Recent advances in nanotechnology have provided new experimental tools to study biological processes at the molecular level . Instead of monitoring biochemical reactions involving macroscopic numbers of molecules one can now observe behavior of individual molecules by techniques of single molecule optical and force spectroscopies. The optical spectroscopy has been used primarily for identification of stages in protein folding [2, 3, 4, 5]. On the other hand, the force spectroscopy has been usually applied to establish a degree of mechanical stability through stretching either at constant speed or at constant force to induce unfolding. However, monitoring of the subsequent refolding events in a mechanically controlled environment has also been accomplished [9, 10].
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