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2013 | OriginalPaper | Chapter

12. Human Sperm Tracking, Analysis, and Manipulation

Authors : Jun Liu, Clement Leung, Zhe Lu, Yu Sun

Published in: Smart Materials-Based Actuators at the Micro/Nano-Scale

Publisher: Springer New York

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Abstract

Sperm analysis and manipulation play a significant role in biology research and reproductive medicine (assisted reproductive technologies). This chapter reviews computer vision-based sperm tracking methods, sperm analysis techniques, and automated sperm manipulation. Based on computer vision tracking of sperm head and sperm tail, sperm motility can be quantified by calculating the sperm’s straight line velocity, curvilinear velocity, moving path linearity, and the sperm tail beating amplitude. Conventional computer-assisted sperm analysis (CASA) systems are capable of performing some of these tasks. Recent progress in this field provides additional, enhanced capabilities to biologists and clinical embryologists. This chapter also introduces recent progress in automating sperm manipulation procedures, including sperm immobilization, aspiration, and positioning inside a micropipette.

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Literature
1.
go back to reference D.F. Katz, J.W. Overstreet, S.J. Samuels, P.W. Niswander, T.D. Bloom, E.L. Lewis, Morphometric analysis of spermatozoa in the assessment of human male fertility. J. Androl. 7, 203–210 (1986) D.F. Katz, J.W. Overstreet, S.J. Samuels, P.W. Niswander, T.D. Bloom, E.L. Lewis, Morphometric analysis of spermatozoa in the assessment of human male fertility. J. Androl. 7, 203–210 (1986)
2.
go back to reference G. Palermo, H. Joris, P. Devroey, A.C. Van Steirteghem, Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet 340, 17–18 (1992)CrossRef G. Palermo, H. Joris, P. Devroey, A.C. Van Steirteghem, Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet 340, 17–18 (1992)CrossRef
3.
go back to reference T.G. Cooper et al., World Health Organization reference values for human semen characteristics. Hum. Reprod. Update 16, 231–245 (2009)CrossRef T.G. Cooper et al., World Health Organization reference values for human semen characteristics. Hum. Reprod. Update 16, 231–245 (2009)CrossRef
4.
go back to reference B. Bartoov, A. Berkovitz, F. Eltes, A. Kogosovsky, A. Yagoda, H. Lederman, S. Artzi, M. Gross, Y. Barak, Pregnancy rates are higher with intracytoplasmic morphologically selected sperm injection than with conventional intracytoplasmic injection. Fertil. Steril. 80, 1413–1419 (2003)CrossRef B. Bartoov, A. Berkovitz, F. Eltes, A. Kogosovsky, A. Yagoda, H. Lederman, S. Artzi, M. Gross, Y. Barak, Pregnancy rates are higher with intracytoplasmic morphologically selected sperm injection than with conventional intracytoplasmic injection. Fertil. Steril. 80, 1413–1419 (2003)CrossRef
5.
go back to reference H.E. Chemes, Sperm pathology: a step beyond descriptive morphology. Origin, characterization and fertility potential of abnormal sperm phenotypes in infertile men. Hum. Reprod. Update 9, 405–428 (2003) H.E. Chemes, Sperm pathology: a step beyond descriptive morphology. Origin, characterization and fertility potential of abnormal sperm phenotypes in infertile men. Hum. Reprod. Update 9, 405–428 (2003)
6.
go back to reference J. Tesarik, C. Mendoza, E. Greco, Paternal effects acting during the first cell cycle of human preimplantation development after ICSI. Hum. Reprod. (Oxford, England) 17, 184–189 (2002) J. Tesarik, C. Mendoza, E. Greco, Paternal effects acting during the first cell cycle of human preimplantation development after ICSI. Hum. Reprod. (Oxford, England) 17, 184–189 (2002)
7.
