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2014 | OriginalPaper | Chapter

4. High Performance Thin-Layer Chromatography

Authors : Dušanka Milojković-Opsenica, Filip Andrić

Published in: Green Chromatographic Techniques

Publisher: Springer Netherlands

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Abstract

The current chapter emphasizes the use of high-performance thin-layer chromatography (HPTLC) as a green analytical separation alternative. The chapter provides historical development of thin-layer chromatography towards becoming modern, automated, high resolution technique in the form of high-performance thin-layer chromatography, and their further advances in miniaturization of chromatographic beds in the form of ultra-performance thin-layer chromatography (UPTLC). Also, following the three R philosophy (reduce, replace, reuse), and twelve principles of green chemistry, the chapter provides the short review of green sample pretreatment techniques coupled with the HPTLC. In addition, the use of green separation modalities such as reversed-phase chromatography, hydrophilic interaction chromatography and salting out thin-layer chromatography is emphasized. The use of thin-layer chromatography as a simple screening technique and a powerful sample pretreatment method is highlighted.

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previous chapter Supercritical Fluid Chromatography: A Green Approach for Separation and Purification of Organic and Inorganic Analytes

next chapter Green Techniques in Gas Chromatography

Aleksić MB, Agbaba DG, Milojković-Opsenica DM et al (2001) Salting-out thin-layer chromatography of some angiotensin converting enzyme inhibitors on different sorbents. Chromatographia 53(S):442–444

Aleksić M, Odović J, Milojković-Opsenica DM et al (2003) Salting-out thin-layer chromatography of several myorelaxants. J Planar Chromatogr—Mod TLC 16(2):144–146

Ali I, Hussain I, Saleem K et al (2012) Development of efficient SPE–TLC method and evaluation of biological interactions of contraceptives with progesterone receptors. Arabian J Chem 5:235–240CrossRef

Alpert AJ (1990) Hydrophilic-interaction chromatography for the separation of peptides, nucleic acids and other polar compounds. J Chromatogr A 499:177–196CrossRef

Anastas PT (1999) Green chemistry and the role of analytical methodology development. Crit Rev Anal Chem 29(3):167–175CrossRef

Anastas PT, Warner JC (1998) Green chemistry: theory and practice. Oxford University Press, New York

Andrić FLj, Trifkovic JĐ, Radoičić AD et al (2010) Determination of the soil–water partition coefficients (log KOC) of some mono- and poly-substituted phenols by reversed-phase thin-layer chromatography. Chemosphere 81(3):299–305CrossRef

Bate-Smith EC, Westall RG (1950) Chromatographic behavior and chemical structure. 1. Some naturally occurring phenolic substances. Biochim Biophys Acta 4:427–440CrossRef

Bazylak G, Brózik H, Sabanty W (2000) Combined SPE and HPTLC as a screening assay of urinary cotinine from male adolescents exposed to environmental tobacco smoke. Pol J Environ Stud 9(2):113–123

Bezuidenhout LW, Brett MJ (2008) Ultrathin layer chromatography on nanostructured thin films. J Chromatogr A 1183:179–185

Bhandari P, Kumar N, Gupta AP et al (2007) A rapid RP-HPTLC densitometry method for simultaneous determination of major flavonoids in important medicinal plants. J Sep Sci 30:2092–2096CrossRef

Bhope SG, Ghosh VK, Kuber VV (2011) Rapid microwave-assisted extraction and HPTLC photodensitometric method for the quality assessment of Boerhaavia diffusa L. J AOAC Int 94(3):795–802

Búriová E, Macásek F, Melichar F, Kropácek M, Procházka L (2005) Autoradiolysis of the 2-deoxy-2-[18F] fluoro-D-glucose radiopharmaceutical. J Radioanal Nucl Chem 264 (3):595–602CrossRef

Capello C, Fischer U, Hungerbühler K (2007) What is a green solvent? A comprehensive framework for the environmental assessment of solvents. Green Chem 9:927–934CrossRef

