Skip to main content
Top
Published in: Arabian Journal for Science and Engineering 6/2021

05-01-2021 | Research Article-Biological Sciences

Morphological and Molecular Assessment of Genetic Diversity of Seven Species of the Genus Artemisia L. (Asteraceae)

Authors: Amal H. Al-Ajmi, Mona S. AL-Wahibi, Abd El-Zaher Mustafa, Dina A. Soliman, Yaser Hassan Dewir

Published in: Arabian Journal for Science and Engineering | Issue 6/2021

Log in

Activate our intelligent search to find suitable subject content or patents.

search-config
loading …

Abstract

Species belonging to the genus Artemisia have high economic value and are used in folk and modern medicine. Analysis and identification of their genetic relationships can help in efficient conservation and further utilization of Artemisia genetic resources. This study was conducted to evaluate the genetic diversity among seven species of the genus Artemisia using their morphological characteristics and inter-simple sequence repeat (ISSR)-based molecular markers. The morphology of leaf and achene was examined by light microscopy and scanning electron microscopy. Morphological and molecular data were analyzed using the multivariate statistical package program. There were obvious variations in the morphology of leaf and achene among the Artemisia species. The ISSR results showed a total of 173 amplified loci, consisting of 132 polymorphic loci and 41 unique loci. Polymorphism percentage ranged from 62.5 to 88.8% for nine primers. Mean polymorphism information content for ISSR markers was 0.88, which suggested the efficacy of the ISSR marker system in determining polymorphisms. Significant correlation (r = 0.56, P = 0.004) was observed between similarity coefficients of morphological parameters and ISSR data based on the Mantel test. A wide range of diversity was observed at both morphological and molecular levels among the species. Genetic analyses using ISSR allowed for the detection of relationships between various Artemisia species than was apparent by the analyses of morphological characteristics of these species. Moreover, a mixed pattern of grouping in cluster analysis was found, indicating the close affinities of species with each other and also that the section Artemisia is polyphyletic.

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

  • über 102.000 Bücher
  • über 537 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Finance + Banking
  • Management + Führung
  • Marketing + Vertrieb
  • Maschinenbau + Werkstoffe
  • Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

  • über 67.000 Bücher
  • über 390 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Maschinenbau + Werkstoffe




 

Jetzt Wissensvorsprung sichern!