go back to reference A. De Vos, H. Van De Velde, H. Joris, G. Verheyen, P. Devroey, A. Van Steirteghem, Influence of individual sperm morphology on fertilization, embryo morphology, and pregnancy outcome of intracytoplasmic sperm injection. Fertil. Steril. 79, 42–48 (2003)CrossRef A. De Vos, H. Van De Velde, H. Joris, G. Verheyen, P. Devroey, A. Van Steirteghem, Influence of individual sperm morphology on fertilization, embryo morphology, and pregnancy outcome of intracytoplasmic sperm injection. Fertil. Steril. 79, 42–48 (2003)CrossRef
8.
go back to reference G. Huszar, C.C. Ozenci, S. Cayli, Z. Zavaczki, E. Hansch, L. Vigue, Hyaluronic acid binding by human sperm indicates cellular maturity, viability, and unreacted acrosomal status. Fertil. Steril. 79, 1616–1624 (2003)CrossRef G. Huszar, C.C. Ozenci, S. Cayli, Z. Zavaczki, E. Hansch, L. Vigue, Hyaluronic acid binding by human sperm indicates cellular maturity, viability, and unreacted acrosomal status. Fertil. Steril. 79, 1616–1624 (2003)CrossRef
9.
go back to reference A. Jakab, D. Sakkas, E. Delpiano, S. Cayli, E. Kovanci, D. Ward, A. Ravelli, G. Huszar, Intracytoplasmic sperm injection: a novel selection method for sperm with normal frequency of chromosomal aneuploidies. Fertil. Steril. 84, 1665–1673 (2005)CrossRef A. Jakab, D. Sakkas, E. Delpiano, S. Cayli, E. Kovanci, D. Ward, A. Ravelli, G. Huszar, Intracytoplasmic sperm injection: a novel selection method for sperm with normal frequency of chromosomal aneuploidies. Fertil. Steril. 84, 1665–1673 (2005)CrossRef
10.
go back to reference A. Yagci, W. Murk, J. Stronk, G. Huszar, Spermatozoa bound to solid state hyaluronic acid show chromatin structure with high DNA chain integrity: an acridine orange fluorescence study. J. Androl. 31, 566–572 (2010)CrossRef A. Yagci, W. Murk, J. Stronk, G. Huszar, Spermatozoa bound to solid state hyaluronic acid show chromatin structure with high DNA chain integrity: an acridine orange fluorescence study. J. Androl. 31, 566–572 (2010)CrossRef
11.
go back to reference R.P. Amann, D.F. Katz, Reflections on CASA after 25 years. J. Androl. 25, 317–325 (2004) R.P. Amann, D.F. Katz, Reflections on CASA after 25 years. J. Androl. 25, 317–325 (2004)
12.
go back to reference L.Z. Shi, J.M. Nascimento, M.W. Berns, E.L. Botvinick, Computer-based tracking of single sperm. J. Biomed. Optic. 11, 054009 (2006)CrossRef L.Z. Shi, J.M. Nascimento, M.W. Berns, E.L. Botvinick, Computer-based tracking of single sperm. J. Biomed. Optic. 11, 054009 (2006)CrossRef
13.
go back to reference V.R. Nafisi, M.H. Moradi, M.H. Nasr-Esfahani, A template matching algorithm for sperm tracking and classification. Physiol. Meas. 26, 639–651 (2005)CrossRef V.R. Nafisi, M.H. Moradi, M.H. Nasr-Esfahani, A template matching algorithm for sperm tracking and classification. Physiol. Meas. 26, 639–651 (2005)CrossRef
14.
go back to reference C. Leung, Z. Lu, N. Esfandiari, R.F. Casper, Y. Sun, Automated sperm immobilization for intracytoplasmic sperm injection. IEEE Trans. Bio-med. Eng. 58, 935–942 (2011)CrossRef C. Leung, Z. Lu, N. Esfandiari, R.F. Casper, Y. Sun, Automated sperm immobilization for intracytoplasmic sperm injection. IEEE Trans. Bio-med. Eng. 58, 935–942 (2011)CrossRef
15.
go back to reference V.R. Nafisi, M.H. Moradi, M.H. Nasr-esfahani, Sperm identification using elliptic model and tail detection. World Acad. Sci. Eng. Technol. 6, 205–208 (2005) V.R. Nafisi, M.H. Moradi, M.H. Nasr-esfahani, Sperm identification using elliptic model and tail detection. World Acad. Sci. Eng. Technol. 6, 205–208 (2005)
16.
