Investigation of In Situ Systems of Progesterone in Biphasic Media

Livestock breeding is one of the main concerns for animal owners. Progesterone plays a crucial role in regulating the activity of the reproductive system. In the present study, in situ formed systems that include poly(lactic-co-glycolic) acid (PLGA) as a biodegradable polymer, N-methyl-2-pyrrolidone (NMP) as a bioavailable solvent, and sodium chloride (NaCl) as an additive were provided to treat abortion. The release media was selected as a biphasic that contain 1,2-dichloroethane and buffer phosphate (87:13%) at 37 °C. The high-performance liquid chromatography system was also used to determine the amount of drug release. Degradation of systems was determined by examining the process of pH change. In general, it can be concluded that in situ systems based on PLGA containing NMP with 30 wt% sodium chloride salts showed a linear release profile.