Cieśla Ł, Hajnos M, Waksmundzka-Hajnos M (2011) Application of hydrophilic interaction TLC systems for separation of highly polar glycosidic compounds from the flowers of selected verbascum species. J Planar Chromatogr—Mod TLC 24(4):295–300

Clark JE, Olesik SV (2009) Technique for ultrathin layer chromatography using an electrospun, nanofibrous stationary phase. Anal Chem 81:4121–4129CrossRef

Dabić D, Natić M, Džambaski Z et al (2011) Quantitative structure-retention relationship of new N-substituted 2-alkylidene-4-oxothiazolidines. J Sep Sci 34(18):2397–2404CrossRef

Faraji H, Saber-Tehrani M, Mirzaie A et al (2011) Application of liquid–liquid microextraction–high-performance thin-layer chromatography for preconcentration and determination of phenolic compounds in aqueous samples. J Planar Chromatogr 24(3):214–217CrossRef

Flieger J, Swieboda R, Tatarczak M (2007) Chemometric analysis of retention data from salting-out thin-layer chromatography in relation to structural parameters and biological activity of chosen sulphonamides. J Chromatogr B 846(1–2):334–340CrossRef

Frolova AM, Konovalova OY, Loginova LP et al (2011) Thin-layer chromatographic plates with monolithic layer of silica: production, physical–chemical characteristics, separation capabilities. J Sep Sci 34:2352–2361

Gandhi DB, Mehta PJ (2011) Simultaneous RP-HPTLC method for determination of Levodopa, Carbidopa, and Entacapone in combined tablet dosage form. J Planar Chromatogr -Mod TLC 24(3):236–241CrossRef

Giaginis C, Tsantili-Kakoulidou A (2008) Alternative measures of lipophilicity: from octanol-water partitioning to IAM retention. J Pharm Sci 97(8):2984–3004CrossRef

Greibrokk T, Andersen T (2003) High-temperature liquid chromatography. J Chromatogr A 1000:743–755

Grover PK, Ryall RL (2005) Critical appraisal of salting-out and its implications for chemical and biological sciences. Chem Rev 105(1):2–10CrossRef

De la Guardia M, Ruzicka J (1995) Towards environmentally conscientious analytical chemistry through miniaturization, containment and reagent replacement. Analyst 120:17N

De la Guardia M, Khalaf KD, Carbonell V et al (1995) Clean analytical method for the determination of propoxur. Anal Chim Acta 308:462–468CrossRef

Guillarme D, Heinisch S, Rocca JL (2004) Effect of temperature in reversed phase liquid chromatography. J Chromatogr A 1052:39–51

Gupta S, Shanker K, Srivastava SK (2012) HPTLC method for the simultaneous determination of four indole alkaloids in Rauwolfia tetraphylla: a study of organic/green solvent and continuous/pulse sonication. J Pharm Biomed Anal 66:33–39CrossRef

Hartonen K, Riekkola M (2008) Liquid chromatography at elevated temperatures with pure water as the mobile phase. Trends Anal Chem 27:1–14CrossRef

Jandera P (2008) Stationary phases for hydrophilic interaction chromatography, their characterization and implementation into multidimensional chromatography concepts. J Sep Sci 31:1421–1437CrossRef

Jevric LR, Koprivtca GB, Misljenovic NM et al (2010) Chromatographic behavior and lipophilicity of s-triazine derivatives on silica gel impregnated with paraffin oil. Acta Period Technol 41:159–168CrossRef

Jim SR, Oko AJ, Taschuk MT et al (2011) Morphological modification of nanostructured ultrathin-layer chromatography stationary phases. J Chromatogr A 1218:7203–7210

Kirchner JG (1967) Thin layer chromatography. In: Perry ES, Weissberger A (eds) Techniques of organic chemistry, 1st edn. Wiley, New York

Kochana J, Parczewski A, Wilamowski J (2006) SPE/TLC profiling of the impurities of MDMA: the influence of an agglutinant, diluents, and adulterants. J Liq Chromatogr Related Technol 29:1247–1256CrossRef

Kowalska T, Kaczmarski K, Prus W (2003) Theory and mechanism of thin-layer chromatography. In: Sherma J, Fried B (eds) Handbook of thin-layer chromatography, 3rd edn. Marcel Dekker, New York-Basel

