulin G purification from crude sweet whey. J Chromatogr B 947–948, 201–207 (2014)CrossRef Du, Q.Y., Lin, D.Q., Zhang, Q.L., Yao, S.J.: An integrated expanded bed adsorption process for lactoferrin and immunoglobulin G purification from crude sweet whey. J Chromatogr B 947–948, 201–207 (2014)CrossRef
go back to reference Fan, L.-T., Yang, Y.-C., Wen, C.-Y.: Mass transfer in semifluidized beds for solid-liquid system. AIChE J. 6, 482–487 (1960)CrossRef Fan, L.-T., Yang, Y.-C., Wen, C.-Y.: Mass transfer in semifluidized beds for solid-liquid system. AIChE J. 6, 482–487 (1960)CrossRef
go back to reference Fanali, G., di Masi, A., Trezza, V., Marino, M., Fasano, M., Ascenzi, P.: Human serum albumin: from bench to bedside molecular. Asp. Med. 33, 209–290 (2012)CrossRef Fanali, G., di Masi, A., Trezza, V., Marino, M., Fasano, M., Ascenzi, P.: Human serum albumin: from bench to bedside molecular. Asp. Med. 33, 209–290 (2012)CrossRef
go back to reference Feuser, J., Halfar, M., Lutkemeyer, D., Ameskamp, N., Kula, M., Thommes, J.: Interaction of mammalian cell culture broth with adsorbents in expanded bed adsorption of monoclonal antibodies. Process Biochem. 34, 159–165 (1999)CrossRef Feuser, J., Halfar, M., Lutkemeyer, D., Ameskamp, N., Kula, M., Thommes, J.: Interaction of mammalian cell culture broth with adsorbents in expanded bed adsorption of monoclonal antibodies. Process Biochem. 34, 159–165 (1999)CrossRef
go back to reference Gomes, P.F., Loureiro, J.M., Rodrigues, A.E.: Adsorption equilibrium and kinetics of Immunoglobulin G on a mixed-mode adsorbent in batch and packed bed configuration. J. Chromatogr. A 1524, 143–152 (2017a)CrossRef Gomes, P.F., Loureiro, J.M., Rodrigues, A.E.: Adsorption equilibrium and kinetics of Immunoglobulin G on a mixed-mode adsorbent in batch and packed bed configuration. J. Chromatogr. A 1524, 143–152 (2017a)CrossRef
go back to reference Gomes, P.F., Loureiro, J.M., Rodrigues, A.E.: Adsorption of Human Serum Albumin (HSA) on a mixed-mode adsorbent: equilibrium and kinetics Adsorption 23, 491–505 (2017b)CrossRef Gomes, P.F., Loureiro, J.M., Rodrigues, A.E.: Adsorption of Human Serum Albumin (HSA) on a mixed-mode adsorbent: equilibrium and kinetics Adsorption 23, 491–505 (2017b)CrossRef
go back to reference Hashim, M.A., Chu, K.H., Tsan, P.S.: Modelling of protein adsorption in a fixed-bed: Singule and Two-soute breakthrough behaviour. Chem. Eng. Commun. 161, 45–63 (1997)CrossRef Hashim, M.A., Chu, K.H., Tsan, P.S.: Modelling of protein adsorption in a fixed-bed: Singule and Two-soute breakthrough behaviour. Chem. Eng. Commun. 161, 45–63 (1997)CrossRef
go back to reference Hoffman, W.L., O’Shannessy, D.J.: Site-specific immobilization of antibodies by their oligosaccharide moieties to new hydrazide derivatized solid supports. J. Immunol. Methods 112, 113–120 (1988)CrossRef Hoffman, W.L., O’Shannessy, D.J.: Site-specific immobilization of antibodies by their oligosaccharide moieties to new hydrazide derivatized solid supports. J. Immunol. Methods 112, 113–120 (1988)CrossRef
go back to reference Kelly, W., et al.: Experimental characterization of next-generation expanded-bed adsorbents for capture of a recombinant protein expressed in high-cell-density yeast fermentation. Biotechnol. Appl. Biochem. 60, 510–520 (2013a)CrossRef Kelly, W., et al.: Experimental characterization of next-generation expanded-bed adsorbents for capture of a recombinant protein expressed in high-cell-density yeast fermentation. Biotechnol. Appl. Biochem. 60, 510–520 (2013a)CrossRef
