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2014 | OriginalPaper | Chapter

109. Isolation and Characterization of a New Moderately Halophilic Bacterium Strain SM. 200-5 from Solar Saltern Ponds

Authors : Gaochao Xu, Yuangao Deng, Donghui Song, Liying Sui

Published in: Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)

Publisher: Springer Berlin Heidelberg

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Abstract

A new moderately halophilic bacterium strain SM. 200-5 was isolated from the solar saltern ponds with salinity 200 in Hangu Saltworks, Tianjin, China. The cells of the strain SM. 200-5 were rods and Gram-negative. They could grow in a salinity range of 30–150 and initial pH 6–11, with optimum of salinity 100 and initial pH 7. 16S rDNA alignment showed that the strain SM. 200-5 had 93–95 % similarity with those sequences of genus Salimicrobium sp.. Biochemical characterization analysis via API 20E system indicated that biochemical characters of the strain SM. 200-5 were partially different from Salimicrobium sp. ISL-25, which was its closest member in the phylogenetic tree. Therefore, the strain SM. 200-5 proposed a new species of Salimicrobium.

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Literature
1.
go back to reference Oren A, Larimer F, Richardson P et al (2005) How to be moderately halophilic with broad salt tolerance: clues from the genome of Chromohalobacter salexigens. Extremophiles 4:275–279CrossRef Oren A, Larimer F, Richardson P et al (2005) How to be moderately halophilic with broad salt tolerance: clues from the genome of Chromohalobacter salexigens. Extremophiles 4:275–279CrossRef
2.
go back to reference Ashrafaddin S, Ali AK, Ferdous RJ (2005) Identification and characterization of salt-inducible polypeptide in Paenibacillus sp., a moderately halophilic bacterium. J Biosci Bioeng 5:573–575 Ashrafaddin S, Ali AK, Ferdous RJ (2005) Identification and characterization of salt-inducible polypeptide in Paenibacillus sp., a moderately halophilic bacterium. J Biosci Bioeng 5:573–575
3.
go back to reference Rodríguez-Valera F, Ventosa A, Juez G (1985) Variation of environmental features and microbial populations with salt concentrations in a multi-pond saltern. Microb Ecol 2:107–115CrossRef Rodríguez-Valera F, Ventosa A, Juez G (1985) Variation of environmental features and microbial populations with salt concentrations in a multi-pond saltern. Microb Ecol 2:107–115CrossRef
4.
go back to reference Oren A (2008) Microbial life at high salt concentrations: phylogenetic and metabolic diversity. Saline Syst 1:2–14CrossRef Oren A (2008) Microbial life at high salt concentrations: phylogenetic and metabolic diversity. Saline Syst 1:2–14CrossRef
5.
go back to reference Liu JJ, Chen QZ, Zeng JN et al (2007) Advances in the research of bioactive substances from marine microorganisms. J Mar Sci 1:55–65 (Chinese) Liu JJ, Chen QZ, Zeng JN et al (2007) Advances in the research of bioactive substances from marine microorganisms. J Mar Sci 1:55–65 (Chinese)
6.
go back to reference Lv JS, Huang HQ, Cong M et al (2006) Identification for a marine bacterium and preliminary study on purification of metabolite from the bacterium. Acta oceanol sin 5:173–178 Lv JS, Huang HQ, Cong M et al (2006) Identification for a marine bacterium and preliminary study on purification of metabolite from the bacterium. Acta oceanol sin 5:173–178
7.
go back to reference Oren A (2002) Diversity of halophilic microorganisms: environments, phylogeny, physiology, and applications. J Ind Microbiol Biotechnol 1:56–63 Oren A (2002) Diversity of halophilic microorganisms: environments, phylogeny, physiology, and applications. J Ind Microbiol Biotechnol 1:56–63
8.
