Skip to main content
Disciplines
Automotive Business IT + Informatics Construction + Real Estate Electrical Engineering + Electronics Energy + Sustainability Insurance + Risk Finance + Banking Management + Leadership Marketing + Sales Mechanical Engineering + Materials
Events
DE
EN
Books
Journals
Topic Page
Marketing
Start single access now
Access for companies
EXTENDED SEARCH
Log in
Top
Journal of Sol-Gel Science and Technology
Published in: Journal of Sol-Gel Science and Technology 2/2018

26-03-2018 | Original Paper: Sol-gel and hybrid materials for biological and health (medical) applications

Multi-element substituted hydroxyapatites: synthesis, structural characteristics and evaluation of their bioactivity, cell viability, and antibacterial activity

Authors: Abinaya Rajendran, Subha Balakrishnan, Ravichandran Kulandaivelu, Sankara Narayanan T. S. Nellaiappan

Published in: Journal of Sol-Gel Science and Technology | Issue 2/2018

Log in

Activate our intelligent search to find suitable subject content or patents.

search-config
loading …

Abstract

Synthesis of unsubstituted and multi-element (magnesium, zinc and cobalt) substituted hydroxyapatites (HAP) with varying stoichiometric compositions and evaluation of their morphological and structural characteristics, degree of crystallinity, bioactivity, cytotoxicity and antibacterial activity are addressed. The morphological features are not altered much following the substitution of Mg2+, Zn2+, and Co2+ in the HAP lattice. Nevertheless, their substitution exerts a strong influence on the structural characteristics HAP. Rietveld refinement analysis of the X-ray diffraction patterns indicates a decrease in crystallinity and mineralogical composition of HAP phase, which is accompanied with an increase of β-tricalcium phosphate (β-TCP) phase along with Co3O4 phase. Broadening of the PO43− peaks and a decrease in intensity of the OH− peak are observed by Fourier-transform infrared spectra. A decrease in intensity, broadening and a slight shift in Raman band (at 961 cm−1 for HAP) towards the lower side suggest the incorporation of Mg, Zn, and Co, disordering of the crystal structure of HAP and formation of β-TCP as additional phase besides HAP. The MgZnCo-HAP’s exhibits a better bioactivity, cell viability and anti-bacterial activity than the unsubstituted HAP. However, a decrease in cell viability and anti-bacterial activity are observed when the stoichiometric ratio of the substituent elements is relatively higher.
previous article Sol–gel preparation of ZrC–ZrO2 composite microspheres using fructose as a carbon source
next article TiO2/ZnO composite nanodots films and their cellular responses

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

  • über 67.000 Bücher
  • über 390 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Maschinenbau + Werkstoffe




 

Jetzt Wissensvorsprung sichern!

inform now

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

  • über 102.000 Bücher
  • über 537 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Finance + Banking
  • Management + Führung
  • Marketing + Vertrieb
  • Maschinenbau + Werkstoffe
  • Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

