Top

Published in:

2015 | Online First | Chapter

Generation of Human Induced Pluripotent Stem Cells Using RNA-Based Sendai Virus System and Pluripotency Validation of the Resulting Cell Population

Authors : Valeria Chichagova, Irene Sanchez-Vera, Lyle Armstrong, David Steel, Majlinda Lako

Published in: Methods in Molecular Biology™

Publisher: Springer New York

Activate our intelligent search to find suitable subject content or patents.

search-config

AI-assisted search

Off

Abstract

Human induced pluripotent stem cells (hiPSCs) provide a platform for studying human disease in vitro, increase our understanding of human embryonic development, and provide clinically relevant cell types for transplantation, drug testing, and toxicology studies. Since their discovery, numerous advances have been made in order to eliminate issues such as vector integration into the host genome, low reprogramming efficiency, incomplete reprogramming and acquisition of genomic instabilities. One of the ways to achieve integration-free reprogramming is by using RNA-based Sendai virus. Here we describe a method to generate hiPSCs with Sendai virus in both feeder-free and feeder-dependent culture systems. Additionally, we illustrate methods by which to validate pluripotency of the resulting stem cell population.

Evans MJ, Kaufman MH (1981) Establishment in culture of pluripotential cells from mouse embryos. Nature 292(5819):154–156CrossRefPubMed

Martin GR (1981) Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. Proc Natl Acad Sci U S A 78(12):7634–7638PubMedCentralCrossRefPubMed

Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, Jones JM (1998) Embryonic stem cell lines derived from human blastocysts. Science 282(5391):1145–1147CrossRefPubMed

Thomson JA, Marshall VS (1998) Primate embryonic stem cells. Curr Top Dev Biol 38:133–165CrossRefPubMed

Preynat-Seauve O, de Rham C, Tirefort D, Ferrari-Lacraz S, Krause KH, Villard J (2009) Neural progenitors derived from human embryonic stem cells are targeted by allogeneic T and natural killer cells. J Cell Mol Med 13(9B):3556–3569. doi:10.1111/j.1582-4934.2009.00746.x PubMedCentralCrossRefPubMed

Yu J, Vodyanik MA, Smuga-Otto K, Antosiewicz-Bourget J, Frane JL, Tian S, Nie J, Jonsdottir GA, Ruotti V, Stewart R, Slukvin II, Thomson JA (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318(5858):1917–1920. doi:10.1126/science.1151526 CrossRefPubMed

Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131(5):861–872. doi:10.1016/j.cell.2007.11.019 CrossRefPubMed

Yamanaka S (2009) Elite and stochastic models for induced pluripotent stem cell generation. Nature 460(7251):49–52. doi:10.1038/nature08180 CrossRefPubMed

Smith ZD, Nachman I, Regev A, Meissner A (2010) Dynamic single-cell imaging of direct reprogramming reveals an early specifying event. Nat Biotechnol 28(5):521–526. doi:10.1038/nbt.1632 PubMedCentralCrossRefPubMed

10.

Buganim Y, Faddah DA, Cheng AW, Itskovich E, Markoulaki S, Ganz K, Klemm SL, van Oudenaarden A, Jaenisch R (2012) Single-cell expression analyses during cellular reprogramming reveal an early stochastic and a late hierarchic phase. Cell 150(6):1209–1222. doi:10.1016/j.cell.2012.08.023 PubMedCentralCrossRefPubMed

11.

Polo JM, Anderssen E, Walsh RM, Schwarz BA, Nefzger CM, Lim SM, Borkent M, Apostolou E, Alaei S, Cloutier J, Bar-Nur O, Cheloufi S, Stadtfeld M, Figueroa ME, Robinton D, Natesan S, Melnick A, Zhu J, Ramaswamy S, Hochedlinger K (2012) A molecular roadmap of reprogramming somatic cells into iPS cells. Cell 151(7):1617–1632. doi:10.1016/j.cell.2012.11.039 PubMedCentralCrossRefPubMed

12.

Briggs R, King TJ (1952) Transplantation of living nuclei from blastula cells into enucleated frogs’ eggs. Proc Natl Acad Sci U S A 38(5):455–463PubMedCentralCrossRefPubMed

13.

Gurdon JB (1962) The developmental capacity of nuclei taken from intestinal epithelium cells of feeding tadpoles. J Embryol Exp Morphol 10:622–640PubMed

14.

Robl JM, Gilligan B, Critser ES, First NL (1986) Nuclear transplantation in mouse embryos: assessment of recipient cell stage. Biol Reprod 34(4):733–739CrossRefPubMed

15.

