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Journal of Nanoparticle Research

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01-08-2012 | Research Paper

Gene expression profiling in rat kidney after intratracheal exposure to cadmium-doped nanoparticles

Authors: Teresa Coccini, Elisa Roda, Marco Fabbri, Maria Grazia Sacco, Laura Gribaldo, Luigi Manzo

Published in: Journal of Nanoparticle Research | Issue 8/2012

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Abstract

While nephrotoxicity of cadmium is well documented, very limited information exists on renal effects of exposure to cadmium-containing nanomaterials. In this work, “omics” methodologies have been used to assess the action of cadmium-containing silica nanoparticles (Cd-SiNPs) in the kidney of Sprague-Dawley rats exposed intratracheally. Groups of animals received a single dose of Cd-SiNPs (1 mg/rat), CdCl₂ (400 μg/rat) or 0.1 ml saline (control). Renal gene expression was evaluated 7 and 30 days post exposure by DNA microarray technology using the Agilent Whole Rat Genome Microarray 4x44K. Gene modulating effects were observed in kidney at both time periods after treatment with Cd-SiNPs. The number of differentially expressed genes being 139 and 153 at the post exposure days 7 and 30, respectively. Renal gene expression changes were also observed in the kidney of CdCl₂-treated rats with a total of 253 and 70 probes modulated at 7 and 30 days, respectively. Analysis of renal gene expression profiles at day 7 indicated in both Cd-SiNP and CdCl₂ groups downregulation of several cluster genes linked to immune function, oxidative stress, and inflammation processes. Differing from day 7, the majority of cluster gene categories modified by nanoparticles in kidney 30 days after dosing were genes implicated in cell regulation and apoptosis. Modest renal gene expression changes were observed at day 30 in rats treated with CdCl₂. These results indicate that kidney may be a susceptible target for subtle long-lasting molecular alterations produced by cadmium nanoparticles locally instilled in the lung.

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Title: Gene expression profiling in rat kidney after intratracheal exposure to cadmium-doped nanoparticles
Authors: Teresa Coccini
Elisa Roda
Marco Fabbri
Maria Grazia Sacco
Laura Gribaldo
Luigi Manzo
Publication date: 01-08-2012
Publisher: Springer Netherlands
Published in: Journal of Nanoparticle Research / Issue 8/2012
Print ISSN: 1388-0764
Electronic ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-012-0925-2

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