inform now
Appendix
Available only for authorised users
Literature
1.
Mucalo M (ed) (2015) Hydroxyapatite (HAp) for biomedical applications, woodhead publishing series in biomaterials. Elsevier-Woodhead Publishers, Cambridge
2.
Dorozhkin SV (2010) Bioceramics of calcium orthophosphates Biomaterials 31:1465–1485CrossRef
3.
Dorozhkin SV (2012) Calcium orthophosphates: applications in Nature, Biology, and Medicine. Pan Stanford Publishing, Singapore
4.
Koutsopoulos S (2002) Synthesis and characterization of hydroxyapatite crystals: A review study on the analytical methods. J Biomed Mater Res 62:600–612CrossRef
5.
Gómez-Morales J, Iafisco M, Delgado-López JM, Sarda S, Drouet C (2013) Progress on the preparation of nanocrystalline apatites and surface characterization: overview of fundamental and applied aspects. Prog Cryst Growth Charact Mater 59:1–46CrossRef
6.
Feng W, Mu-sen L, Yu-peng L, Yong-xin Q (2005) A simple sol–gel technique for preparing hydroxyapatite nanopowders. Mater Lett 59:916–919CrossRef
7.
Fathi MH, Hanifi A (2007) Evaluation and characterization of nanostructure hydroxyapatite powder prepared by simple sol–gel method. Mater Lett 61:3978–3983CrossRef
8.
Shepherd JH, Shepherd DV, Best SM (2012) Substituted hydroxyapatites for bone repair. J Mater Sci Mater Med 23:2335–2347CrossRef
9.
Boanini E, Gazzano M, Bigi A (2010) Ionic substitutions in calcium phosphates synthesized at low temperature. Acta Biomater 6:1882–1894CrossRef
10.
Cacciotti I (2015) Cationic and anionic substitutions in hydroxyapatite. In: Antoniac IV (ed) Handbook of bioceramics and biocomposites. Springer International Publishing, Switzerland, p 1–68
11.
Šupová M (2015) Substituted hydroxyapatites for biomedical applications: A review. Ceram Int 41:9203–9231CrossRef
12.
Percival M (1999) Bone health and osteoporosis. Appl Nutr Sci Rep 5:1–6
13.
Moonga BS, Dempster DW (1995) Zinc is a potent inhibitor of osteoclastic bone resorption in vitro. J Bone Miner Res 10:453–457CrossRef
14.
Yamaguchi M (1998) Role of zinc in bone formation and bone resorption. J Trace Elem Exp Med 11:119–135CrossRef
15.
Aina V, Lusvardi G, Annaz B, Gibson IR, Imrie FE, Malavasi G, Menabue L, Cerrato G, Martra G (2012) Magnesium and strontium-co-substituted hydroxyapatite: the effects of doped-ions on the structure and chemico-physical properties. J Mater Sci Mater Med 23:2867–2879CrossRef
16.
Cox SC, Jamshidi P, Grover LM, Mallick KK (2014) Preparation and characterisation of nanophase Sr, Mg, and Zn substituted hydroxyapatite by aqueous precipitation. Mater Sci Eng C35:106–114CrossRef
17.
Bigi A, Falini G, Foresti E, Gazzano M, Ripamonti A, Roveri N (1993) Magnesium influence on hydroxyapatite crystallization. J Inorg Biochem 49:69–78CrossRef
18.
Landi E, Logroscino G, Proietti L, Tampieri A, Sandri M, Sprio S (2008) Biomimetic Mg substituted hydroxyapatite: from synthesis to in vivo behaviour. J Mater Sci Mater Med 19:239–247CrossRef
19.
Batra U, Kapoor S, Sharma S (2013) Influence of magnesium ion substitution on structural and thermal behavior of nanodimensional hydroxyapatite. J Mater Eng Perform 22:1798–1806CrossRef
20.
Cacciotti I, Bianco A, Lombardi M, Montanaro L (2009) Mg-substituted hydroxyapatite nanopowders: synthesis, thermal stability and sintering behaviour. J Eur Ceram Soc 29:2969–2978CrossRef
21.
Thian ES, Konishi T, Kawanobe Y, Lim PN, Choong C, Ho B, Aizawa M (2013) Zinc-substituted hydroxyapatite: a biomaterial with enhanced bioactivity and antibacterial properties. J Mater Sci Mater Med 24:437–445CrossRef
22.
Bigi A, Foresti E, Gandolfi M, Gazzano M, Roveri N (1995) Inhibiting effect of zinc on hydroxylapatite crystallization. J Inorg Biochem 58:49–58CrossRef
23.
Guerra-Lopez JR, Echeverria GA, Guida JA, Vina R, Punte G (2015) Synthetic hydroxyapatite doped with Zn(II) studied by X-Ray diffraction, infrared, Raman and thermal analysis. J Phys Chem Solids 81:57–65CrossRef
24.
Tang Y, Chappell HF, Dove MT, Reeder RJ, Lee YJ (2009) Zinc incorporation into hydroxylapatite. Biomaterials 30:2864–2872CrossRef
25.
Stanic′ V, Dimitrijevic′ S, Antic′-Stankovic′ J, Mitric′ M, Jokic′ B, Plec′aš I, Raičevic′ S (2010) Synthesis, characterization and antimicrobial activity of copper and zinc-doped hydroxyapatite nanopowders. Appl Surf Sci 256:6083–6089CrossRef
26.
Miyaji F, Kono Y, Suyama Y (2005) Formation and structure of zinc-substituted calcium hydroxyapatite. Mater Res Bull 40:209–220CrossRef
27.
Ren F, Xin R, Ge X, Leng Y (2009) Characterization and structural analysis of zinc-substituted hydroxyapatites. Acta Biomater 5:3141–3149CrossRef
28.
Tank KP, Chudasama KS, Thaker VS, Joshi MJ (2013) Cobalt-doped nano hydroxyapatite: synthesis, characterization, antimicrobial and hemolytic studies. J Nanopart Res 15:1644CrossRef
29.
