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Published in: Journal of Computational Neuroscience 3/2009

01-12-2009

Modelling and sensitivity analysis of the reactions involving receptor, G-protein and effector in vertebrate olfactory receptor neurons

Authors: Geir Halnes, Erik Ulfhielm, Emma Eklöf Ljunggren, Jeanette Hellgren Kotaleski, Jean-Pierre Rospars

Published in: Journal of Computational Neuroscience | Issue 3/2009

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Abstract

A biochemical model of the receptor, G-protein and effector (RGE) interactions during transduction in the cilia of vertebrate olfactory receptor neurons (ORNs) was developed and calibrated to experimental recordings of cAMP levels and the receptor current (RC). The model describes the steps from odorant binding to activation of the effector enzyme which catalyzes the conversion of ATP to cAMP, and shows how odorant stimulation is amplified and delayed by the RGE transduction cascade. A time-dependent sensitivity analysis was performed on the model. The model output—the cAMP production rate—is particularly sensitive to a few, dominant parameters. During odorant stimulation it depends mainly on the initial density of G-proteins and the catalytic constant for cAMP production.

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Appendix
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Metadata
Title
Modelling and sensitivity analysis of the reactions involving receptor, G-protein and effector in vertebrate olfactory receptor neurons
Authors
Geir Halnes
Erik Ulfhielm
Emma Eklöf Ljunggren
Jeanette Hellgren Kotaleski
Jean-Pierre Rospars
Publication date
01-12-2009
Publisher
Springer US
Published in
Journal of Computational Neuroscience / Issue 3/2009
Print ISSN: 0929-5313
Electronic ISSN: 1573-6873
DOI
https://doi.org/10.1007/s10827-009-0162-6

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