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Published in: Journal of Nanoparticle Research 2/2017

01-02-2017 | Research Paper

Quantitative analysis of gold and carbon nanoparticles in mammalian cells by flow cytometry light scattering

Authors: Gang Zhou, Naicheng Liu, Zhenheng Wang, Tongguo Shi, Jingjing Gan, Zhenzhen Wang, Junfeng Zhang

Published in: Journal of Nanoparticle Research | Issue 2/2017

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Abstract

Nanoparticle-based applications for diagnostics and therapeutics have been extensively studied. These applications require a profound understanding of the fate of nanoparticles (NPs) in cellular environments. However, until now, few analytical methods are available and most of them rely on fluorescent properties or special elements of NPs; therefore, for NPs without observable optical properties or special elements, the existing methods are hardly applicable. In this study, we introduce a flow cytometry light scattering (FCLS)-based approach that quantifies in situ NPs accurately in mammalian cells. Continuous cells of heterogeneous human epithelial colorectal adenocarcinoma (Caco-2 cells), mouse peritoneal macrophages (MPM), and human adenocarcinomic alveolar basal epithelia (A549 cells) were cultured with NPs with certain concentrations and size. The intensity of the flow cytometric side scattered light, which indicates the quantity of NPs in the cells, was analyzed. The result shows an accurate size- and dose-dependent uptake of Au NPs (5, 30, 250 nm) in Caco-2 cells. The size- and dose- dependence of Au NPs (5, 30, 250 nm) and carbon NPs (50, 500 nm) in cells was validated by transmission electron microscope (TEM). This paper demonstrates the great potential of flow cytometry light scattering in the quantitative study of the size and dose effect on in situ metallic or non-metallic NPs in mammalian cells.

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Metadata
Title
Quantitative analysis of gold and carbon nanoparticles in mammalian cells by flow cytometry light scattering
Authors
Gang Zhou
Naicheng Liu
Zhenheng Wang
Tongguo Shi
Jingjing Gan
Zhenzhen Wang
Junfeng Zhang
Publication date
01-02-2017
Publisher
Springer Netherlands
Published in
Journal of Nanoparticle Research / Issue 2/2017
Print ISSN: 1388-0764
Electronic ISSN: 1572-896X
DOI
https://doi.org/10.1007/s11051-017-3787-9

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