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15-02-2023

Inhibitory effect of volatile organic compounds from Bacillus flexus TR-1 against Aspergillus flavus and aflatoxins in grains during storage

Authors: An-Dong Gong, Meng-Ge Song, Hua-Ling Wang, Gao-Zhan Wang, Jian-Hua Wang, Jing-Bo Zhang

Published in: BioControl | Issue 2/2023

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Abstract

Aspergillus flavus infects stored grains and produces aflatoxins with highly toxic and carcinogenic properties, which are a threat to food safety and human health. Microbes and their bio-active compounds are considered effective agents to control A. flavus and aflatoxins during the pre- and post-harvest stages. Here, we reveal that Bacillus flexus strain TR-1 isolated from tea plant rhizosphere soil could efficiently inhibit the growth of A. flavus in dual culture tests through the production of antifungal volatiles. Additionally, these volatiles showed great inhibitory effects against A. flavus infection and aflatoxin production in maize and peanut with high water activity levels during storage. Scanning electron microscope analysis further proved that the conidia of A. flavus on the peanut surface treated with TR-1 volatiles could not germinate and were severely dehydrated and shrunken. Gas chromatography-tandem mass spectrometry revealed that five antifungal compounds were identified in the volatile profiles, including benzene, methyl thiolacetate, dimethyl disulfide, butanethioic acid S-methyl ester analogue, dimethyl trisulfide (DMTS), and S-methyl 3-methylbutanethioate. DMTS, with the best antagonistic effect, completely inhibited the growth of A. flavus at 10 μl−1 (compound volume/airspace volume). TR-1 also exhibited efficient and broad inhibitory effects against six other fungal pathogens. Overall, B. flexus strain TR-1 and its produced volatiles showed valid antifungal activity against phytopathogens. This study provides novel materials for the production of bioactive agents to control plant pathogens in stored grains.

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Appendix
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Metadata
Title
Inhibitory effect of volatile organic compounds from Bacillus flexus TR-1 against Aspergillus flavus and aflatoxins in grains during storage
Authors
An-Dong Gong
Meng-Ge Song
Hua-Ling Wang
Gao-Zhan Wang
Jian-Hua Wang
Jing-Bo Zhang
Publication date
15-02-2023
Publisher
Springer Netherlands
Published in
BioControl / Issue 2/2023
Print ISSN: 1386-6141
Electronic ISSN: 1573-8248
DOI
https://doi.org/10.1007/s10526-023-10187-5

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