Krzek J, Starek M, Jelonkiewicz D (2005) RP-TLC determination of S(+) and R(–) ibuprofen in drugs with the application of chiral mobile phase and UV densitometric detection. Chromatographia 62:653–657CrossRef

Kutsch H, Schoen U (2000) A simplified HPTLC screening method for the estimation of the PAH content in soil samples. Fresenius J Anal Chem 367:279–283CrossRef

Lin M-C, Tsai M-J, Wen K-C (1999) Supercritical fluid extraction of flavonoids from Scutellariae Radix. J Chromatogr A 830:387–395CrossRef

Malik I, Sedlarova E, Andriamainty F et al (2011) Synthesis and basic physicochemical properties of 1-[3-(Y-alkoxyphenylcarbamoyloxy)-2-hydroxypropyl]-4-(2-methylphenyl)piperazine chlorides. Ceska a Slovenska Farmacie 60(3):137–143

Mecozzi M, Amici M, Romanelli G et al (2002) Ultrasound extraction and thin layer chromatography–flame ionization detection analysis of the lipid fraction in marine mucilage samples. J Chromatogr A 963:363–373CrossRef

Miller JM (2005) Chromatography: concepts and contrasts. Wiley, New York

Milojković-Opsenica DM, Malinar MJ, Tešić ŽLj (1999) Effect of the chelate ring size of diamine–Co(III) complexes on their hR_F values obtained under salting-out thin-layer chromatography conditions. J Chromatogr A 847:291–296CrossRef

Miszczyk M, Pyka A (2006) Comparison of normal and reversed-phase TLC for separation of selected pesticides. J Planar Chromatogr 19:15–20CrossRef

Mohammad A, Laeeq S (2011) Identification of coexisting pentose, hexose, and disaccharides with preliminary separation through hydrophilic interaction on silica HPTLC plate using aqueous sodium deoxycholate–acetonitrile mobile phase system. J Planar Chromatogr—Mod TLC 24 (6):491–496

Morlock G, Schwack W (2008) Planar chromatography—back to the future? LC-GC Eur 21:366–371

Nascu-Briciu RD, Sarbu C (2010) The lipophilicity determination of some pesticides by high performance thin-layer chromatography and various computing methods. Studia Universitatis Babes-Bolyai, Chemia 55(3):105–117

Odović JV, Stojimirović BB, Aleksić MB et al (2005) Examination of the Hydrophobicity of ACE inhibitors and their active metabolites by salting-out thin-layer chromatography. J Planar Chromatogr—Mod TLC 18(102):98–103

Oellig C, Schwack W (2011) Planar solid phase extraction—a new clean-up concept in multi-residue analysis of pesticides by liquid chromatography–mass spectrometry. J Chromatogr A 1218:6540–6547

Phattanawasin P, Sotanaphun U, Sukwattanasinit T et al (2012) Quantitative determination of sibutramine in adulterated herbal slimming formulations by TLC-image analysis method. Forensic Sci Int 219:96–100CrossRef

Pobłocka-Olech L, Krauze-Baranowska M (2008) SPE-HPTLC of procyanidins from the barks of different species and clones of Salix. J Pharm Biomed Anal 48:965–968CrossRef

Poole CF (2003) Thin-layer chromatography: challenges and opportunities. J Chromatogr A 1000:963–984

Poole SK, Poole CF (2011) High performance stationary phases for planar chromatography. J Chromatogr A 1218:2648–2660

Radoičić A, Majstorović H, Sabo T et al (2009) Hydrophilic-interaction planar chromatography of some water-soluble Co(III)-complexes on different adsorbents. J Planar Chromatogr—Mod TLC 22 (4):249–253

Rezić I, Horvat AJM, Babić S et al (2005) Determination of pesticides in honey by ultrasonic solvent extraction and thin-layer chromatography. Ultrason Sonochem 12:477–481CrossRef

Ribeiro RLV, Bottoli CB, Collins KE et al (2004) Re-evaluation of ethanol as organic modifier for use in HPLC-RP mobile phases. J Brazil Chem Soc 15:300–306CrossRef

