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2015 | OriginalPaper | Chapter

56. Extraction and Purification of Lumbrokinase from the “Ohira the 2nd” Earthworm

Authors : Tianjun Li, Jian Ren, Tao Li, Yingchao Wang

Published in: Advances in Applied Biotechnology

Publisher: Springer Berlin Heidelberg

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Abstract

Lumbrokinase is an ideal drug hypertension to cardiovascular diseases. This essay discussed the purification process of lumbrokinase and decided its molecular weight with “Ohira the 2nd” earthworms as raw material. After ammonium sulfate precipitation, the crude enzyme was prepared, which was carried through chromatography of Sephadex G-75 and then DEAE-52. Certain high-purity lumbrokinase components were obtained, whose protein molecular weight was 33KD and specific activity was 1,784 U/mg, after SDS-PAGE test. These lumbrokinase components showed a direct fibrinolytic and plasminogen activation dual role, and a strong thermal stability and pH stability, which was good to thrombolysis.

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Metadata
Title
Extraction and Purification of Lumbrokinase from the “Ohira the 2nd” Earthworm
Authors
Tianjun Li
Jian Ren
Tao Li
Yingchao Wang
Copyright Year
2015
Publisher
Springer Berlin Heidelberg
DOI
https://doi.org/10.1007/978-3-662-45657-6_56

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