go back to reference Boudjelal F, Zitouni A, Mathieu F et al (2011) Taxonomy and antimicrobial activities of two novel halophilic Saccharomonospora strains isolated in Algerian Sahara soils. Ann Microbiol 2:299–305CrossRef Boudjelal F, Zitouni A, Mathieu F et al (2011) Taxonomy and antimicrobial activities of two novel halophilic Saccharomonospora strains isolated in Algerian Sahara soils. Ann Microbiol 2:299–305CrossRef
9.
go back to reference Das Sarma P, Das Sarma S (2008) On the origin of prokaryotic “species”: the taxonomy of halophilic Archaea. Saline Syst 4:5–9CrossRef Das Sarma P, Das Sarma S (2008) On the origin of prokaryotic “species”: the taxonomy of halophilic Archaea. Saline Syst 4:5–9CrossRef
10.
go back to reference Wang J, Dang HY, Yang GP et al (2009) Physiochemical and phylogenetic analysis of moderately halophilic bacteria halomonadaceae in deep-sea sediments of the Southern Okinawa Trough. J Mar Sci 5:17–22 (Chinese) Wang J, Dang HY, Yang GP et al (2009) Physiochemical and phylogenetic analysis of moderately halophilic bacteria halomonadaceae in deep-sea sediments of the Southern Okinawa Trough. J Mar Sci 5:17–22 (Chinese)
11.
go back to reference Tang J, Zheng AP, Bromfield ESP et al (2011) 16S rRNA gene sequence analysis of halophilic and halo-tolerant bacteria isolated from a hypersaline pond in Sichuan, China. Ann Microbiol 2:375–381CrossRef Tang J, Zheng AP, Bromfield ESP et al (2011) 16S rRNA gene sequence analysis of halophilic and halo-tolerant bacteria isolated from a hypersaline pond in Sichuan, China. Ann Microbiol 2:375–381CrossRef
12.
go back to reference Yeon SH, Jeong WJ, Park JS (2005) The diversity of culturable organotrophic bacteria from local solar salterns. J Microbiol 1:1–10 Yeon SH, Jeong WJ, Park JS (2005) The diversity of culturable organotrophic bacteria from local solar salterns. J Microbiol 1:1–10
13.
go back to reference Zahran HH (1997) Diversity, adaptation and activity of the bacterial flora in saline environments. Biol Fert 3:211–223CrossRef Zahran HH (1997) Diversity, adaptation and activity of the bacterial flora in saline environments. Biol Fert 3:211–223CrossRef
14.
go back to reference Brown LS (2001) Proton transport mechanism of bacteriorhodopsin as revealed by site-specific mutagenesis and protein sequence variability. Biochemistry 11:1249–1255 Brown LS (2001) Proton transport mechanism of bacteriorhodopsin as revealed by site-specific mutagenesis and protein sequence variability. Biochemistry 11:1249–1255
15.
go back to reference Habden X, Tohty D, Wu M et al (2007) Studies on bacteriorhodopsin gene and sequence of 16S rRNA encoding genes of halophilic archaea of Xinjiang Aibi Lake. J Biotechnol 1:46–50 (Chinese) Habden X, Tohty D, Wu M et al (2007) Studies on bacteriorhodopsin gene and sequence of 16S rRNA encoding genes of halophilic archaea of Xinjiang Aibi Lake. J Biotechnol 1:46–50 (Chinese)
16.
go back to reference Lanyi JK, Schobert B (2003) Mechanism of proton transport in bacteriorhodopsin from crystallographic structures of the K, L, M1, M2, and M2’ intermediates of the photocycle. J Mol Biol 2:439–450CrossRef Lanyi JK, Schobert B (2003) Mechanism of proton transport in bacteriorhodopsin from crystallographic structures of the K, L, M1, M2, and M2’ intermediates of the photocycle. J Mol Biol 2:439–450CrossRef
17.
go back to reference Kis-Papo T, Oren A (2000) Halocins: are they involved in the competition between halobacteria in saltern ponds. Extremophiles 1:35–41 Kis-Papo T, Oren A (2000) Halocins: are they involved in the competition between halobacteria in saltern ponds. Extremophiles 1:35–41
18.
go back to reference Guo JB, Zhou JT, Wang D et al (2008) A novel moderately halophilic bacterium for decolorizing azo dye under high salt condition. Biodegradation 1:15–19CrossRef Guo JB, Zhou JT, Wang D et al (2008) A novel moderately halophilic bacterium for decolorizing azo dye under high salt condition. Biodegradation 1:15–19CrossRef