Sarbu C, Djakovic-Sekulic T, Perisic-Janjic N (2002) Evaluation of lipophilicity of some benzimidazole and benzotriazole derivatives by RP HPTLC and PCA. J Pharm Biomed Anal 30:739–745CrossRef

Šegan S, Andrić F, Radoičić A et al (2011) Correlation between structure, retention and activity of cholic acid derived cis-trans isomeric bis-steroidal tetraoxanes. J Sep Sci 34(19):2659–2667CrossRef

Sharma UK, Sharma N, Gupta AP et al (2007) RP-HPTLC densitometric determination and validation of vanillin and related phenolic compounds in accelerated solvent extract of Vanilla planifolia. J Sep Sci 30:3174–3180CrossRef

Sherma J (2003) Basic TLC techniques, materials, and apparatus. In: Sherma J, Fried B (eds) Handbook of thin-layer chromatography, 3rd edn. Marcel Dekker, New York-Basel

Shewiyo DH, Kaale E, Risha PG et al (2012) HPTLC methods to assay active ingredients in pharmaceutical formulations: a review of the method development and validation steps. J Pharm Biomed Anal 66:11–23CrossRef

Shweshein AMSK, Radoičić A, Andrić F et al (2012) Hydrophilic interaction planar chromatography of geometrical isomers of some Co(III) complexes. J Liq Chromatogr Related Technol 35:1289–1297

Siouffi A-M (2005) From paper to planar: 60 years of thin layer chromatography. Sep Purif Rev 34:155–180CrossRef

Skibinski R, Slawik T, Kaczkowska M (2011) Reversed-phase TLC study of the lipophilicity of fourteen 1,3-benzoxazol-2(3H)-one derivatives and comparison with isomeric 1,2-benzisoxazol-3(2H)-one analogs. J Planar Chromatogr—Mod TLC 24(4):348–351

Smith RM (2006) Superheated water: the ultimate green solvent for separation science. Anal Bioanal Chem 385:419–421CrossRef

Stahl E (1956) Thin-Layer Chromatography. Pharmazie 11:633–637

Trifkovic J, Andric F, Ristivojevic P et al (2010) Structure-retention relationship study of arylpiperazines by linear multivariate modeling. J Sep Sci 33(17-18):2619–2628CrossRef

Tsantili-Kakoulidou A (2005) Lipophilicity: assessment by reversed-phase TLC and HPLC. In: Encyclopedia of chromatography, 2nd edn, CRC Press. 993–998

Tuzimski T (2010) Application of HPLC and TLC with diode array detection after SPE to the determination of pesticides in water samples from the Zemborzycki reservoir (Lublin, Southeastern Poland). J AOAC Int 93(6):1748–1756

Vailaya A (2005) Fundamentals of reversed phase chromatography: thermodynamic and exothermodynamic treatment. J Liq Chromatogr Related Technol 28:965–1054CrossRef

Vučković G, Živković-Radovanović V (2008) Use of different salt solutions in salting-out TLC of Co(III) complexes on silica gel. Chromatographia 67:259–267CrossRef

Wang QS, Zhang L (1999) Review of research on quantitative structure-retention relationships in thin-layer chromatography. J Liq Chromatogr Related Technol 22(1):1–14CrossRef

Welch CJ, Brkovic T, Schafer W et al (2009) Performance to burn? Re-evaluating the choice of acetonitrile as the platform solvent for analytical HPLC. Green Chem 11:1232–1238CrossRef

Wu J, Lin L, Chao F (2001) Ultrasound assisted extraction of ginseng saponins from ginseng roots and ginseng cells. Ultrason Sonochem 8:347–352CrossRef

Title: High Performance Thin-Layer Chromatography
Authors: Dušanka Milojković-Opsenica
Filip Andrić
Publisher: Springer Netherlands
Book: Green Chromatographic Techniques
Print ISBN: 978-94-007-7734-7

Electronic ISBN: 978-94-007-7735-4

Copyright Year: 2014
DOI: https://doi.org/10.1007/978-94-007-7735-4_4

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