Stojanovic Z, Veselinovic L, Markovic S, Ignjatovic N, Uskokovic D (2009) Hydrothermal synthesis of nanosized pure and cobalt-exchanged hydroxyapatite. Mater Manuf Process 24:1096–1103CrossRef
30.
Kramer E, Itzkowitz E, Wei M (2014) Synthesis and characterization of cobalt-substituted hydroxyapatite powders. Ceram Int 40:13471–13480CrossRef
31.
Kulanthaivel S, Roy B, Agarwal T, Giri S, Pramanik K, Pal K, Ray SS, Maiti TK, Banerjee I (2016) Cobalt doped proangiogenic hydroxyapatite for bone tissue engineering application. Mater Sci Eng C58:648–658CrossRef
32.
Ignjatović N, Ajduković Z, Savić V, Najman S, Mihailović D, Vasiljević P, Stojanović Z, Uskoković V, Uskoković D (2013) Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones. J Mater Sci Mater Med 24:343–354CrossRef
33.
Moreira MP, Soares GDA, Dentzer J, Anselme K, Sena LÁ, Kuznetsov A, Santos EA (2016) Synthesis of magnesium and manganese-doped hydroxyapatite structures assisted by the simultaneous incorporation of strontium. Mater Sci Eng C61:736–743CrossRef
34.
Iqbal N, Kadir MRA, Mahmood NH, Salim N, Froemming GRA, Balaji HR, Kamarul T (2014) Characterization, antibacterial and in vitro compatibility of zinc–silver doped hydroxyapatite nanoparticles prepared through microwave synthesis. Ceram Int 40:4507–4513CrossRef
35.
Lowry N, Han Y, Meenan BJ, Boyd AR (2017) Strontium and zinc co-substituted nanophase hydroxyapatite. Ceram Int 43:12070–12078CrossRef
36.
Robinson L, Salma-Ancane K, Stipniece L, Meenan BJ, Boyd AR (2017) The deposition of strontium and zinc co-substituted hydroxyapatite coatings. J Mater Sci Mater Med 29:51CrossRef
37.
Kulanthaivel S, Mishra U, Agarwal T, Giri S, Pal K, Pramanik K, Banerjee I (2015) Improving the osteogenic and angiogenic properties of synthetic hydroxyapatite by dual doping of bivalent cobalt and magnesium ion. Ceram Int 41:11323–11333CrossRef
38.
Kolmas J, Jaklewicz A, Zima A, Buc′ko M, Paszkiewicz Z, Lis J, Lósarczyk S ́ (2011) Incorporation of carbonate and magnesium ions into synthetic hydroxyapatite: the effect on physicochemical properties J Mol Struct 987:40–50CrossRef
39.
Suresh Kumar G, Thamizhavel A, Yokogawa Y, Narayana Kalkura S, Girija EK (2012) Synthesis, characterization and in vitro studies of zinc and carbonate co-substituted nano-hydroxyapatite for biomedical applications. Mater Chem Phys 134:1127–1135CrossRef
40.
Gomes S, Vichery C, Descamps S, Martinez H, Kaur A, Jacobs A, Nedelec JM, Renaudin G (2018) Copper doping of calcium phosphate bioceramics from mechanism to the control of cytotoxicity. Acta Biommaterialia 65:465–474
41.
Kokubo T, Kushitani H, Sakka S, Kitsugi T, Yamamuro T (1990) Solutions able to reproduce in vivo surface-structure changes in bioactive glass ceramic A-W3. J Biomed Mater Res 24:721–734CrossRef
42.
Esakkirajan M, Prabhu NM, Arulvasu C, Beulaja M, Manikandan R, Thiagarajan R, Govindarajan K, Prabhu D, Dinesh D, Babu G, Dhanasekaran G (2014) Anti-proliferative effect of a compound isolated from Cassia auriculate against human colon cancer cell line HCT 15. Spectrochim Acta Part A 120:462–466CrossRef
43.
Elkabouss K, Kacimi M, Ziad M, Ammar S, Bozon-Veduraz F (2004) Cobalt-exchanged hydroxyapatite catalysts: magnetic studies, spectroscopic investigations, performance in 2-butanol and ethane oxidative dehydrogenations. J Catal 226:16–24CrossRef
44.
Jarcho M, Bolen CH, Thomas MB, Bobick J, Kay JF, Doremus RH (1976) Hydroxylapatite synthesis and characterization in dense polycrystalline form. J Mater Sci 11:2027–2035CrossRef
45.
Li MO, Xiao XF, Liu RF, Chen CY, Huang LZ (2008) Structural characterization of zinc-substituted hydroxyapatite prepared by hydrothermal method. J Mater Sci Mater Med 19:797–803CrossRef
46.
Shepherd D, Best SM (2013) Production of zinc substituted hydroxyapatite using various precipitation routes. Biomed Mater 8:025003CrossRef
47.
Lorenzo LMR, Regi MV (2000) Controlled crystallization of calcium phosphate apatites. Chem Mater 12:2460–2465CrossRef
48.
Gomes S, Renaudin G, Jallot E, Nedelec JM (2009) Structural characterization and biological fluid interaction of sol-gel derived Mg substituted biphasic phosphate ceramic. App Mater Interface 1(2):505–513CrossRef
49.
Gomes S, Karur A, Nedelec JM, Renaudin G (2014) X-Ray absorption spectroscopy shining (synchrotron) light onto the insertion of Zn2+ in calcium phosphate ceramics and its influence on their behavior under biological conditions. J Mater Chem 2:536–545CrossRef
50.
Gomes S, Karur A, Greneche JM, Nedelec JM, Renaudin G (2017) Atomic scale modeling of iron-doped biphasic calcium phosphate bioceramics. Acta Biomater 50:78–88CrossRef
51.
Renaudin G, Gomes S, Nedelec JM (2017) First-row transition metal doping in calcium phosphate bioceramics: a detailed crystallographic study Mater 10(1):92CrossRef
52.
