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2017 | OriginalPaper | Chapter

13. Protein Nitrogen Determination

Author : S. Suzanne Nielsen

Published in: Food Analysis Laboratory Manual

Publisher: Springer International Publishing

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Abstract

The protein content of foods can be determined by numerous methods. The Kjeldahl method and the nitrogen combustion (Dumas) method for protein analysis are based on nitrogen determination. Both methods are official for the purposes of nutrition labeling of foods. While the Kjeldahl method has been used widely for over a hundred years, the availability of automated instrumentation for the Dumas method in many cases is replacing use of the Kjeldahl method. This laboratory exercise includes determination of protein by both the Kjeldahl and Dumas methods.

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Literature
go back to reference Chang SKC, Zhang Y (2017) Protein analysis. Ch. 18. In: Nielsen SS (ed) Food analysis, 5th edn. Springer, New York Chang SKC, Zhang Y (2017) Protein analysis. Ch. 18. In: Nielsen SS (ed) Food analysis, 5th edn. Springer, New York
go back to reference AOAC International (2016) Official methods of analysis, 20th edn, (On-line). Method 960.52 (Micro-Kjeldahl method) and Method 992.23 (Generic combustion method). AOAC International, Rockville, MD AOAC International (2016) Official methods of analysis, 20th edn, (On-line). Method 960.52 (Micro-Kjeldahl method) and Method 992.23 (Generic combustion method). AOAC International, Rockville, MD
Metadata
Title
Protein Nitrogen Determination
Author
S. Suzanne Nielsen
Copyright Year
2017
DOI
https://doi.org/10.1007/978-3-319-44127-6_13