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Journal of Sol-Gel Science and Technology

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01-02-2011 | Original paper

FT-IR microscopy characterization of sol–gel layers prior and after glucose oxidase immobilization for biosensing applications

Authors: M. Portaccio, B. Della Ventura, D. G. Mita, N. Manolova, O. Stoilova, I. Rashkov, M. Lepore

Published in: Journal of Sol-Gel Science and Technology | Issue 2/2011

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Abstract

Micro-Attenuated Total Reflection (ATR) Fourier Transform Infrared spectroscopy was used to investigate sol–gel layers for biosensing applications prior and after glucose oxidase (GOD) immobilization. The changes occurring in sol–gel infrared spectrum after GOD immobilization were clearly evidenced confirming the retaining of the enzyme activity. Moreover, micro-ATR experimental technique allowed us to investigate the spatial distribution of enzyme concentration. The non-destructive nature of our approach also enabled to monitor the time stability of sol–gel layers and of embedded GOD. The temporal evolution of some peaks in infrared spectra of these sol–gel layers was compared with absorption and steady-state fluorescence measurements. The results reported here confirm that micro-ATR infrared spectroscopy can be usefully employed for a non- or minimally invasive detailed characterization of supports for enzyme immobilization.

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Pierre AC (2004) Biocatal Biotransformation 22:145CrossRef

Mac Craith BD, McDonagh C, McEvoy AK, Butler T, O’Keeffe G, Murphy V (1997) J Sol-Gel Sci Tech 8:1053CrossRef

Jin W, Brennan JD (2002) Anal Chim Acta 461:1CrossRef

Brinker J, Scherer GW (1990) The physics and chemistry of sol gel processing. Academic Press, New York

Gupta R, Chaudhury N (2007) Biosens Bioelectron 22:2387CrossRef

Tatsu Y, Yamashita K, Yamaguchi M, Yamamura S, Yamamoto H, Yoshikawa S (1992) Chem Lett 21:1615CrossRef

Narang U, Prasad PN, Bright FV, Ramanathan K, Kumar DK, Malhotra BD, Kamalahasan MN, Chandra S (1994) Anal Chem 66:3139CrossRef

Prodromis MI, Karayannis MI (2002) Electroanalysis 14:241CrossRef

Portaccio M, Lepore M, Della Ventura B, Mita DG (2009) J Sol-Gel Sci Technol 50:437CrossRef

10.

Gupta R, Kumar A (2008) Biomed Mater 3:034005CrossRef

11.

Sakka S (2005) Handbook of Sol-gel science and technology: processing, characterization and applications. Kluwer Academic Publisher, New York

12.

Gunzler H, Gremlich HU (2002) IR spectroscopy. Wiley-VCH, Weinheim

13.

Gremlich HU, Yan B (2001) Infrared and Raman spectroscopy of biological materials. Marcel Dekker, New York

14.

Schultz CP (2001) Spectroscopy 16:24

15.

Wetzel DL, Le Vine SM (1999) Science 285:1225CrossRef

16.

Bhargava R, Wall BG, Koenig JL (2000) Appl Spectrosc 54:470CrossRef

17.

Bhargava R, Wang SQ, Koenig JL (2003) Adv Polym Sci 163:137

18.

Sammon C, Boussetta S, Melia C (2002) Macromol Symp 184:357CrossRef

19.

Mei Y, Miller L, Gao W, Gross RA (2003) Biomacromolecules 4:70CrossRef

20.

Jurgen-Lohmann DL, Nacke C, Legge RL, Simon LC (2009) J Sol-Gel Sci Technol 50:77CrossRef

21.

Wilson R, Turner APF (1992) Biosens Bioelectron 7:165CrossRef

22.

Rasband WS, Image J (1997–2006) U. S. National institutes of health, Bethesda, Maryland. http://rsb.info.nih.gov/ij/

23.

Fringuelli UP, Gunthard Hs H (1982) Infrared membrane spectroscopy. In: Grell E (ed) Membrane spectroscopy. Springer, Berlin, pp 270–332

24.

Applications and Design of a Micro-ATR objective technical note Perkin Elmer

25.

Nocun M, Leja E, Jedlinski L, Najman J (2005) J Mol Struct 744–747:597CrossRef

26.

Parill TM (1992) J Mater Res 7:2230CrossRef

27.

Mauritz KA, Warren RM (1989) Macromolecules 22:1731

28.

Smith B (1999) Infrared spectral interpretation. A systematic approach. CRC Press, Washington

29.

Lin-Vien D, Colthup NB, Fateley WG, Grasselli JC (1991) The handbook of infrared and Raman characteristic frequencies of organic molecules. Academic Press, San Diego

30.

Coates J (2000) Interpretation of infrared spectra a practical. Approach J. Wiley and Sons Ltd, Chichester

31.

Barth A (2007) Biochim Biophys Acta 1767:1073CrossRef

32.

Krimm S, Bandekar J (1986) Adv Protein Chem 38:181CrossRef

33.

Susi H, Byler DM (1986) Methods Enzymol 130:290CrossRef

34.

Bandekar J (1992) Biochim Biophys Acta Protein Struct Mol Enzymol 1120(2):123CrossRef

35.

Zuo S, Teng Y, Yuan H, Lan M (2008) Sens Actuators B 133:555CrossRef

36.

Blanco M, Cruz J, Bautista M (2008) Anal Bioanal Chem 392:1367CrossRef

37.

Asuero AG, Sayago A, Gonzalez AG (2006) Crit Rev Anal Chem 36:41CrossRef

38.

Schaferling S, Wu M, Wolfbeis OS (2004) J Fluoresc 14:561CrossRef

39.

Applications and design of a Micro-ATR objective for FT-IR imaging. Technical note Perkin Elmer

Title: FT-IR microscopy characterization of sol–gel layers prior and after glucose oxidase immobilization for biosensing applications
Authors: M. Portaccio
B. Della Ventura
D. G. Mita
N. Manolova
O. Stoilova
I. Rashkov
M. Lepore
Publication date: 01-02-2011
Publisher: Springer US
Published in: Journal of Sol-Gel Science and Technology / Issue 2/2011
Print ISSN: 0928-0707
Electronic ISSN: 1573-4846
DOI: https://doi.org/10.1007/s10971-010-2343-1

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