go back to reference A.M. Groenewald, E. Botha, Preprocessing and tracking algorithms for automatic sperm analysis, in COMSIG 1991 Proceedings: South African Symposium on Communications and Signal Processing (IEEE, New York, 1991), pp. 64–68 A.M. Groenewald, E. Botha, Preprocessing and tracking algorithms for automatic sperm analysis, in COMSIG 1991 Proceedings: South African Symposium on Communications and Signal Processing (IEEE, New York, 1991), pp. 64–68
17.
go back to reference L.Z. Shi, J. Nascimento, C. Chandsawangbhuwana, M.W. Berns, E.L. Botvinick, Real-time automated tracking and trapping system for sperm. Microsc. Res. Tech. 69, 894–902 (2006)CrossRef L.Z. Shi, J. Nascimento, C. Chandsawangbhuwana, M.W. Berns, E.L. Botvinick, Real-time automated tracking and trapping system for sperm. Microsc. Res. Tech. 69, 894–902 (2006)CrossRef
18.
go back to reference S. Oehninger, T. Kruger, The diagnosis of male infertility by semen quality. Clinical significance of sperm morphology assessment. Hum. Reprod. (Oxford, England) 10, 1037–1038 (1995) S. Oehninger, T. Kruger, The diagnosis of male infertility by semen quality. Clinical significance of sperm morphology assessment. Hum. Reprod. (Oxford, England) 10, 1037–1038 (1995)
19.
go back to reference Z.P. Nagy, J. Liu, H. Joris, G. Verheyen, H. Tournaye, M. Camus, M.C. Derde, P. Devroey, A.C. Van Steirteghem, The result of intracytoplasmic sperm injection is not related to any of the three basic sperm parameters. Hum. Reprod. (Oxford, England) 10, 1123–1129 (1995) Z.P. Nagy, J. Liu, H. Joris, G. Verheyen, H. Tournaye, M. Camus, M.C. Derde, P. Devroey, A.C. Van Steirteghem, The result of intracytoplasmic sperm injection is not related to any of the three basic sperm parameters. Hum. Reprod. (Oxford, England) 10, 1123–1129 (1995)
20.
go back to reference A. Berkovitz, F. Eltes, S. Yaari, N. Katz, I. Barr, A. Fishman, B. Bartoov, The morphological normalcy of the sperm nucleus and pregnancy rate of intracytoplasmic injection with morphologically selected sperm. Hum. Reprod. (Oxford, England) 20, 185–190 (2005) A. Berkovitz, F. Eltes, S. Yaari, N. Katz, I. Barr, A. Fishman, B. Bartoov, The morphological normalcy of the sperm nucleus and pregnancy rate of intracytoplasmic injection with morphologically selected sperm. Hum. Reprod. (Oxford, England) 20, 185–190 (2005)
21.
go back to reference B. Bartoov, A. Berkovitz, F. Eltes, A. Kogosowski, Y. Menezo, Y. Barak, Real-time fine morphology of motile human sperm cells is associated with IVF-ICSI outcome. J. Androl. 23, 1–8 (2002) B. Bartoov, A. Berkovitz, F. Eltes, A. Kogosowski, Y. Menezo, Y. Barak, Real-time fine morphology of motile human sperm cells is associated with IVF-ICSI outcome. J. Androl. 23, 1–8 (2002)
22.
go back to reference A. Zini, R. Bielecki, D. Phang, M.T. Zenzes, Correlations between two markers of sperm DNA integrity, DNA denaturation and DNA fragmentation, in fertile and infertile men. Fertil. Steril. 75, 674–677 (2001)CrossRef A. Zini, R. Bielecki, D. Phang, M.T. Zenzes, Correlations between two markers of sperm DNA integrity, DNA denaturation and DNA fragmentation, in fertile and infertile men. Fertil. Steril. 75, 674–677 (2001)CrossRef
23.