go back to reference Kelly, W., Kamguia, G., Mullen, P., Ubiera, A., Göklen, K., Huang, Z., Jones, G.: Using a two species competitive binding model to predict expanded bed breakthrough of a recombinant protein expressed in a high cell density fermentation. Biotechnol. Bioprocess Eng. 18, 546–559 (2013b)CrossRef Kelly, W., Kamguia, G., Mullen, P., Ubiera, A., Göklen, K., Huang, Z., Jones, G.: Using a two species competitive binding model to predict expanded bed breakthrough of a recombinant protein expressed in a high cell density fermentation. Biotechnol. Bioprocess Eng. 18, 546–559 (2013b)CrossRef
go back to reference Li, P.: Protein separation and purification by expanded bed chromatography and simulated moving bed technology. Faculty of Engineering University of Porto (2006) Li, P.: Protein separation and purification by expanded bed chromatography and simulated moving bed technology. Faculty of Engineering University of Porto (2006)
go back to reference Li, P., Xiu, G., Rodrigues, A.E.: Modeling separation of proteins by inert core adsorbent in a batch adsorber. Chem. Eng. Sci. 58, 3361–3371 (2003)CrossRef Li, P., Xiu, G., Rodrigues, A.E.: Modeling separation of proteins by inert core adsorbent in a batch adsorber. Chem. Eng. Sci. 58, 3361–3371 (2003)CrossRef
go back to reference Li, P., Xiu, G., Rodrigues, A.E.: Experimental and modeling study of protein adsorption in expanded bed. Am. Inst. Chem. Eng. J. 51, 2965–2977 (2005)CrossRef Li, P., Xiu, G., Rodrigues, A.E.: Experimental and modeling study of protein adsorption in expanded bed. Am. Inst. Chem. Eng. J. 51, 2965–2977 (2005)CrossRef
go back to reference Li, P., Gomes, P.F., Loureiro, J.M., Rodrigues, A.E.: Proteins separation and purification by expanded bed adsorption and simulated moving bed technology. In: Subramanian, G. (ed.) Continuous Processing in Pharmaceutical Manufacturing. Wiley, Weinheim (2014) Li, P., Gomes, P.F., Loureiro, J.M., Rodrigues, A.E.: Proteins separation and purification by expanded bed adsorption and simulated moving bed technology. In: Subramanian, G. (ed.) Continuous Processing in Pharmaceutical Manufacturing. Wiley, Weinheim (2014)
go back to reference Malm, B.: A method suitable for the isolation of monoclonal antibodies from large volumes of serum-containing hybridoma cell culture supernatants. J. Immunol. Methods. 104, 103–109 (1987)CrossRef Malm, B.: A method suitable for the isolation of monoclonal antibodies from large volumes of serum-containing hybridoma cell culture supernatants. J. Immunol. Methods. 104, 103–109 (1987)CrossRef
go back to reference Martin, C., Iberer, G., Ubiera, A., Carta, G.: Two-component protein adsorption kinetics in porous ion exchange media. J. Chromatogr. A 1079, 105–115 (2005)CrossRef Martin, C., Iberer, G., Ubiera, A., Carta, G.: Two-component protein adsorption kinetics in porous ion exchange media. J. Chromatogr. A 1079, 105–115 (2005)CrossRef
go back to reference Palsson, E., Axelsson, A., Larsson, P.O.: Theories of chromatographic efficiency applied to expanded beds. J. Chromatogr. A 912, 235–248 (2001)CrossRef Palsson, E., Axelsson, A., Larsson, P.O.: Theories of chromatographic efficiency applied to expanded beds. J. Chromatogr. A 912, 235–248 (2001)CrossRef
