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2019 | OriginalPaper | Chapter

5. Nanoparticle Behaviour in Complex Media: Methods for Characterizing Physicochemical Properties, Evaluating Protein Corona Formation, and Implications for Biological Studies

Authors : Wye-Khay Fong, Thomas L. Moore, Sandor Balog, Dimitri Vanhecke, Laura Rodriguez-Lorenzo, Barbara Rothen-Rutishauser, Marco Lattuada, Alke Petri-Fink

Published in: Biological Responses to Nanoscale Particles

Publisher: Springer International Publishing

Abstract

The transformation of nanoparticles (NPs) in physiological milieu is a dynamic phenomenon that is the subject of intense investigation. When introduced into the body, NPs can undergo a variety of changes, such as, protein adsorption, dissolution, agglomeration/aggregation, structural deformities and redox reactions. It is these changes that subsequently determine the uptake, bioavailability, translocation and fate of NPs, which ultimately determine their therapeutic efficiency, diagnostic efficacy or toxicity. This chapter will consider the colloidal interactions at the interface of NPs with the contents of biological milieu, the practical and theoretical considerations required to modify analytical and imaging techniques to detect and, if possible, quantify NPs in this complex environment, and the requirement for a highly interdisciplinary approach to understand the behaviour at the bio-nano interface.

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Title: Nanoparticle Behaviour in Complex Media: Methods for Characterizing Physicochemical Properties, Evaluating Protein Corona Formation, and Implications for Biological Studies
Authors: Wye-Khay Fong
Thomas L. Moore
Sandor Balog
Dimitri Vanhecke
Laura Rodriguez-Lorenzo
Barbara Rothen-Rutishauser
Marco Lattuada
Alke Petri-Fink
Publisher: Springer International Publishing
Book: Biological Responses to Nanoscale Particles
Print ISBN: 978-3-030-12460-1

Electronic ISBN: 978-3-030-12461-8

Copyright Year: 2019
DOI: https://doi.org/10.1007/978-3-030-12461-8_5