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Journal of Materials Engineering and Performance

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02-05-2016

Influence of Fe₃O₄ Nanoparticles in Hydroxyapatite Scaffolds on Proliferation of Primary Human Fibroblast Cells

Authors: H. Maleki-Ghaleh, E. Aghaie, A. Nadernezhad, M. Zargarzadeh, A. Khakzad, M. S. Shakeri, Y. Beygi Khosrowshahi, M. H. Siadati

Published in: Journal of Materials Engineering and Performance | Issue 6/2016

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Abstract

Modern techniques for expanding stem cells play a substantial role in tissue engineering: the raw material that facilitates regeneration of damaged tissues and treats diseases. The environmental conditions and bioprocessing methods are the primary determinants of the rate of cultured stem cell proliferation. Bioceramic scaffolds made of calcium phosphate are effective substrates for optimal cell proliferation. The present study investigates the effects of two bioceramic scaffolds on proliferating cells in culture media. One scaffold was made of hydroxyapatite and the other was a mixture of hydroxyapatite and ferromagnetic material (Fe₃O₄ nanoparticles). Disk-shaped (10 mm × 2 mm) samples of the two scaffolds were prepared. Primary human fibroblast proliferation was 1.8- and 2.5-fold faster, respectively, when cultured in the presence of hydroxyapatite or ferrous nanoparticle/hydroxyapatite mixtures. Optical microscopy images revealed that the increased proliferation was due to enhanced cell-cell contact. The presence of magnetic Fe₃O₄ nanoparticles in the ceramic scaffolds significantly increased cell proliferation compared to hydroxyapatite scaffolds and tissue culture polystyrene.

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Title: Influence of Fe3O4 Nanoparticles in Hydroxyapatite Scaffolds on Proliferation of Primary Human Fibroblast Cells
Authors: H. Maleki-Ghaleh
E. Aghaie
A. Nadernezhad
M. Zargarzadeh
A. Khakzad
M. S. Shakeri
Y. Beygi Khosrowshahi
M. H. Siadati
Publication date: 02-05-2016
Publisher: Springer US
Published in: Journal of Materials Engineering and Performance / Issue 6/2016
Print ISSN: 1059-9495
Electronic ISSN: 1544-1024
DOI: https://doi.org/10.1007/s11665-016-2086-4

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