19.
go back to reference Litchfield CD (2011) Potential for industrial products from the halophilic Archaea. J Mol Biol 10:1635–1647 Litchfield CD (2011) Potential for industrial products from the halophilic Archaea. J Mol Biol 10:1635–1647
20.
go back to reference Sorgeloos P, Takaert W (1990) Roles and potentials of Artemia in coastal saltworks. In: Cheng L (ed) Proceeding of International Symposium on Biotechnology of Saltponds, Tanggu, Tianjin, pp 69–77 Sorgeloos P, Takaert W (1990) Roles and potentials of Artemia in coastal saltworks. In: Cheng L (ed) Proceeding of International Symposium on Biotechnology of Saltponds, Tanggu, Tianjin, pp 69–77
21.
go back to reference Yoon JH, Kang KH, Oh TK et al (2004) Halobacillus locisalis sp. nov., a halophilic bacterium isolated from a marine solar saltern of the Yellow Sea in Korea. Extremophiles 1:23–28CrossRef Yoon JH, Kang KH, Oh TK et al (2004) Halobacillus locisalis sp. nov., a halophilic bacterium isolated from a marine solar saltern of the Yellow Sea in Korea. Extremophiles 1:23–28CrossRef
22.
go back to reference Montalvo-Rodríguez R, Ruíz-acevedo A, Ló pez-Garriga J (1997) New isolates of extremely halophilic Archaebacteria (Halobacteria) from Puerto Rico and the Caribbean. Caribean J Sci 1:98–104 Montalvo-Rodríguez R, Ruíz-acevedo A, Ló pez-Garriga J (1997) New isolates of extremely halophilic Archaebacteria (Halobacteria) from Puerto Rico and the Caribbean. Caribean J Sci 1:98–104
23.
go back to reference Chen L, Wang GY, Bu T et al (2010) Identification of a moderately halophilic bacterium whb45 and screening of its antimicrobial and antitumor activity. Microbiol China 1:85– 90 (Chinese) Chen L, Wang GY, Bu T et al (2010) Identification of a moderately halophilic bacterium whb45 and screening of its antimicrobial and antitumor activity. Microbiol China 1:85– 90 (Chinese)
24.
go back to reference Gochnauer MB, Kushner DJ (1969) Growth and nutrition of extremely halophilic bacteria. Canadian J Microbiol 10:1157–1165CrossRef Gochnauer MB, Kushner DJ (1969) Growth and nutrition of extremely halophilic bacteria. Canadian J Microbiol 10:1157–1165CrossRef
25.
go back to reference Sehgal SN, Gibbons NE (1960) Effect of some metal ions on the growth of halobacterium cutirubrum. Canadian J Microbiol 2:165–169CrossRef Sehgal SN, Gibbons NE (1960) Effect of some metal ions on the growth of halobacterium cutirubrum. Canadian J Microbiol 2:165–169CrossRef
26.
go back to reference Feng EM, Su HY, Wang L (2010) Isolation, identification and phylogenetic analysis of moderately halophilic bacterium isolated from Yantai Sea area. Mar Sci Bul 1:52–58 (Chinese) Feng EM, Su HY, Wang L (2010) Isolation, identification and phylogenetic analysis of moderately halophilic bacterium isolated from Yantai Sea area. Mar Sci Bul 1:52–58 (Chinese)
27.
go back to reference Dong XZ, Cai MY (2001) Common bacterial identification manual. Science Press, Beijing Dong XZ, Cai MY (2001) Common bacterial identification manual. Science Press, Beijing
28.
go back to reference Yoon JH, Kang SJ, Oh KH et al (2009) Salimicrobium flavidum sp. nov., isolated from a marine solar saltern. Int J Syst Evol Microbiol 11:2839–2842CrossRef Yoon JH, Kang SJ, Oh KH et al (2009) Salimicrobium flavidum sp. nov., isolated from a marine solar saltern. Int J Syst Evol Microbiol 11:2839–2842CrossRef
Metadata
Title
Isolation and Characterization of a New Moderately Halophilic Bacterium Strain SM. 200-5 from Solar Saltern Ponds
Authors
Gaochao Xu
Yuangao Deng
Donghui Song
Liying Sui
Copyright Year
2014
Publisher
Springer Berlin Heidelberg
DOI
https://doi.org/10.1007/978-3-642-37922-2_109

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