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2014 | OriginalPaper | Chapter

76. Expression, Purification, and Activity Assay of Chicken Interferon-Alpha

Authors : Yue Ma, Minhui Long, Aipo Diao

Published in: Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)

Publisher: Springer Berlin Heidelberg

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Abstract

In this paper, Chicken alpha interferon (IFN-α) gene was cloned into pHis-NusA expression vector, then the recombinant expression vector was transformed into host bacteria E. coli BL21. The recombinant chicken IFN-α was induced to express by IPTG, then the protein expression was analyzed with SDS-PAGE. Under the condition that the recombinant protein was induced to express with 0.1 mM IPTG at 16 °C, the expressed protein was soluble. Recombinant Chicken IFN-α was purified by Ni-metal chelate affinity chromatography. The expressed protein was shown to inhibit the replication of Hand foot and mouth disease virus in vero cells.

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Metadata
Title
Expression, Purification, and Activity Assay of Chicken Interferon-Alpha
Authors
Yue Ma
Minhui Long
Aipo Diao
Copyright Year
2014
Publisher
Springer Berlin Heidelberg
Book
Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)

Print ISBN: 978-3-642-37921-5

Electronic ISBN: 978-3-642-37922-2

Copyright Year: 2014

DOI
https://doi.org/10.1007/978-3-642-37922-2_76

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