Literature
1.
go back to reference Bora, K.S.; Sharma, A.: The genus Artemisia: a comprehensive review. Pharm. Biol. 49, 101–109 (2011)CrossRef Bora, K.S.; Sharma, A.: The genus Artemisia: a comprehensive review. Pharm. Biol. 49, 101–109 (2011)CrossRef
2.
go back to reference Mahmood, T.; Hassan, N.; Nazar, N.; Naveed, I.: Phylogenetic analysis of different Artemisia species based on chloroplast gene RPS11. Arch. Biol. Sci. (Beogr.) 63, 661–665 (2011)CrossRef Mahmood, T.; Hassan, N.; Nazar, N.; Naveed, I.: Phylogenetic analysis of different Artemisia species based on chloroplast gene RPS11. Arch. Biol. Sci. (Beogr.) 63, 661–665 (2011)CrossRef
4.
go back to reference Chaudhary, S.A.: Flora of the Kingdom of Saudi Arabia. Ministry of Agriculture and Water, Riyadh (2000) Chaudhary, S.A.: Flora of the Kingdom of Saudi Arabia. Ministry of Agriculture and Water, Riyadh (2000)
5.
go back to reference Valles, J., McArther, E.D.: Artemisia systematic and phylogeny cytogenetic and molecular insight. In: McArthur E.D., Fairbanks D.J. (eds.), comps. Shrubland Ecosystem Genetics and Biodiversity: Proceedings; (2000 June 13–15); Provo, U.T. Proc. RMRS P-21. Ogden, UT: U.S. Department of Agriculture, Forest Service, Rocky Mountain Research Station, pp. 67–74 (2001) Valles, J., McArther, E.D.: Artemisia systematic and phylogeny cytogenetic and molecular insight. In: McArthur E.D., Fairbanks D.J. (eds.), comps. Shrubland Ecosystem Genetics and Biodiversity: Proceedings; (2000 June 13–15); Provo, U.T. Proc. RMRS P-21. Ogden, UT: U.S. Department of Agriculture, Forest Service, Rocky Mountain Research Station, pp. 67–74 (2001)
6.
go back to reference Abad, M.J.; Bedoya, L.M.; Apaza, L.; Bermejo, P.: The Artemisia L. genus: a review of bioactive essential oils. Molecules 17, 2542–2566 (2012)CrossRef Abad, M.J.; Bedoya, L.M.; Apaza, L.; Bermejo, P.: The Artemisia L. genus: a review of bioactive essential oils. Molecules 17, 2542–2566 (2012)CrossRef
7.
go back to reference Willcox, M.: Artemisia species: from traditional medicines to modern antimalarials—and back again. J. Altern. Complement. Med. 15, 101–109 (2009)CrossRef Willcox, M.: Artemisia species: from traditional medicines to modern antimalarials—and back again. J. Altern. Complement. Med. 15, 101–109 (2009)CrossRef
8.
go back to reference Shah, N.C.: The economic and medicinal Artemisia species in India. Scitech J. 1, 29–38 (2014) Shah, N.C.: The economic and medicinal Artemisia species in India. Scitech J. 1, 29–38 (2014)
9.
go back to reference Turuspekov, Y.; Genievskaya, Y.; Baibulatova, A.; Zatybekov, A.; Kotuhov, Y.; Ishmuratova, M.; Imanbayeva, A.; Abugalieva, S.: Phylogenetic taxonomy of Artemisia L. species from Kazakhstan based on MATK analyses. Proc. Latv. Acad. Sci. B 72, 29–37 (2018) Turuspekov, Y.; Genievskaya, Y.; Baibulatova, A.; Zatybekov, A.; Kotuhov, Y.; Ishmuratova, M.; Imanbayeva, A.; Abugalieva, S.: Phylogenetic taxonomy of Artemisia L. species from Kazakhstan based on MATK analyses. Proc. Latv. Acad. Sci. B 72, 29–37 (2018)
10.
go back to reference Hayat, M.Q.: Multiple approaches for taxonomic study of selected Artemisia L. from Pakistan. Quaid-i-Azam. Islamabad, Pakistan (2011) Hayat, M.Q.: Multiple approaches for taxonomic study of selected Artemisia L. from Pakistan. Quaid-i-Azam. Islamabad, Pakistan (2011)
11.
go back to reference Lesica, P.: A Flora of Glacier National Park, Montana. Oregon State University Press, Corvallis (2002) Lesica, P.: A Flora of Glacier National Park, Montana. Oregon State University Press, Corvallis (2002)
12.
