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Hough Based Evolutions for Enhancing Structures in 3D Electron Microscopy

  • 2019
  • OriginalPaper
  • Chapter
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Abstract

Connecting interrupted line-like structures is a frequent problem in image processing. Here we focus on the specific needs that occur in 3D biophysical data analysis in electron microscopy (EM). We introduce a powerful framework for connecting line-like structures in 3D data sets by combining a specific semilocal Hough transform with a directional evolution equation. The Hough transform allows to find the principal orientations of the local structures in a robust way, and the evolution equation is designed as a partial differential equation that smoothes along these principal orientations. We evaluate the performance of our method for enhancing structures in both synthetic and real-world EM data. In contrast to traditional structure tensor based methods such as coherence-enhancing diffusion, our method can handle the missing wedge problem in EM, also known as limited angle tomography problem. A modified version of our approach is also able to tackle the discontinuities created due to the contrast transfer function correction of EM images.

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Title
Hough Based Evolutions for Enhancing Structures in 3D Electron Microscopy
Authors
Kireeti Bodduna
Joachim Weickert
Achilleas S. Frangakis
Copyright Year
2019
DOI
https://doi.org/10.1007/978-3-030-29888-3_9
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