Gomes S, Nedelec JM, Jallot E, Sheptyakov D, Renaudin G (2011) Unexpected mechanism of Zn2+ insertion in calcium phosphate bioceramics. Chem Mater 23:3072–3085CrossRef
53.
Antonakos A, Liarokapis E, Leventouri T (2007) Micro-Raman and FTIR studies of synthetic and natural apatites. Biomaterials 28:3043–3054CrossRef
54.
Yang Y, Perez-Amodio S, Barre‘ re-de Groot FYF, Everts V, van Blitterswijk CA, Habibovic P (2010) The effects of inorganic additives to calcium phosphate on in vitro behaviour of osteoblasts and osteoclasts. Biomaterials 31:2976–2989CrossRef
55.
Zilm ME, Chen L, Sharma V, McDannald A, Jain M, Ramprasad R, Wei M (2016) Hydroxyapatite substituted by transition metals: experiment and theory. Phys Chem Chem Phys 18:16457–16465CrossRef
56.
Penel G, Leroy G, Rey C, Bres E (1998) Micro Raman spectral study of the PO4 and CO3 vibrational modes in synthetic and biological apatites. Calcif Tissue Int 63:475–481CrossRef
57.
Awonusi A, Morris M, Tecklenburg M (2007) Carbonate assignment and calibration in the Raman spectrum of apatite. Calcif Tissue Int 81:46–52CrossRef
58.
Pasteris JD, Wopenka B, Freeman JJ, Rogers K, Valsami-Jones E, van der Houwen JAM, Silva MJ (2004) Lack of OH in nanocrystalline apatite as a function of degree of atomic order: implications for bone and biomaterials. Biomaterials 25:229–238CrossRef
59.
Darimont GL, Gilbert B, Cloots R, Non-destructive R (2003) evaluation of crystallinity and chemical composition by Raman spectroscopy in hydroxyapatite-coated implants. Mater Lett 58:71–73CrossRef
60.
Aminzadeh A, Shahabi S, Walsh LJ (1999) Raman spectroscopic studies of CO2 laser-irradiated human dental enamel. Spectrochim Acta Part A 55:1303–1308CrossRef
61.
Kim HM, Himeno T, Kokubo T, Nakamura T (2005) Process and kinetics of bonelike apatite formation on sintered hydroxyapatite in a simulated body fluid. Biomaterials 26:4366–4373CrossRef
62.
LeGeros RZ (1993) Biodegradation and bioresorption of calcium phosphate ceramics. Clin Mater 14:65–88CrossRef
63.
Xue W, Liu X, Zheng X, Ding C (2005) Effect of hydroxyapatite coating crystallinity on dissolution and osseointegration in vivo. J Biomed Mater Res A 74:553–561CrossRef
64.
Ding Q, Zhang X, Huang Y, Yan Y, Pang X (2015) In vitro cytocompatibility and corrosion resistance of zinc-doped hydroxyapatite coatings on a titanium substrate. J Mater Sci 50:189–202CrossRef
65.
Wang X, Ito A, Sogo Y, Li X, Oyane A (2010) Zinc-containing apatite layers on external fixation rods promoting cell activity. Acta Biomater 6:962–968CrossRef
66.
Ito A, Ojima K, Naito H, Ichinose N, Tateishi T (2000) Preparation, solubility, and cytocompatibility of zinc-releasing calcium phosphate ceramics. J Biomed Mater Res 50:178–183CrossRef
67.
Lu J, Wei J, Yan Y, Li H, Jia J, Wei S, Guo H, Xiao T, Liu C (2011) Preparation and preliminary cytocompatibility of magnesium doped apatite cement with degradability for bone regeneration. J Mater Sci Mater Med 22:607–615CrossRef
68.
de Lima IR, Alves GG, Soriano CA, Campaneli AP, Gasparoto TH, Ramos Jr ES, de Sena LA, Rossi AM, Granjeiro JM (2011) Understanding the impact of divalent cation substitution on hydroxyapatite: An in vitro multiparametric study on biocompatibility. J Biomed Mater Res Part A 98A:351–358CrossRef
69.
Tin OM, Gopalakrishna V, Samsuddin AR, Al-Salihi KA, Shamsuria O (2007) Antibacterial property of locally produced hydroxyapatite. Arch Orofac Sci 2:41–44
70.
Sahithi K, Swetha M, Prabaharan M, Moorthi A, Saranya N, Ramasamy K, Srinivasan N, Partridge NC, Selvamurugan N (2010) Synthesis and characterization of nanoscale-hydroxyapatite-copper for antimicrobial activity towards bone tissue engineering applications. J Biomed Nanotechnol 6:333–339CrossRef
71.
Kolmas J, Groszyk E, Kwiatkowska-Rozycka D (2014) Substituted hydroxyapatites with antibacterial properties BioMed, Res Int 123:1–15Article ID 178CrossRef
72.
Chung R, Hsieh M, Huang C, Perng L, Wen H, Chin T (2006) Antimicrobial effect and human gingival biocompatibility of hydroxyapatite sol-gel coatings. J Biomed Mater Res B 76B:169–178CrossRef
73.
Swetha M, Sahithi K, Moorthi A, Saranya N, Saravanan S, Ramasamy K, Srinivasan N, Selvamurugan N (2012) Synthesis, characterization, and antimicrobial activity of nano-hydroxyapatite-zinc for bone tissue engineering applications. J Nanosci Nanotechnol 12:167–172CrossRef
74.
75.
Metadata
Title
Multi-element substituted hydroxyapatites: synthesis, structural characteristics and evaluation of their bioactivity, cell viability, and antibacterial activity
Authors
Abinaya Rajendran
Subha Balakrishnan
Ravichandran Kulandaivelu
Sankara Narayanan T. S. Nellaiappan
Publication date
26-03-2018
Publisher
Springer US
Published in
Journal of Sol-Gel Science and Technology / Issue 2/2018
Print ISSN: 0928-0707
Electronic ISSN: 1573-4846
DOI
https://doi.org/10.1007/s10971-018-4634-x

