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Published in:

2015 | Online First | Chapter

Pancreatic Differentiation from Murine Embryonic Stem Cells

Authors : Daisuke Sakano, Nobuaki Shiraki, Shoen Kume, Ph.D

Published in: Methods in Molecular Biology™

Publisher: Springer New York

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Abstract

Pluripotent stem cells are considered as a cell source for replacement therapies for pancreatic beta cells and other organs.
We identified tetrabenazine (TBZ), vesicular monoamine transporter 2 (VMAT2) inhibitor as a promoter of late-stage differentiation of Pdx1-positive pancreatic progenitor cells into Ngn3-positive endocrine progenitor cells. A cell-permeable cAMP analog, dBu-cAMP promotes beta cell maturation in late stage of differentiation. The induced beta cells can secrete insulin in a glucose-dependent manner.
Our protocol consists of a three -step differentiation process. ES cell recapitulate embryonic developmental processes in vitro. Therefore, the ES cell differentiation system is a useful model for the understanding of molecular mechanism of beta-cell differentiation and are useful for application for future regenerative medicine.
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Metadata
Title
Pancreatic Differentiation from Murine Embryonic Stem Cells
Authors
Daisuke Sakano
Nobuaki Shiraki
Shoen Kume, Ph.D
Copyright Year
2015
Publisher
Springer New York
DOI
https://doi.org/10.1007/7651_2015_217