Tsunoda Y, Yasui T, Shioda Y, Nakamura K, Uchida T, Sugie T (1987) Full-term development of mouse blastomere nuclei transplanted into enucleated two-cell embryos. J Exp Zool 242(2):147–151. doi:10.1002/jez.1402420205 CrossRefPubMed

16.

Campbell KH, McWhir J, Ritchie WA, Wilmut I (1996) Sheep cloned by nuclear transfer from a cultured cell line. Nature 380(6569):64–66. doi:10.1038/380064a0 CrossRefPubMed

17.

Wilmut I, Schnieke AE, McWhir J, Kind AJ, Campbell KH (1997) Viable offspring derived from fetal and adult mammalian cells. Nature 385(6619):810–813. doi:10.1038/385810a0 CrossRefPubMed

18.

Rideout WM 3rd, Hochedlinger K, Kyba M, Daley GQ, Jaenisch R (2002) Correction of a genetic defect by nuclear transplantation and combined cell and gene therapy. Cell 109(1):17–27CrossRefPubMed

19.

Hochedlinger K, Jaenisch R (2002) Monoclonal mice generated by nuclear transfer from mature B and T donor cells. Nature 415(6875):1035–1038. doi:10.1038/nature718 CrossRefPubMed

20.

Byrne JA, Pedersen DA, Clepper LL, Nelson M, Sanger WG, Gokhale S, Wolf DP, Mitalipov SM (2007) Producing primate embryonic stem cells by somatic cell nuclear transfer. Nature 450(7169):497–502. doi:10.1038/nature06357 CrossRefPubMed

21.

Tachibana M, Amato P, Sparman M, Gutierrez NM, Tippner-Hedges R, Ma H, Kang E, Fulati A, Lee HS, Sritanaudomchai H, Masterson K, Larson J, Eaton D, Sadler-Fredd K, Battaglia D, Lee D, Wu D, Jensen J, Patton P, Gokhale S, Stouffer RL, Wolf D, Mitalipov S (2013) Human embryonic stem cells derived by somatic cell nuclear transfer. Cell 153(6):1228–1238. doi:10.1016/j.cell.2013.05.006 PubMedCentralCrossRefPubMed

22.

Park HJ, Shin J, Kim J, Cho SW (2013) Nonviral delivery for reprogramming to pluripotency and differentiation. Arch Pharm Res 37(1):107–119. doi:10.1007/s12272-013-0287-z CrossRefPubMed

23.

Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA (2009) Human induced pluripotent stem cells free of vector and transgene sequences. Science 324(5928):797–801. doi:10.1126/science.1172482 PubMedCentralCrossRefPubMed

24.

Chou BK, Mali P, Huang X, Ye Z, Dowey SN, Resar LM, Zou C, Zhang YA, Tong J, Cheng L (2011) Efficient human iPS cell derivation by a non-integrating plasmid from blood cells with unique epigenetic and gene expression signatures. Cell Res 21(3):518–529. doi:10.1038/cr.2011.12 PubMedCentralCrossRefPubMed

25.

Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong H, Nakagawa M, Tanabe K, Tezuka K, Shibata T, Kunisada T, Takahashi M, Takahashi J, Saji H, Yamanaka S (2011) A more efficient method to generate integration-free human iPS cells. Nat Methods 8(5):409–412. doi:10.1038/nmeth.1591 CrossRefPubMed

26.

Jia F, Wilson KD, Sun N, Gupta DM, Huang M, Li Z, Panetta NJ, Chen ZY, Robbins RC, Kay MA, Longaker MT, Wu JC (2010) A nonviral minicircle vector for deriving human iPS cells. Nat Methods 7(3):197–199. doi:10.1038/nmeth.1426 PubMedCentralCrossRefPubMed

27.

Narsinh KH, Jia F, Robbins RC, Kay MA, Longaker MT, Wu JC (2011) Generation of adult human induced pluripotent stem cells using nonviral minicircle DNA vectors. Nat Protoc 6(1):78–88. doi:10.1038/nprot.2010.173 PubMedCentralCrossRefPubMed

28.

Cho HJ, Lee CS, Kwon YW, Paek JS, Lee SH, Hur J, Lee EJ, Roh TY, Chu IS, Leem SH, Kim Y, Kang HJ, Park YB, Kim HS (2010) Induction of pluripotent stem cells from adult somatic cells by protein-based reprogramming without genetic manipulation. Blood 116(3):386–395. doi:10.1182/blood-2010-02-269589 CrossRefPubMed

29.