go back to reference Pellicer, J., Granatje, T., Valles, J.A.: (Asteraceae): understanding its evolution using cytogenetic and molecular systematic tools, with emphasis on subgenus Dracunculus. Recent Adv. Pharm. Sci. 37, 199–222 (2011) Pellicer, J., Granatje, T., Valles, J.A.: (Asteraceae): understanding its evolution using cytogenetic and molecular systematic tools, with emphasis on subgenus Dracunculus. Recent Adv. Pharm. Sci. 37, 199–222 (2011)
13.
go back to reference Torrell, M.; Garcia-Jacas, N.; Susanna, A.; Vallès, J.: Phylogeny in Artemisia (Asteraceae, Anthemideae) inferred from nuclear ribosomal DNA (ITS) sequences. Taxon 48, 721–736 (1999)CrossRef Torrell, M.; Garcia-Jacas, N.; Susanna, A.; Vallès, J.: Phylogeny in Artemisia (Asteraceae, Anthemideae) inferred from nuclear ribosomal DNA (ITS) sequences. Taxon 48, 721–736 (1999)CrossRef
14.
go back to reference Watson, L.E.; Bates, P.L.; Evans, T.M.; Unwin, M.M.; Estes, J.R.: Molecular phylogeny of Subtribe Artemisiinae (Asteraceae), including Artemisia and its allied and segregate genera. BMC Evol. Biol. 2, 17 (2002)CrossRef Watson, L.E.; Bates, P.L.; Evans, T.M.; Unwin, M.M.; Estes, J.R.: Molecular phylogeny of Subtribe Artemisiinae (Asteraceae), including Artemisia and its allied and segregate genera. BMC Evol. Biol. 2, 17 (2002)CrossRef
15.
go back to reference Haghihhi, A.R.; Belduz, A.O.; Vahed, M.M.; Coskuncelebi, K.; Terzioglu, S.: Phylogenetic relationships among Artemisia species based on nuclear ITS and chloroplast psb A-trn H DNA markers. Biologia 69, 834–839 (2014)CrossRef Haghihhi, A.R.; Belduz, A.O.; Vahed, M.M.; Coskuncelebi, K.; Terzioglu, S.: Phylogenetic relationships among Artemisia species based on nuclear ITS and chloroplast psb A-trn H DNA markers. Biologia 69, 834–839 (2014)CrossRef
16.
go back to reference Malik, S.; Hayat, M.Q.: Cladistics of western Himalayan Artemisia L. (Anthemideae, Asteraceae) reconstructed on morphological traits. Int. J. Bio. Sci. 14, 251–262 (2019) Malik, S.; Hayat, M.Q.: Cladistics of western Himalayan Artemisia L. (Anthemideae, Asteraceae) reconstructed on morphological traits. Int. J. Bio. Sci. 14, 251–262 (2019)
17.
go back to reference Hayat, M.Q.; Ashraf, M.; Jabeen, S.; Shaheen, N.; Yasmin, G.; Khan, M.A.: Taxonomic implication of foliar epidermal characteristics with species reference to stomatal variation in the Genus Artemisia (Asteraceae). Int. J. Agric. Biol. 12, 221–226 (2010) Hayat, M.Q.; Ashraf, M.; Jabeen, S.; Shaheen, N.; Yasmin, G.; Khan, M.A.: Taxonomic implication of foliar epidermal characteristics with species reference to stomatal variation in the Genus Artemisia (Asteraceae). Int. J. Agric. Biol. 12, 221–226 (2010)
18.
go back to reference Liu, C.J.: Geographical distribution of genetic variation in Stylosanthes scabra revealed by RAPD analysis. Euphytica 98, 21–27 (1997)CrossRef Liu, C.J.: Geographical distribution of genetic variation in Stylosanthes scabra revealed by RAPD analysis. Euphytica 98, 21–27 (1997)CrossRef
19.
go back to reference Reiter, S.R.; Young, M.; Scolnik, P.A.: Genetic linkage of the Arabidopsis genome: methods for mapping with recombinant inbreds and random amplified polymorphic DNAs (RAPDs). In: Koncz, C., Chua, N.H., Schell, J. (eds.) Methods in Arabidopsis Research, pp. 170–191. World Scientific Publishing, Singapore (1993) Reiter, S.R.; Young, M.; Scolnik, P.A.: Genetic linkage of the Arabidopsis genome: methods for mapping with recombinant inbreds and random amplified polymorphic DNAs (RAPDs). In: Koncz, C., Chua, N.H., Schell, J. (eds.) Methods in Arabidopsis Research, pp. 170–191. World Scientific Publishing, Singapore (1993)
20.
go back to reference Ferdinandez, Y.S.N.; Somers, D.J.; Coulman, B.E.: Estimating the genetic relationship of hybrid bromegrass to smooth bromegrass and meadow bromegrass using RAPD markers. Plant Breed. 120, 149–153 (2001)CrossRef Ferdinandez, Y.S.N.; Somers, D.J.; Coulman, B.E.: Estimating the genetic relationship of hybrid bromegrass to smooth bromegrass and meadow bromegrass using RAPD markers. Plant Breed. 120, 149–153 (2001)CrossRef
