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06-01-2021 | Original Research

Impacts of cotton linter pulp characteristics on the processivity of glycoside hydrolase family 5 endoglucanase from Volvariella Volvacea

Authors: Shanshan Wu, Xiao Jiang, Huicong Jiang, Shufang Wu, Shaojun Ding, Yongcan Jin

Published in: Cellulose | Issue 4/2021

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Abstract

EG1 from Volvariella volvacea is a processive endoglucanase belonging to glycoside hydrolase family 5. The impacts of cotton linter pulp characteristics, such as degree of polymerization (DP), crystallinity, and the initial cellulose-reducing ends, on the processivity of EG1 were investigated. Three commercial cotton linter pulp with different DP were used in present study. Ball milling was used to alter the crystallinity and DP of cellulose. The results indicate that the crystallinity has the most significant impact on enzyme processivity followed by initial cellulose-reducing ends. Whereas the DP indirectly affects the enzymatic hydrolysis and influenced by the pulp preparation method and conditions. The initial cellulose-reducing ends also affect enzyme adsorption but their impact is not obvious when the crystallinity is very low. These results also demonstrate the endo- and exo-action are both exist for EG1. The processive exo-action can start from the newly created cellulose-reducing ends by endo-action as well as the initial cellulose-reducing ends. The contribution of initial cellulose-reducing ends is affected by its quantity and cellulose crystallinity. A plausible action mode for EG1 is also proposed.

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Asha P, Divya J, Bright Singh IS (2016) Purification and characterisation of processive-type endoglucanase and β-glucosidase from Aspergillus ochraceus MTCC 1810 through saccharification of delignified coir pith to glucose. Bioresoure Technol 213:245–248. https://doi.org/10.1016/j.biortech.2016.03.013CrossRef

Boraston AB, Bolam DN, Gilbert HJ, Davies GJ (2004) Carbohydrate-binding modules: fine-tuning polysaccharide recognition. Biochem J 382:769–781. https://doi.org/10.1042/BJ20040892CrossRefPubMedPubMedCentral

Chiriac AI, Cadena EM, Vidal T, Torres AL, Diaz P, Javier Pastor FI (2010) Engineering a family 9 processive endoglucanase from Paenibacillus barcinonensis displaying a novel architecture. Appl Microbiol Biot 86:1125–1134. https://doi.org/10.1007/s00253-009-2350-8CrossRef

Chiriac AI, Pastor FIJ, Popa VI, Aflori M, Ciolacu D (2014) Changes of supramolecular cellulose structure and accessibility induced by the processive endoglucanase Cel9B from Paenibacillus barcinonensis. Cellulose 21:203–219. https://doi.org/10.1007/s10570-013-0118-xCrossRef

Davies G, Henrissat B (1995) Structures and mechanism of glycosyl hydrolases. Structure 3:853–859CrossRef

Ding SJ, Ge W, Buswell JA (2001) Endoglucanase I from the edible straw mushroom, Volvariella volvacea. Eur J Biochem 268:5687–5695. https://doi.org/10.1046/j.0014-2956.2001.02503.xCrossRefPubMed

French AD, Kim HJ (2018) Cotton fiber: physics, chemistry and biology. Springer, Brlin, pp 13–39CrossRef

Ghatge SS et al (2014) Characterization of modular bifunctional processive endoglucanase Cel5 from Hahella chejuensis KCTC 2396. Appl Microbiol Biot 98:4421–4435. https://doi.org/10.1007/s00253-013-5446-0CrossRef

Horn SJ et al (2006) Costs and benefits of processivity in enzymatic degradation of recalcitrant polysaccharides. Proc Natl Acad Sci USA 103:18089–18094. https://doi.org/10.1073/pnas.0608909103CrossRefPubMed

Irwin DC, Spezio M, Walker LP, Wilson DB (2010) Activity studies of eight purified cellulases: Specificity, synergism, and binding domain effects. Biotechnology Bioengineering 42:1002–1013. https://doi.org/10.1002/bit.260420811CrossRef

Ji G, Gao C, Xiao W, Han L (2016) Mechanical fragmentation of corncob at different plant scales: Impact and mechanism on microstructure features and enzymatic hydrolysis. Bioresource Technol 205:159–165. https://doi.org/10.1016/j.biortech.2016.01.029CrossRef

Jin E, Guo J, Yang F, Zhu Y, Song J, Jin Y, Rojas OJ (2016) On the polymorphic and morphological changes of cellulose nanocrystals (CNC-I) upon mercerization and conversion to CNC-II. Carbohydr Polym 143:327–335. https://doi.org/10.1016/j.carbpol.2016.01.048CrossRefPubMed