Zhou H, Wu S, Joo JY, Zhu S, Han DW, Lin T, Trauger S, Bien G, Yao S, Zhu Y, Siuzdak G, Scholer HR, Duan L, Ding S (2009) Generation of induced pluripotent stem cells using recombinant proteins. Cell Stem Cell 4(5):381–384. doi:10.1016/j.stem.2009.04.005 CrossRefPubMed

30.

Kim D, Kim CH, Moon JI, Chung YG, Chang MY, Han BS, Ko S, Yang E, Cha KY, Lanza R, Kim KS (2009) Generation of human induced pluripotent stem cells by direct delivery of reprogramming proteins. Cell Stem Cell 4(6):472–476. doi:10.1016/j.stem.2009.05.005 PubMedCentralCrossRefPubMed

31.

Yakubov E, Rechavi G, Rozenblatt S, Givol D (2010) Reprogramming of human fibroblasts to pluripotent stem cells using mRNA of four transcription factors. Biochem Biophys Res Commun 394(1):189–193. doi:10.1016/j.bbrc.2010.02.150 CrossRefPubMed

32.

Warren L, Manos PD, Ahfeldt T, Loh YH, Li H, Lau F, Ebina W, Mandal PK, Smith ZD, Meissner A, Daley GQ, Brack AS, Collins JJ, Cowan C, Schlaeger TM, Rossi DJ (2010) Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA. Cell Stem Cell 7(5):618–630. doi:10.1016/j.stem.2010.08.012 PubMedCentralCrossRefPubMed

33.

Zhou W, Freed CR (2009) Adenoviral gene delivery can reprogram human fibroblasts to induced pluripotent stem cells. Stem Cells 27(11):2667–2674. doi:10.1002/stem.201 CrossRefPubMed

34.

Kuroya M, Ishida N (1953) Newborn virus pneumonitis (type Sendai). II. The isolation of a new virus possessing hemagglutinin activity. Yokohama Med Bull 4(4):217–233PubMed

35.

Li HO, Zhu YF, Asakawa M, Kuma H, Hirata T, Ueda Y, Lee YS, Fukumura M, Iida A, Kato A, Nagai Y, Hasegawa M (2000) A cytoplasmic RNA vector derived from nontransmissible Sendai virus with efficient gene transfer and expression. J Virol 74(14):6564–6569PubMedCentralCrossRefPubMed

36.

Fusaki N, Ban H, Nishiyama A, Saeki K, Hasegawa M (2009) Efficient induction of transgene-free human pluripotent stem cells using a vector based on Sendai virus, an RNA virus that does not integrate into the host genome. Proc Jpn Acad Ser B Phys Biol Sci 85(8):348–362PubMedCentralCrossRefPubMed

37.

Seki T, Yuasa S, Oda M, Egashira T, Yae K, Kusumoto D, Nakata H, Tohyama S, Hashimoto H, Kodaira M, Okada Y, Seimiya H, Fusaki N, Hasegawa M, Fukuda K (2010) Generation of induced pluripotent stem cells from human terminally differentiated circulating T cells. Cell Stem Cell 7(1):11–14. doi:10.1016/j.stem.2010.06.003 CrossRefPubMed

38.

Nishishita N, Shikamura M, Takenaka C, Takada N, Fusaki N, Kawamata S (2012) Generation of virus-free induced pluripotent stem cell clones on a synthetic matrix via a single cell subcloning in the naive state. PLoS One 7(6):e38389. doi:10.1371/journal.pone.0038389 PubMedCentralCrossRefPubMed

39.

Ban H, Nishishita N, Fusaki N, Tabata T, Saeki K, Shikamura M, Takada N, Inoue M, Hasegawa M, Kawamata S, Nishikawa S (2011) Efficient generation of transgene-free human induced pluripotent stem cells (iPSCs) by temperature-sensitive Sendai virus vectors. Proc Natl Acad Sci U S A 108(34):14234–14239. doi:10.1073/pnas.1103509108 PubMedCentralCrossRefPubMed

40.

Garfield AS (2010) Derivation of primary mouse embryonic fibroblast (PMEF) cultures. Methods Mol Biol 633:19–27. doi:10.1007/978-1-59745-019-5_2 CrossRefPubMed

Title: Generation of Human Induced Pluripotent Stem Cells Using RNA-Based Sendai Virus System and Pluripotency Validation of the Resulting Cell Population
Authors: Valeria Chichagova
Irene Sanchez-Vera
Lyle Armstrong
David Steel
Majlinda Lako
Publisher: Springer New York
DOI: https://doi.org/10.1007/7651_2015_205

Springer Professional

Abstract

Please log in to get access to your license.