21.
go back to reference Zietkiewicz, E.; Rafalski, A.; Labuda, D.: Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics 20, 176–183 (1994)CrossRef Zietkiewicz, E.; Rafalski, A.; Labuda, D.: Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics 20, 176–183 (1994)CrossRef
22.
go back to reference Harris, J.: RAPDs in systematics a useful methodology? In: Hollingsworth, P.M., Bateman, R.M., Gornall, R.J. (eds.) Molecular Systematics and Plant Evolution, pp. 221–228. Taylor and Francis, London (1999) Harris, J.: RAPDs in systematics a useful methodology? In: Hollingsworth, P.M., Bateman, R.M., Gornall, R.J. (eds.) Molecular Systematics and Plant Evolution, pp. 221–228. Taylor and Francis, London (1999)
23.
go back to reference Wolfe, A.D.; Liston, A.: Contributions of PCR-based methods to plant systematics and evolutionary biology. In: Soltis, D.E., Soltis, P.S., Doyle, J.J. (eds.) Plant Molecular Systematics II, pp. 43–86. Kluwer, Boston (1998)CrossRef Wolfe, A.D.; Liston, A.: Contributions of PCR-based methods to plant systematics and evolutionary biology. In: Soltis, D.E., Soltis, P.S., Doyle, J.J. (eds.) Plant Molecular Systematics II, pp. 43–86. Kluwer, Boston (1998)CrossRef
24.
go back to reference Esselman, E.J.; Jianqiang, L.; Crawford, D.J.; Windus, J.L.; Wolfe, A.D.: Clonal diversity in the rare Calamagrostis porteri ssp. Insperata (Poaceae): comparative results for allozymes and random amplified polymorphic DNA (RAPD) and intersimple sequence repeat (ISSR) markers. Mol. Ecol. 8, 443–451 (1999)CrossRef Esselman, E.J.; Jianqiang, L.; Crawford, D.J.; Windus, J.L.; Wolfe, A.D.: Clonal diversity in the rare Calamagrostis porteri ssp. Insperata (Poaceae): comparative results for allozymes and random amplified polymorphic DNA (RAPD) and intersimple sequence repeat (ISSR) markers. Mol. Ecol. 8, 443–451 (1999)CrossRef
25.
go back to reference Godwin, I.D.; Aitken, E.A.B.; Smith, L.W.: Application of inter simple sequence repeat (ISSR) markers to plant genetics. Electrophoresis 18, 1524–1528 (1997)CrossRef Godwin, I.D.; Aitken, E.A.B.; Smith, L.W.: Application of inter simple sequence repeat (ISSR) markers to plant genetics. Electrophoresis 18, 1524–1528 (1997)CrossRef
26.
go back to reference Badr, A.; Morsy, W.; Abdelfattah, S.; Shams, S.; Shehab, A.: Genetic diversity in Artemisia monosperma and Artemisia judaica populations in Egypt based on morphological, karyological and molecular variations. J. Med. Plants Res. 6, 66–78 (2012) Badr, A.; Morsy, W.; Abdelfattah, S.; Shams, S.; Shehab, A.: Genetic diversity in Artemisia monosperma and Artemisia judaica populations in Egypt based on morphological, karyological and molecular variations. J. Med. Plants Res. 6, 66–78 (2012)
27.
go back to reference Nan, P.; Peng, S.; Shi, S.; Ren, H.; Yang, J.; Zhong, Y.: Interpopulation congruence in Chinese Primula ovalifolia revealed by chemical and molecular markers using essential oils and ISSRs. Z. Naturforsch., C: J. Biosci. 58, 57–61 (2003)CrossRef Nan, P.; Peng, S.; Shi, S.; Ren, H.; Yang, J.; Zhong, Y.: Interpopulation congruence in Chinese Primula ovalifolia revealed by chemical and molecular markers using essential oils and ISSRs. Z. Naturforsch., C: J. Biosci. 58, 57–61 (2003)CrossRef
28.
go back to reference Tharachand, C.; Immanuel, S.C.; Mythili, M.N.: Molecular markers in characterization of medicinal plants: an overview. Res. Plant Biol. 2, 1–12 (2012) Tharachand, C.; Immanuel, S.C.; Mythili, M.N.: Molecular markers in characterization of medicinal plants: an overview. Res. Plant Biol. 2, 1–12 (2012)
