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This study describes stiffness of arterial wall near aortic bifurcation. Uni-axial tensile tests with specimens cut out from human abdominal aorta, aortic bifurcation and common iliac arteries were carried out. Material anisotropy was verified as well as site-specific stiffness. The stiffness was described by initial secant modulus of elasticity (E
). This parameter was gained from stress/strain data at 12% of deformation. The tissue samples in various regions and directions were concluded as described as follows: region of aorto-iliac bifurcation is significantly stiffer in longitudinal direction than in the circumferential direction in all of three examined parts of arterial tree. The comparison between tissue samples cut and loaded in longitudinal direction indicates that arterial tree stiffens in caudal direction. No significant change in material properties was found in the apex of bifurcation in comparison with adjacent tissue. Stiffening of arterial tree during ageing was verified.