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Journal of Nanoparticle Research

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01-01-2015 | Research Paper

Short-term effects of ultrahigh concentration cationic silica nanoparticles on cell internalization, cytotoxicity, and cell integrity with human breast cancer cell line (MCF-7)

Authors: Ji Hyun Seog, Bokyung Kong, Dongheun Kim, Lauren M. Graham, Joon Sig Choi, Sang Bok Lee

Published in: Journal of Nanoparticle Research | Issue 1/2015

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Abstract

High concentrations of cationic colloidal silica nanoparticles (CCS-NPs) have been widely used for the enrichment of plasma membrane proteins. However, the interaction between the CCS-NPs and cells under the required concentration for the isolation of plasma membrane are rarely investigated. We evaluated the internalization and toxicity of the 15 nm CCS-NPs which were exposed at high concentrations with short time in human breast cancer cells (MCF-7) with transmission electron microscopy, energy dispersive X-ray spectroscopy, inductively coupled plasma atomic emission spectroscopy, and colorimetric assays. The NPs were observed throughout the cells, particularly in the cytoplasm and the nucleus, after short incubation periods. Additionally, the NPs significantly influenced the membrane integrity of the MCF-7 cells.

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Title: Short-term effects of ultrahigh concentration cationic silica nanoparticles on cell internalization, cytotoxicity, and cell integrity with human breast cancer cell line (MCF-7)
Authors: Ji Hyun Seog
Bokyung Kong
Dongheun Kim
Lauren M. Graham
Joon Sig Choi
Sang Bok Lee
Publication date: 01-01-2015
Publisher: Springer Netherlands
Published in: Journal of Nanoparticle Research / Issue 1/2015
Print ISSN: 1388-0764
Electronic ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-014-2823-2

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