29.
go back to reference Bremer, K.; Humphries, C.J.: Generic monograph of the Asteraceae-Anthemideae. Bull. Br. Mus. Nat. Hist. Bot. 23, 71–177 (1993) Bremer, K.; Humphries, C.J.: Generic monograph of the Asteraceae-Anthemideae. Bull. Br. Mus. Nat. Hist. Bot. 23, 71–177 (1993)
30.
go back to reference Ghafoor, A.: Asteraceae (I)—anthemideae. In: Ali, S.I., Qaiser, M. (eds.) Flora of Pakistan 207. Karachi, University of Karachi and St. Louis. Missouri Botanical Garden (2002) Ghafoor, A.: Asteraceae (I)—anthemideae. In: Ali, S.I., Qaiser, M. (eds.) Flora of Pakistan 207. Karachi, University of Karachi and St. Louis. Missouri Botanical Garden (2002)
31.
go back to reference Ling, Y.R.: On the system of genus Artemisia L. and the relationship with its allies. Bull. Bot. Lab. North East. For. Inst. 2, 1–60 (1982) Ling, Y.R.: On the system of genus Artemisia L. and the relationship with its allies. Bull. Bot. Lab. North East. For. Inst. 2, 1–60 (1982)
32.
go back to reference Konowalik, K.; Kreitschitz, A.: Morphological and anatomical characteristics of Artemisia absinthium var. absinthium and its polish endemic variety A. absinthium var. calcigena. Plant Syst. Evol. 298, 1325–1336 (2012)CrossRef Konowalik, K.; Kreitschitz, A.: Morphological and anatomical characteristics of Artemisia absinthium var. absinthium and its polish endemic variety A. absinthium var. calcigena. Plant Syst. Evol. 298, 1325–1336 (2012)CrossRef
33.
go back to reference Malik, S.; Vitales, D.; Hayat, M.Q.; Korobkov, A.A.; Garnatje, T.; Vallès, J.: Phylogeny and biogeography of Artemisia subgenus Seriphidium (Asteraceae, Anthemideae). Taxon 66, 934–952 (2017)CrossRef Malik, S.; Vitales, D.; Hayat, M.Q.; Korobkov, A.A.; Garnatje, T.; Vallès, J.: Phylogeny and biogeography of Artemisia subgenus Seriphidium (Asteraceae, Anthemideae). Taxon 66, 934–952 (2017)CrossRef
34.
go back to reference Nazar, N.; Mahmood, T.: Morphological and molecular characterization of selected Artemisia species from Rawalakot, Azad Jammu and Kashmir. Acta Physiol. Plant. 33, 625–633 (2011)CrossRef Nazar, N.; Mahmood, T.: Morphological and molecular characterization of selected Artemisia species from Rawalakot, Azad Jammu and Kashmir. Acta Physiol. Plant. 33, 625–633 (2011)CrossRef
35.
go back to reference Mohsen, H.; Ali, F.: Study of genetic polymorphism of Artemisia herba-alba from Tunisia using ISSR markers. Afr. J. Biotechnol. 7, 044–050 (2008) Mohsen, H.; Ali, F.: Study of genetic polymorphism of Artemisia herba-alba from Tunisia using ISSR markers. Afr. J. Biotechnol. 7, 044–050 (2008)
36.
go back to reference Elmeer, K.; Elkhgkheg, A.: Genetic diversity of Artemisia herba-alba in Libyan green Mountain. Iran J. Sci Technol. Trans A Sci. 43, 1507–1512 (2019)CrossRef Elmeer, K.; Elkhgkheg, A.: Genetic diversity of Artemisia herba-alba in Libyan green Mountain. Iran J. Sci Technol. Trans A Sci. 43, 1507–1512 (2019)CrossRef
37.
go back to reference Shafie, M.S.B.; Hasan, S.M.; Zain, A.M.; Shah, R.M.: RAPD and ISSR markers for comparative analysis of genetic diversity in wormwood capillary (Artemisia capillaris) from Negeri Sembilan, Malaysia. J. Med. Plants Res. 5, 4426–4437 (2011) Shafie, M.S.B.; Hasan, S.M.; Zain, A.M.; Shah, R.M.: RAPD and ISSR markers for comparative analysis of genetic diversity in wormwood capillary (Artemisia capillaris) from Negeri Sembilan, Malaysia. J. Med. Plants Res. 5, 4426–4437 (2011)
38.
go back to reference Rashmi, T.R.; Murali, S.; Francis, M.S.: ITS2 is an ideal barcode to infer the phylogenetic relationships in Artemisia spp. Int. J. Res. Biosci. 5, 25–32 (2016) Rashmi, T.R.; Murali, S.; Francis, M.S.: ITS2 is an ideal barcode to infer the phylogenetic relationships in Artemisia spp. Int. J. Res. Biosci. 5, 25–32 (2016)
