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Published in: Cellulose 9/2020

07-04-2020 | Original Research

Preparation and characterization of magnetic polyporous biochar for cellulase immobilization by physical adsorption

Authors: Haodao Mo, Jianhui Qiu, Chao Yang, Limin Zang, Eiichi Sakai

Published in: Cellulose | Issue 9/2020

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Abstract

Because of the high specific surface area, polyporous structure and ease of preparation, porous biochar from lignocellulosic biomass is popular for being used as support for enzyme immobilization. In this work, polyporous biochar combined with magnetic particle γ-Fe2O3 was prepared by calcination and then used as support for cellulase adsorption. The effects of calcination temperature and time on the properties of magnetic polyporous biochar were investigated and the optimum preparation condition was obtained. For the cellulase adsorption, the immobilization capacity for the magnetic support reached as high as 266 mg/g with a relative activity of 73.6% compared with that of free cellulase. The behavior of cellulase adsorption showed that an endothermic process occurred more easily at high temperatures, which resulted in a high adsorption amount.

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Metadata
Title
Preparation and characterization of magnetic polyporous biochar for cellulase immobilization by physical adsorption
Authors
Haodao Mo
Jianhui Qiu
Chao Yang
Limin Zang
Eiichi Sakai
Publication date
07-04-2020
Publisher
Springer Netherlands
Published in
Cellulose / Issue 9/2020
Print ISSN: 0969-0239
Electronic ISSN: 1572-882X
DOI
https://doi.org/10.1007/s10570-020-03125-6

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