Kim HM, Lee YG, Patel DH, Lee KH, Lee DS, Bae HJ (2012) Characteristics of bifunctional acidic endoglucanase (Cel5B) from Gloeophyllum trabeum. Journal Industrial Microbiology Biotechnology 39:1081–1089. https://doi.org/10.1007/s10295-012-1110-4CrossRefPubMed

Kongruang S, Penner MH (2004) Borohydride reactivity of cellulose reducing ends. Carbohyd Polym 58:131–138. https://doi.org/10.1016/j.carbpol.2004.06.012CrossRef

Kostylev M, Moran Mirabal JM, Walker LP, Wilson DB (2012) Determination of the molecular states of the processive endocellulase Thermobifida fusca Cel9A during crystalline cellulose depolymerization. Biotechnol Bioeng 109:295–299. https://doi.org/10.1002/bit.23299CrossRefPubMed

Li X, Zheng Y (2017) Lignin-enzyme interaction: Mechanism, mitigation approach, modeling, and research prospects. Biotechnol Adv 35:466–489. https://doi.org/10.1016/j.biotechadv.2017.03.010CrossRefPubMed

Li Y, Irwin DC, Wilson DB (2007) Processivity, substrate binding, and mechanism of cellulose hydrolysis by Thermobifida fusca Cel9A. Appl Environ Microb 73:3165–3172. https://doi.org/10.1128/AEM.02960-06CrossRef

Linder M et al (1995) Identification of functionally important amino acids in the cellulose-binding domain of Trichoderma reesei cellobiohydrolase I. Protein Sci 4:1056–1064. https://doi.org/10.1002/pro.5560040604CrossRefPubMedPubMedCentral

Ling Z et al (2019) Effects of ball milling on the structure of cotton cellulose. Cellulose 26:305–328. https://doi.org/10.1007/s10570-018-02230-xCrossRef

Lu M, Li J, Han L, Xiao W (2019) An aggregated understanding of cellulase adsorption and hydrolysis for ball-milled cellulose. Bioresource Technol 273:1–7. https://doi.org/10.1016/j.biortech.2018.10.037CrossRef

Nimlos MR, Beckham GT, Matthews JF, Bu L, Himmel ME, Crowley MF (2012) Binding preferences, surface attachment, diffusivity, and orientation of a family 1 carbohydrate-binding module on cellulose. J Biol Chem 287:20603–20612. https://doi.org/10.1074/jbc.M112.358184CrossRefPubMedPubMedCentral

O Dell PJ, Mudinoor AR, Parikh SJ, Jeoh T (2015) The effect of fibril length and architecture on the accessibility of reducing ends of cellulose Iα to Trichoderma reesei Cel7A. Cellulose 22:1697–1713. https://doi.org/10.1007/s10570-015-0618-yCrossRef

Obeng EM, Adam SNN, Budiman C, Ongkudon CM, Maas R, Jose J (2017) Lignocellulases: a review of emerging and developing enzymes, systems, and practices. Bioresources Bioprocess 4:16. https://doi.org/10.1186/s40643-017-0146-8CrossRef

Oliveira OV, Freitas LCG, Straatsma TP, Lins RD (2009) Interaction between the CBM of Cel9A from Thermobifida fusca and cellulose fibers. J Mol Recognit 22:38–45. https://doi.org/10.1002/jmr.925CrossRefPubMed

Praestgaard E, Elmerdahl J, Murphy L, Nymand SR, Mcfarland KC, Borch K, Westh P (2011) A kinetic model for the burst phase of processive cellulases. Febs J 278:1547–1560. https://doi.org/10.1111/j.1742-4658.2011.08078.xCrossRefPubMed

Sakon J, Irwin D, Wilson DB, Karplus PA (1997) Structure and mechanism of endo/exocellulase E4 from Thermomonospora fusca. Nat Struct Biol 4:810–818. https://doi.org/10.1038/nsb1097-810CrossRefPubMed

Schüth F, Rinaldi R, Meine N, Käldström M, Hilgert J, Rechulski K (2014) Mechanocatalytic depolymerization of cellulose and raw biomass and downstream processing of the products. Catal Today 234:24–30. https://doi.org/10.1016/j.cattod.2014.02.019CrossRef

Sinitsyn AP, Gusakov AV, Vlasenko EY (1991) Effect of structural and physico-chemical features of cellulosic substrates on the efficiency of enzymatic hydrolysis. Applied Biochemistry Biotechnology 30:43–59. https://doi.org/10.1007/BF02922023CrossRef