go back to reference Ranjini, S.S., Bimal, D., Dhivya, A.P., Vijayalakshmi, M.A.: Study of the mechanism of interaction of antibody (IgG) on two mixed mode sorbents. J. Chromatogr. B 878, 1031–1037 (2010)CrossRef Ranjini, S.S., Bimal, D., Dhivya, A.P., Vijayalakshmi, M.A.: Study of the mechanism of interaction of antibody (IgG) on two mixed mode sorbents. J. Chromatogr. B 878, 1031–1037 (2010)CrossRef
go back to reference Ranz, W., Marshall, W.: Evaporation from drops. Chem. Eng. Prog. 48, 141–146 (1952) Ranz, W., Marshall, W.: Evaporation from drops. Chem. Eng. Prog. 48, 141–146 (1952)
go back to reference Rendueles de la Vega M., Chenou, C., Loureiro, J.M., Rodrigues, A.E.: Mass transfer mechanisms in Hyper D media for chromatographic protein separation. Biochem. Eng. J. 1, 11–23 (1998)CrossRef Rendueles de la Vega M., Chenou, C., Loureiro, J.M., Rodrigues, A.E.: Mass transfer mechanisms in Hyper D media for chromatographic protein separation. Biochem. Eng. J. 1, 11–23 (1998)CrossRef
go back to reference Rezvani, A., Jahanshahi, M., Najafpour, G.D.: Characterization and evaluation of the novel agarose–nickel composite matrix for possible use in expanded bed adsorption of bio-products. J. Chromatogr. A 1331, 61–68 (2014)CrossRef Rezvani, A., Jahanshahi, M., Najafpour, G.D.: Characterization and evaluation of the novel agarose–nickel composite matrix for possible use in expanded bed adsorption of bio-products. J. Chromatogr. A 1331, 61–68 (2014)CrossRef
go back to reference Schaller, J., Gerber, S., Kaempfer, U., Lejon, S., Trachsel, C.: Human Blood Plasma Proteins: Structure and Function. Wiley, Chichester (2008)CrossRef Schaller, J., Gerber, S., Kaempfer, U., Lejon, S., Trachsel, C.: Human Blood Plasma Proteins: Structure and Function. Wiley, Chichester (2008)CrossRef
go back to reference Shi, W., Lin, D.-Q., Tong, H.-F., Yun, J.-X., Yao, S.-J.: 5-Aminobenzimidazole as new hydrophobic charge-induction ligand for expanded bed adsorption of bovine IgG. J. Chromatogr. A 1425, 97–105 (2015)CrossRef Shi, W., Lin, D.-Q., Tong, H.-F., Yun, J.-X., Yao, S.-J.: 5-Aminobenzimidazole as new hydrophobic charge-induction ligand for expanded bed adsorption of bovine IgG. J. Chromatogr. A 1425, 97–105 (2015)CrossRef
go back to reference Skidmore, G.L., Chase, H.A.: Two-component protein adsorption to the cation exchanger S Sepharose® FF. J. Chromatogr. A 505, 329–347 (1990)CrossRef Skidmore, G.L., Chase, H.A.: Two-component protein adsorption to the cation exchanger S Sepharose® FF. J. Chromatogr. A 505, 329–347 (1990)CrossRef
go back to reference Thommes, J., Halfar, M., Lenz, S., Kula, M.R.: Purification of monoclonal antibodies from whole hybridoma fermentation broth by fluidized bed adsorption. Biotechnol. Bioeng. 45, 205–211 (1995)CrossRef Thommes, J., Halfar, M., Lenz, S., Kula, M.R.: Purification of monoclonal antibodies from whole hybridoma fermentation broth by fluidized bed adsorption. Biotechnol. Bioeng. 45, 205–211 (1995)CrossRef
go back to reference Thommes, J., Bader, A., Halfar, M., Karau, A., Kula, M.R.: Isolation of monoclonal antibodies from cell containing hybridoma broth using a protein A coated adsorbent in expanded beds. J. Chromatogr. A 752, 111–122 (1996)CrossRef Thommes, J., Bader, A., Halfar, M., Karau, A., Kula, M.R.: Isolation of monoclonal antibodies from cell containing hybridoma broth using a protein A coated adsorbent in expanded beds. J. Chromatogr. A 752, 111–122 (1996)CrossRef
