In vitro and in vivo Examinations for Detection of Minimal Infective Dose for Biomaterials

The objective of the study is to examine the minimal infective dose of

Staphylococcus epidermidis

and

Pseudomonas aeruginosa

on biomaterials in vivo and in vitro. In this research originally synthesized bioactive biomaterials – 4N glass and 4NK glass-ceramics are calcium phosphate with Na

2

O and Nb

2

O

5

additives with different crystallinity. For microbiological investigation of biomaterials

S.epidermidis

strain ATCC 12228,

Ps.aeruginosa

strain ATCC 27853, 4 rabbit animal models, broth and agar, scanned electron microscope Tescan,

Vortex

centrifuge, sonicator and histological methods were used. We observed that different biomaterials had various attachments of

S.epidermidis

and

Ps.aeruginosa

in laboratory animal models. The minimal infective dose of

S.epidermidis

for biomaterial 4NK is more than 10

3

CFU/ml, for biomaterial 4N less than 10

3

CFU/ml. The minimal infective dose of

Ps.aeruginosa

for biomaterial 4N and biomaterial 4NK is less than 10

3

CFU/ml.