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2015 | Online First | Chapter

Generation and Characterization of Rat iPSCs

Authors : Jing Liao, Chun Cui

Published in: Methods in Molecular Biology™

Publisher: Springer New York

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Abstract

The laboratory rat (Rattus norvegicus) is now on the leading edge used as a laboratory model system to study pharmacology, toxicology, immunology, nutrition, behavior, and numerous other topics. Therefore, generation of rat induced pluripotent stem cells (iPSCs) through somatic cells reprogramming is a powerful tool for establishing in vitro disease model, development of new protocols for treatment of different diseases, and creating transgenic rat models. Here, we describe a simple adopted protocol for establishing rat iPSCs from different types of somatic cells including rat primary ear fibroblast (PEF) and primary bone marrow cells (BMC).

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Title: Generation and Characterization of Rat iPSCs
Authors: Jing Liao
Chun Cui
Publisher: Springer New York
DOI: https://doi.org/10.1007/7651_2015_200

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Abstract

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