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Inheritance Analysis for Exserted Stigma Rate in Japonica Rice Read chapter Read first chapter

2015 | OriginalPaper | Chapter

21. Molecular Cloning and Characterization of Glycerol Dehydrogenase from Klebsiella pneumoniae

Authors : Yanhua Liu, Li Zhao, Jianguo Zhang, Yu Zheng

Published in: Advances in Applied Biotechnology

Publisher: Springer Berlin Heidelberg

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Abstract

Glycerol dehydrogenase (GDH) was a key enzyme for 1,3-propanediol fermentation from glycerol. In this report, gene gdh, encoding GDH of Klebsiella pneumoniae KG1 was gained by PCR with genomic DNA as template. The open reading frame (ORF) of gdh consisted of 1,143 bp and encoded 380 amino acids with a deduced molecular mass of 42 kDa. The gene gdh was overexpressed in E. coli BL21(DE3) and produced 886-fold higher activity of GDH than that of KG1. The optimum temperature of recombinant GDH was 37 °C and glycerol was the optimum substrate.

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Literature
1.
Ahrens K, Menzel K, Zeng AP et al (1998) Kinetic, dynamic, and pathway studies of glycerol metabolism by Klebsiella pneumoniae in anaerobic continuous culture: III. Enzymes and fluxes of glycerol dissimilation and 1,3-Propanediol formation. Biotechnol Bioeng 59:544–552CrossRef
2.
Biebl H, Marten S (1995) Fermentation of glycerol to 1,3-propanediol: use of cosubstrates. Appl Microbiol Biotechnol 44:15–19CrossRef
3.
Zhang XM, Li Y, Zhuge B (2006) Technical note optimization of 1,3-propanediol production by novel recombinant Escherichia coli using response surface methodology. J Chem Technol Biotechnol 81:1075–1078CrossRef
4.
Zhu MM, Lawman PD, Cameron DC (2002) Improving 1,3-propanediol production from glycerol in a metabolically engineered Escherichia coli by reducing accumulation of sn-glycerol-3-phosphate. Biotechnol Prog 18:694–699CrossRef
5.
Tobimatsu T, Azuma M, Matsubara H (1996) Cloning, sequencing, and high level expression of the genes encoding adenosylcobalamin-dependent glycerol dehydrase of Klebsiella pneumoniae. J Biol Chem 271:22352–22357CrossRef
6.
Céline R, Patricia S, Isabelle MS et al (2003) Molecular characterization of the 1,3-propanediol (1,3-PD) operon of Clostridium butyricum. PNAS 100:5010–5015CrossRef
7.
Zheng YY, Cao Y, Fang BS (2004) Cloning and sequence analysis of the dhaT gene of the 1,3-propanediol regulon from Klebsiella pneumoniae. Biotechnol Lett 26:251–255CrossRef
8.
Seo MY, Seo JW, Heo SY et al (2009) Elimination of by-product formation during production of 1,3-propanediol in Klebsiella pneumoniae by inactivation of glycerol oxidative pathway. Appl Microbiol Biotechnol 84(3):527–534CrossRef
9.
Johnson EA, Levine RL, Lin ECC (1985) Inactivation of glycerol dehydrogenase of Klebsiella pneumoniae and the role of divalent cations. J Bacteriol 164:479–483
10.
Zheng Y, Zhao L, Zhang JG et al (2008) Production of glycerol from glucose by co-expressing glycerol-3-phosphate dehydrogenase and glycerol-3-phosphatase in Klebsiella pneumoniae. J Biosci Bioeng 105(5):508–512CrossRef
11.
Sun JB, Heuvel JVD, Soucaille P et al (2003) Comparative genomic analysis of dha regulon and related genes for anaerobic glycerol metabolism in bacteria. Biotechnol Prog 19:263–272CrossRef
12.
Zheng P, Wereath K, Sun JB (2006) Overexpression of genes of the dha regulon and its effects on cell growth, glycerol fermentation to 1,3-propanediol and plasmid stability in Klebsiella pneumoniae. Process Biochem 41:2160–2169CrossRef
13.
Oh BR, Hong WK, Heo SY et al (2013) The production of 1,3-propanediol from mixtures of glycerol and glucose by a Klebsiella pneumonia mutant deficient in carbon catabolite repression. Bioresour Technol 130:719–724CrossRef
14.
Petrov K, Stoyanov A (2012) Accelerated production of 1,3-propanediol from glycerol by Klebsiella pneumoniae using the method of forced pH fluctuations. Bioprocess Biosyst Eng 35(3):317–321CrossRef
15.
Oh BR, Seo JW, Heo SY et al (2012) Fermentation strategies for 1,3-propanediol production from glycerol using a genetically engineered Klebsiella pneumoniae strain to eliminate by-product formation. Bioprocess Biosyst Eng 35(1–2):159–165CrossRef
Metadata
Title
Molecular Cloning and Characterization of Glycerol Dehydrogenase from Klebsiella pneumoniae
Authors
Yanhua Liu
Li Zhao
Jianguo Zhang
Yu Zheng
Copyright Year
2015
Publisher
Springer Berlin Heidelberg
Book
Advances in Applied Biotechnology

Print ISBN: 978-3-662-46317-8

Electronic ISBN: 978-3-662-46318-5

Copyright Year: 2015

DOI
https://doi.org/10.1007/978-3-662-46318-5_21

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