go back to reference M. Benchaib, V. Braun, J. Lornage, S. Hadj, B. Salle, H. Lejeune, J.F. Guérin, Sperm DNA fragmentation decreases the pregnancy rate in an assisted reproductive technique. Hum. Reprod. (Oxford, England) 18, 1023–1028 (2003) M. Benchaib, V. Braun, J. Lornage, S. Hadj, B. Salle, H. Lejeune, J.F. Guérin, Sperm DNA fragmentation decreases the pregnancy rate in an assisted reproductive technique. Hum. Reprod. (Oxford, England) 18, 1023–1028 (2003)
24.
go back to reference M. Sergerie, G. Laforest, L. Bujan, F. Bissonnette, G. Bleau, Sperm DNA fragmentation: threshold value in male fertility. Hum. Reprod. (Oxford, England), 20, 3446–3451 (2005) M. Sergerie, G. Laforest, L. Bujan, F. Bissonnette, G. Bleau, Sperm DNA fragmentation: threshold value in male fertility. Hum. Reprod. (Oxford, England), 20, 3446–3451 (2005)
25.
go back to reference W. Gorczyca, F. Traganos, H. Jesionowska, Z. Darzynkiewicz, Presence of DNA strand breaks and increased sensitivity of DNA in situ to denaturation in abnormal human sperm cells: analogy to apoptosis of somatic cells. Exp. Cell Res. 207, 202–205 (1993)CrossRef W. Gorczyca, F. Traganos, H. Jesionowska, Z. Darzynkiewicz, Presence of DNA strand breaks and increased sensitivity of DNA in situ to denaturation in abnormal human sperm cells: analogy to apoptosis of somatic cells. Exp. Cell Res. 207, 202–205 (1993)CrossRef
26.
go back to reference C.M. Hughes, S.E. Lewis, V.J. McKelvey-Martin, W. Thompson, A comparison of baseline and induced DNA damage in human spermatozoa from fertile and infertile men, using a modified comet assay. Mol. Hum. Reprod. 2, 613–619 (1996)CrossRef C.M. Hughes, S.E. Lewis, V.J. McKelvey-Martin, W. Thompson, A comparison of baseline and induced DNA damage in human spermatozoa from fertile and infertile men, using a modified comet assay. Mol. Hum. Reprod. 2, 613–619 (1996)CrossRef
27.
go back to reference M.J. Tomlinson, O. Moffatt, G.C. Manicardi, D. Bizzaro, M. Afnan, D. Sakkas, Interrelationships between seminal parameters and sperm nuclear DNA damage before and after density gradient centrifugation: implications for assisted conception. Hum. Reprod. (Oxford, England) 16, 2160–2165 (2001) M.J. Tomlinson, O. Moffatt, G.C. Manicardi, D. Bizzaro, M. Afnan, D. Sakkas, Interrelationships between seminal parameters and sperm nuclear DNA damage before and after density gradient centrifugation: implications for assisted conception. Hum. Reprod. (Oxford, England) 16, 2160–2165 (2001)
28.
go back to reference J.L. Fernández, F. Vázquez-Gundín, A. Delgado, V.J. Goyanes, J. Ramiro-Díaz, J. de la Torre, J. Gosálvez, DNA breakage detection-FISH (DBD-FISH) in human spermatozoa: technical variants evidence different structural features. Mutat. Res. 453, 77–82 (2000)CrossRef J.L. Fernández, F. Vázquez-Gundín, A. Delgado, V.J. Goyanes, J. Ramiro-Díaz, J. de la Torre, J. Gosálvez, DNA breakage detection-FISH (DBD-FISH) in human spermatozoa: technical variants evidence different structural features. Mutat. Res. 453, 77–82 (2000)CrossRef
29.
go back to reference J.L. Fernández, L. Muriel, M.T. Rivero, V. Goyanes, R. Vazquez, J.G. Alvarez, The sperm chromatin dispersion test: a simple method for the determination of sperm DNA fragmentation. J. Androl. 24, 59–66 (2003) J.L. Fernández, L. Muriel, M.T. Rivero, V. Goyanes, R. Vazquez, J.G. Alvarez, The sperm chromatin dispersion test: a simple method for the determination of sperm DNA fragmentation. J. Androl. 24, 59–66 (2003)
30.
