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01-06-2022 | Research paper

Micelles of Licorice chalcone A for oral administration: preparation, in vitro, in vivo, and hepatoprotective activity evaluation

Authors: Yuhang Yang, Zhongan Zhu, Michael Adu-Frimpong, Jing Liu, Yaping Wang, Lin Chen, Elmurat Toreniyazov, Hao Ji, Xia Cao, Feng Shi, Qilong Wang, Jiangnan Yu, Ximing Xu

Published in: Journal of Nanoparticle Research | Issue 6/2022

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Abstract

Licorice chalcone A (LCA) possesses a variety of pharmacological activities amid poor solubility in water which consequently restricts its clinical application. In this study, LCA micelles (LCA-M) and LCA micelles modified with TPGS (LCA-M-T) were prepared, wherein in vitro, in vivo, and hepatoprotection potential of the formulations were evaluated. LCA-M-T and LCA-M were prepared by thin-film dispersion method and characterized by a transmission electron microscope (TEM). The encapsulation efficiency, in vitro release, and stability of LCA-M-T and LCA-M were detected by HPLC and UV, respectively. The pharmacokinetics, tissue distribution studies, and pharmacodynamics research were also carried out after oral administration. The sizes of micellar particles were 34.02 ± 0.29 nm (LCA-M) and 62.46 ± 0.51 nm (LCA-M-T) with 0.223 ± 0.021 (LCA-M) and 0.343 ± 0.006 (LCA-M-T) polydispersities, − 35.06 ± 2.27 mV (LCA-M) and − 38.58 ± 1.69 mV (LCA-M-T) zeta potentials, and 95.2 ± 0.27% (LCA-M) and 90.34 ± 0.31% (LCA-M-T) encapsulation efficiencies. The drug loadings were 8.56 ± 0.27% (LCA-M) and 8.04 ± 0.19% (LCA-M-T). The two preparations released quickly and reached over 95% in the four-dissolution media (pH = 1.2 pH = 6.8 pH = 7 pH = 7.4). The preparations of LCA showed significant elevation of cumulative release compared with the free LCA and more importantly 119% (LCA-M) and 285% (LCA-M-T) increments in the relative oral bioavailability of the drug. Preparations of LCA may act as a promising approach to improve solubility and enhance bioavailability and liver protective activity of LCA.

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Title: Micelles of Licorice chalcone A for oral administration: preparation, in vitro, in vivo, and hepatoprotective activity evaluation
Authors: Yuhang Yang
Zhongan Zhu
Michael Adu-Frimpong
Jing Liu
Yaping Wang
Lin Chen
Elmurat Toreniyazov
Hao Ji
Xia Cao
Feng Shi
Qilong Wang
Jiangnan Yu
Ximing Xu
Publication date: 01-06-2022
Publisher: Springer Netherlands
Published in: Journal of Nanoparticle Research / Issue 6/2022
Print ISSN: 1388-0764
Electronic ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-022-05488-2

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