Sipponen MH et al (2017) Structural changes of lignin in biorefinery pretreatments and consequences to enzyme-lignin interactions. Nordic Pulp Paper Res J 32:550–571. https://doi.org/10.3183/NPPRJ-2017-32-04-p550-571CrossRef

Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D, Crocker D (2008) Determination of structural carbohydrates and lignin in biomass. Lab Anal Procedure 1617:1–16

Smith PK et al (1985) Measurement of protein using bicinchoninic acid. Anal Biochem 150:76–85. https://doi.org/10.1016/0003-2697(85)90442-7CrossRefPubMed

Somogyi M (1952) Notes on sugar determination. J Biol Chem 195:19–23. https://doi.org/10.1515/bchm2.1952.289.5-6.309CrossRef

Sulzenbacher G, Schülein M, Davies GJ (1997) Structure of the endoglucanase I from Fusarium oxysporum: Native, cellobiose, and 3,4-epoxybutyl β-d-cellobioside-inhibited Forms, at 2.3 Å resolution. Biochemistry-Us 36:5902–5911. https://doi.org/10.1021/bi962963+CrossRef

Taylor LE, Bernard H, Coutinho PM, Ekborg NA, Hutcheson SW, Weiner RM (2006) Complete cellulase system in the marine bacterium Saccharophagus degradans strain 2-40T. J Bacteriol 188:3849–3861. https://doi.org/10.1128/jb.01348-05CrossRefPubMedPubMedCentral

Velleste R, Teugjas H, Väljamäe P (2010) Reducing end-specific fluorescence labeled celluloses for cellulase mode of action. Cellulose 17:125–138. https://doi.org/10.1007/s10570-009-9356-3CrossRef

von Hippel HP (1994) Protein-DNA recognition: new perspectives and underlying themes. Science 263:769–770. https://doi.org/10.1126/science.8303292CrossRef

Watson BJ, Zhang H, Longmire AG, Moon YH, Hutcheson SW (2009) Processive endoglucanases mediate degradation of cellulose by Saccharophagus degradans. J Bacteriol 191:5697–5705. https://doi.org/10.1128/JB.00481-09CrossRefPubMedPubMedCentral

Wu B, Zheng S, Pedroso MM, Guddat LW, Chang S, He B, Schenk G (2018) Processivity and enzymatic mechanism of a multifunctional family 5 endoglucanase from Bacillus subtilis BS-5 with potential applications in the saccharification of cellulosic substrates. Biotechnol Biofuels 11:20. https://doi.org/10.1186/s13068-018-1022-2CrossRefPubMedPubMedCentral

Wu S, Wu S (2019) Processivity and the mechanisms of processive endoglucanases. Appl Biochem Biotech 190(2):448–463. https://doi.org/10.1007/s12010-019-03096-wCrossRef

Yang B, Dai Z, Ding SY, Wyman CE (2011) Enzymatic hydrolysis of cellulosic biomass. Biofuels 2:421–450. https://doi.org/10.4155/bfs.11.116CrossRef

Zhang K et al (2018) Processive degradation of crystalline cellulose by a multimodular endoglucanase via a wirewalking mode. Biomacromol 19:1686–1696. https://doi.org/10.1021/acs.biomac.8b00340CrossRef

Zhang Y, Jiang X, Wan S, Wu W, Wu S, Jin Y (2020) Adsorption behavior of two glucanases on three lignins and the effect by adding sulfonated lignin. J Biotechnol 323:1–8. https://doi.org/10.1016/j.jbiotec.2020.07.013CrossRefPubMed

Zhang YHP, Lynd LR (2005) Determination of the number-average degree of polymerization of cellodextrins and cellulose with application to enzymatic hydrolysis. Biomacromol 6:1510–1515. https://doi.org/10.1021/bm049235jCrossRef

Zheng F, Ding S (2013) Processivity and enzymatic mode of a glycoside hydrolase family 5 endoglucanase from Volvariella volvacea. Appl Environ Microb 79:989–996. https://doi.org/10.1128/AEM.02725-12CrossRef

Title: Impacts of cotton linter pulp characteristics on the processivity of glycoside hydrolase family 5 endoglucanase from Volvariella Volvacea
Authors: Shanshan Wu
Xiao Jiang
Huicong Jiang
Shufang Wu
Shaojun Ding
Yongcan Jin
Publication date: 06-01-2021
Publisher: Springer Netherlands
Published in: Cellulose / Issue 4/2021
Print ISSN: 0969-0239
Electronic ISSN: 1572-882X
DOI: https://doi.org/10.1007/s10570-020-03665-x

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