Other articles of this Issue 2/2018

Journal of Sol-Gel Science and Technology 2/2018 Go to the issue

Original Paper: Sol-gel and hybrid materials for catalytic, photoelectrochemical and sensor applications

Investigation of electrocatalytic activity of nanostructure Ce-doped MnO x sol–gel coating deposited on porous Ti membrane electrode

Original Paper: Nano-structured materials (particles, fibers, colloids, composites, etc.)

Sol–gel preparation of ZrC–ZrO2 composite microspheres using fructose as a carbon source

Original Paper: Nano- and macroporous materials (aerogels, xerogels, cryogels, etc.)

Reinforced silica-carbon nanotube monolithic aerogels synthesised by rapid controlled gelation

Original Paper: Fundamentals of sol-gel and hybrid materials processing

Sol-assisted spray-drying synthesis of porous Li3V2(PO4)3/C microspheres as high-activity cathode materials for lithium-ion batteries

Original Paper: Fundamentals of sol-gel and hybrid materials processing

Production of monodisperse cerium oxide microspheres with diameters near 100 µm by internal-gelation sol–gel methods

Original Paper: Nano- and macroporous materials (aerogels, xerogels, cryogels, etc.)

Luminescent alumina-based aerogels modified with tris(8-hydroxyquinolinato)aluminum

Premium Partners

    Image Credits