go back to reference Van Der Meer, A.P., Blanchard, C.M.R.J.P., Wesselingh, J.A.: Mixing of particles in liquid fluidized beds. Chem. Eng. Res. Des. 62, 214–222 (1984) Van Der Meer, A.P., Blanchard, C.M.R.J.P., Wesselingh, J.A.: Mixing of particles in liquid fluidized beds. Chem. Eng. Res. Des. 62, 214–222 (1984)
go back to reference Wakao, N., Funazkri, T.: Effect of fluid dispersion coefficients on particle-to-fluid mass transfer coefficients in packed beds: correlation of sherwood numbers. Chem. Eng. Sci. 33, 1375–1384 (1978)CrossRef Wakao, N., Funazkri, T.: Effect of fluid dispersion coefficients on particle-to-fluid mass transfer coefficients in packed beds: correlation of sherwood numbers. Chem. Eng. Sci. 33, 1375–1384 (1978)CrossRef
go back to reference Walters, R.R., Graham, J.F., Moore, R.M., Anderson, D.J.: Protein diffusion coefficient measurements by laminar flow analysis: method and applications. Anal. Biochem. 140, 190–195 (1984)CrossRef Walters, R.R., Graham, J.F., Moore, R.M., Anderson, D.J.: Protein diffusion coefficient measurements by laminar flow analysis: method and applications. Anal. Biochem. 140, 190–195 (1984)CrossRef
go back to reference Weinbrenner, W.F., Etzel, M.R.: Competitive adsorption of alpha-lactalbumin and bovine serum albumin to a sulfopropyl ion-exchange membrane. J. Chromatogr. A 662, 414–419 (1994)CrossRef Weinbrenner, W.F., Etzel, M.R.: Competitive adsorption of alpha-lactalbumin and bovine serum albumin to a sulfopropyl ion-exchange membrane. J. Chromatogr. A 662, 414–419 (1994)CrossRef
go back to reference Wilson, E.J., Geankoplis, C.J.: Liquid mass transfer at very low reynolds numbers in packed beds. Ind. Eng. Chem. Fundam. 5, 9–14 (1966)CrossRef Wilson, E.J., Geankoplis, C.J.: Liquid mass transfer at very low reynolds numbers in packed beds. Ind. Eng. Chem. Fundam. 5, 9–14 (1966)CrossRef
go back to reference Wright, P.R., Glasser, B.J.: Modeling mass transfer and hydrodynamics in fluidized-bed adsorption of proteins. Am. Inst. Chem. Eng. J. 47, 474–488 (2001)CrossRef Wright, P.R., Glasser, B.J.: Modeling mass transfer and hydrodynamics in fluidized-bed adsorption of proteins. Am. Inst. Chem. Eng. J. 47, 474–488 (2001)CrossRef
go back to reference Xia, H.F., Lin, D.Q., Yao, S.J.: Chromatographic performance of macroporous cellulose-tungsten carbide composite beads as anion-exchanger for expanded bed adsorption at high fluid velocity. J. Chromatogr. A 1195, 60–66 (2008)CrossRef Xia, H.F., Lin, D.Q., Yao, S.J.: Chromatographic performance of macroporous cellulose-tungsten carbide composite beads as anion-exchanger for expanded bed adsorption at high fluid velocity. J. Chromatogr. A 1195, 60–66 (2008)CrossRef
go back to reference Zhao, J., Yao, S., Lin, D.: Adsorbents for expanded bed adsorption: preparation and functionalization Chinese. J. Chem. Eng. 17, 678–687 (2009) Zhao, J., Yao, S., Lin, D.: Adsorbents for expanded bed adsorption: preparation and functionalization Chinese. J. Chem. Eng. 17, 678–687 (2009)
Metadata
Title
Expanded bed adsorption of albumin and immunoglobulin G from human serum onto a cation exchanger mixed mode adsorbent
Authors
Pedro Ferreira Gomes
José Miguel Loureiro
Alírio E. Rodrigues
Publication date
05-03-2018
Publisher
Springer US
Published in
Adsorption / Issue 3/2018
Print ISSN: 0929-5607
Electronic ISSN: 1572-8757
DOI
https://doi.org/10.1007/s10450-018-9940-7

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