go back to reference D.P. Evenson, Z. Darzynkiewicz, M.R. Melamed, Relation of mammalian sperm chromatin heterogeneity to fertility. Science (New York, NY) 210, 1131–1133 (1980) D.P. Evenson, Z. Darzynkiewicz, M.R. Melamed, Relation of mammalian sperm chromatin heterogeneity to fertility. Science (New York, NY) 210, 1131–1133 (1980)
31.
go back to reference D.P. Evenson, R. Wixon, Comparison of the Halosperm test kit with the sperm chromatin structure assay (SCSA) infertility test in relation to patient diagnosis and prognosis. Fertil. Steril. 84, 846–849 (2005)CrossRef D.P. Evenson, R. Wixon, Comparison of the Halosperm test kit with the sperm chromatin structure assay (SCSA) infertility test in relation to patient diagnosis and prognosis. Fertil. Steril. 84, 846–849 (2005)CrossRef
32.
go back to reference D. Sakkas, J.G. Alvarez, Sperm DNA fragmentation: mechanisms of origin, impact on reproductive outcome, and analysis. Fertil. Steril. 93, 1027–1036 (2010)CrossRef D. Sakkas, J.G. Alvarez, Sperm DNA fragmentation: mechanisms of origin, impact on reproductive outcome, and analysis. Fertil. Steril. 93, 1027–1036 (2010)CrossRef
33.
go back to reference C.G. Petersen, F.C. Massaro, A.L. Mauri, J.B. Oliveira, R.L. Baruffi, J.G. Franco, Efficacy of hyaluronic acid binding assay in selecting motile spermatozoa with normal morphology at high magnification. Reprod. Biol. Endocrinol.: RB&E 8, 149 (2010) C.G. Petersen, F.C. Massaro, A.L. Mauri, J.B. Oliveira, R.L. Baruffi, J.G. Franco, Efficacy of hyaluronic acid binding assay in selecting motile spermatozoa with normal morphology at high magnification. Reprod. Biol. Endocrinol.: RB&E 8, 149 (2010)
34.
go back to reference G.D. Palermo, P.N. Schlegel, L.T. Colombero, N. Zaninovic, F. Moy, Z. Rosenwaks, Aggressive sperm immobilization prior to intracytoplasmic sperm injection with immature spermatozoa improves fertilization and pregnancy rates. Hum. Reprod. (Oxford, England) 11, 1023–1029 (1996) G.D. Palermo, P.N. Schlegel, L.T. Colombero, N. Zaninovic, F. Moy, Z. Rosenwaks, Aggressive sperm immobilization prior to intracytoplasmic sperm injection with immature spermatozoa improves fertilization and pregnancy rates. Hum. Reprod. (Oxford, England) 11, 1023–1029 (1996)
35.
go back to reference Y. Sun, S. Duthaler, B.J. Nelson, Autofocusing in computer microscopy: selecting the optimal focus algorithm. Microsc. Res. Tech. 65, 139–149 (2004)CrossRef Y. Sun, S. Duthaler, B.J. Nelson, Autofocusing in computer microscopy: selecting the optimal focus algorithm. Microsc. Res. Tech. 65, 139–149 (2004)CrossRef
36.
go back to reference X. Zhang, C. Leung, Z. Lu, N. Esfandiari, R.F. Casper, Y. Sun, Controlled aspiration and positioning of biological cells in a micropipette. IEEE Trans. Bio-med. Eng. 59, 1032–1040 (2012)CrossRef X. Zhang, C. Leung, Z. Lu, N. Esfandiari, R.F. Casper, Y. Sun, Controlled aspiration and positioning of biological cells in a micropipette. IEEE Trans. Bio-med. Eng. 59, 1032–1040 (2012)CrossRef
Metadata
Title
Human Sperm Tracking, Analysis, and Manipulation
Authors
Jun Liu
Clement Leung
Zhe Lu
Yu Sun
Copyright Year
2013
Publisher
Springer New York
DOI
https://doi.org/10.1007/978-1-4614-6684-0_12