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2019 | OriginalPaper | Chapter

Experimental Evidences Suggest High Between-Vesicle Diversity of Artificial Vesicle Populations: Results, Models and Implications

Authors : Pasquale Stano, Roberto Marangoni, Fabio Mavelli

Published in: Computational Intelligence Methods for Bioinformatics and Biostatistics

Publisher: Springer International Publishing

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Abstract

In the past years, artificial cellular models for origins-of-life research and synthetic biology have been extensively studied. At this aim, solute-filled lipid vesicles (liposomes) are widely used. Several evidences have been collected about the capture of water-soluble chemicals, the mechanism of vesicle self-reproduction, and the course of (bio)chemical reactions in the vesicle lumen. Among the several fascinating questions which emerged from these studies, here we focus on a peculiar feature, namely, the fact that a spontaneous heterogeneity of vesicle structure often emerges. In other words, vesicle populations created in the laboratory by classical batch methods include very ‘diverse’ vesicles with respect to size, morphology, and – importantly – solute content. The consequences of this between-vesicle diversity are shortly discussed.

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previous chapter A Nano Communication System for CTC Detection in Blood Vessels

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Ideally, one would like to have a single lipid monolayer deposited over a large surface so that all parts of the film would experience the same conditions. This corresponds, in most cases, to work well below the \(\mu \)M lipid concentration range, with consequent vesicle losses and other impractical complications. Thus, in the most common experimental conditions the film is rarely so perfect and different regions of the film will experience different micro-environments. Actually, realistic laboratory conditions might affect the measured heterogeneity of vesicle formation paths.

Note that this loss does not refer to the volume loss which follows from the vesicle size reduction, but it is an authentic concentration reduction due to the reduction of the average number per unit of volume.

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Title: Experimental Evidences Suggest High Between-Vesicle Diversity of Artificial Vesicle Populations: Results, Models and Implications
Authors: Pasquale Stano
Roberto Marangoni
Fabio Mavelli
Publisher: Springer International Publishing
Book: Computational Intelligence Methods for Bioinformatics and Biostatistics
Print ISBN: 978-3-030-14159-2

Electronic ISBN: 978-3-030-14160-8

Copyright Year: 2019
DOI: https://doi.org/10.1007/978-3-030-14160-8_17

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