39.
go back to reference Hussain, A.; Potter, D.; Kim, S.; Hayat, M.Q.; Bokhari, S.A.I.: Molecular phylogeny of Artemisia (Asteraceae-Anthemideae) with emphasis on undescribed taxa from Gilgit-Baltistan (Pakistan) based on nrDNA (ITS and ETS) and cpDNA (psbA-trnH) sequences. Plant Ecol. Evol. 152, 507–520 (2019)CrossRef Hussain, A.; Potter, D.; Kim, S.; Hayat, M.Q.; Bokhari, S.A.I.: Molecular phylogeny of Artemisia (Asteraceae-Anthemideae) with emphasis on undescribed taxa from Gilgit-Baltistan (Pakistan) based on nrDNA (ITS and ETS) and cpDNA (psbA-trnH) sequences. Plant Ecol. Evol. 152, 507–520 (2019)CrossRef
40.
go back to reference Shahzadi, I.; Abdullah, M.F.; Ali, Z.; Ahmed, I.; Mirza, B.: Chloroplast genome sequences of Artemisia maritima and Artemisia absinthium: comparative analyses, mutational hotspots in genus Artemisia and phylogeny in family Asteraceae. Genomics 112, 1454–1463 (2020)CrossRef Shahzadi, I.; Abdullah, M.F.; Ali, Z.; Ahmed, I.; Mirza, B.: Chloroplast genome sequences of Artemisia maritima and Artemisia absinthium: comparative analyses, mutational hotspots in genus Artemisia and phylogeny in family Asteraceae. Genomics 112, 1454–1463 (2020)CrossRef
41.
go back to reference Iram, S.; Hayat, M.Q.; Tahir, M.; Gul, A.; Ahmed, I.: Chloroplast genome sequence of Artemisia scoparia: comparative analyses and screening of mutational hotspots. Plants 8, 8 (2019)CrossRef Iram, S.; Hayat, M.Q.; Tahir, M.; Gul, A.; Ahmed, I.: Chloroplast genome sequence of Artemisia scoparia: comparative analyses and screening of mutational hotspots. Plants 8, 8 (2019)CrossRef
42.
go back to reference Li, J.; Jiang, M.; Chen, H.; Yu, J.; Liu, C.: Intraspecific variations among the chloroplast genomes of Artemisia scoparia (Asteraceae) from Pakistan and China. Mitochondrial DNA B 5, 3200–3202 (2020) Li, J.; Jiang, M.; Chen, H.; Yu, J.; Liu, C.: Intraspecific variations among the chloroplast genomes of Artemisia scoparia (Asteraceae) from Pakistan and China. Mitochondrial DNA B 5, 3200–3202 (2020)
43.
go back to reference Kim, G.B.; Lim, C.E.; Kim, J.S.; Kim, K.; Lee, J.H.; Yu, H.J.; Mun, J.H.: Comparative chloroplast genome analysis of Artemisia (Asteraceae) in East Asia: insights into evolutionary divergence and phylogenomic implications. BMC Genom. 21, 415 (2020)CrossRef Kim, G.B.; Lim, C.E.; Kim, J.S.; Kim, K.; Lee, J.H.; Yu, H.J.; Mun, J.H.: Comparative chloroplast genome analysis of Artemisia (Asteraceae) in East Asia: insights into evolutionary divergence and phylogenomic implications. BMC Genom. 21, 415 (2020)CrossRef
44.
go back to reference Zahra, S.; Iraj, M.; Mostafa, A.; Taher, N.: Artemisia herba–alba complex: morphological diversity and molecular uniformity. JBES 5, 608–624 (2014) Zahra, S.; Iraj, M.; Mostafa, A.; Taher, N.: Artemisia herbaalba complex: morphological diversity and molecular uniformity. JBES 5, 608–624 (2014)
45.
go back to reference Doyle, J.J.; Doyle, J.L.: Isolation of plant DNA from fresh tissue. Focus 12, 39–40 (1990) Doyle, J.J.; Doyle, J.L.: Isolation of plant DNA from fresh tissue. Focus 12, 39–40 (1990)
46.
go back to reference Kumar, J.; Mishra, G.P.; Singh, H.; Srivastava, R.B.; Naik, P.K.: Congruence of inter simple sequence repeat (ISSR) and random amplification of polymorphic deoxyribonucleic acid (RAPD) markers in genetic characterization of Artemisia annua in the trans-Himalayan region. J. Med. Plants Res. 5, 5568–5576 (2011) Kumar, J.; Mishra, G.P.; Singh, H.; Srivastava, R.B.; Naik, P.K.: Congruence of inter simple sequence repeat (ISSR) and random amplification of polymorphic deoxyribonucleic acid (RAPD) markers in genetic characterization of Artemisia annua in the trans-Himalayan region. J. Med. Plants Res. 5, 5568–5576 (2011)
47.
go back to reference Mantel, N.: The detection of disease clustering and a generalized regression approach. Cancer Res. 27, 209–220 (1967) Mantel, N.: The detection of disease clustering and a generalized regression approach. Cancer Res. 27, 209–220 (1967)
48.
go back to reference Abid, R.; Qaiser, M.: 2008 Cypsela morphology and its taxonomic significance of the genus Artemisia L. (Anthemideae—Asteraceae) from Pakistan. Pak. J. Bot. 40, 1827–1837 (2008) Abid, R.; Qaiser, M.: 2008 Cypsela morphology and its taxonomic significance of the genus Artemisia L. (Anthemideae—Asteraceae) from Pakistan. Pak. J. Bot. 40, 1827–1837 (2008)
49.
go back to reference Hayat, M.Q.; Ashraf, M.; Khan, M.A.; Mahmood, T.; Ahmed, M.; Jabeen, S.: Phylogeny of Artemisia L: recent developments. Afr. J. Biotechnol. 8, 2423–2428 (2009) Hayat, M.Q.; Ashraf, M.; Khan, M.A.; Mahmood, T.; Ahmed, M.; Jabeen, S.: Phylogeny of Artemisia L: recent developments. Afr. J. Biotechnol. 8, 2423–2428 (2009)
50.
go back to reference Bolaric, S.; Barth, S.; Melchinger, A.E.; Posselt, U.K.: Genetic diversity in European perennial ryegrass cultivars investigated with RAPD markers. Plant Breed. 124, 161–166 (2005)CrossRef Bolaric, S.; Barth, S.; Melchinger, A.E.; Posselt, U.K.: Genetic diversity in European perennial ryegrass cultivars investigated with RAPD markers. Plant Breed. 124, 161–166 (2005)CrossRef
51.
go back to reference Chesnokov, Y.V.; Artemyeva, A.: Evaluation of the measure of polymorphism information of genetic diversity. Agric. Biol. 50, 571–578 (2015) Chesnokov, Y.V.; Artemyeva, A.: Evaluation of the measure of polymorphism information of genetic diversity. Agric. Biol. 50, 571–578 (2015)
52.
go back to reference Nguyen, H.T.; Inotai, K.; Radacsi, P.; Tavasiz-Sarosi Ladanyi, M.; Zamborine-Nemeth, E.: Morphological, phytochemical and molecular characterization of intraspecific variability of wormwood (Artemisia absinthium L). J. Appl. Bot. Food Qual. 90, 238–245 (2017) Nguyen, H.T.; Inotai, K.; Radacsi, P.; Tavasiz-Sarosi Ladanyi, M.; Zamborine-Nemeth, E.: Morphological, phytochemical and molecular characterization of intraspecific variability of wormwood (Artemisia absinthium L). J. Appl. Bot. Food Qual. 90, 238–245 (2017)
53.
go back to reference Somlik, M.; Urbanek, K.; Jadczak, D.: Rzepka-plevnes D (2003) Morphological and genetic variability in some Artemisia species. Acta Hortic. 830, 687–693 (2003) Somlik, M.; Urbanek, K.; Jadczak, D.: Rzepka-plevnes D (2003) Morphological and genetic variability in some Artemisia species. Acta Hortic. 830, 687–693 (2003)
54.
go back to reference Shafie, M.S.B.; Hasan, S.M.Z.; Shah, R.M.: Study of genetic variability of wormwood capillary (Artemisia capillaris) using inter simple sequence repeat (ISSR) in Pahang region, Malaysia. Plant Omics 2, 127–134 (2009) Shafie, M.S.B.; Hasan, S.M.Z.; Shah, R.M.: Study of genetic variability of wormwood capillary (Artemisia capillaris) using inter simple sequence repeat (ISSR) in Pahang region, Malaysia. Plant Omics 2, 127–134 (2009)
Metadata
Title
Morphological and Molecular Assessment of Genetic Diversity of Seven Species of the Genus Artemisia L. (Asteraceae)
Authors
Amal H. Al-Ajmi
Mona S. AL-Wahibi
Abd El-Zaher Mustafa
Dina A. Soliman
Yaser Hassan Dewir
Publication date
05-01-2021
Publisher
Springer Berlin Heidelberg
Published in
Arabian Journal for Science and Engineering / Issue 6/2021
Print ISSN: 2193-567X
Electronic ISSN: 2191-4281
DOI
https://doi.org/10.1007/s13369-020-05189-9

Other articles of this Issue 6/2021

Arabian Journal for Science and Engineering 6/2021 Go to the issue

Premium Partners