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2023 | OriginalPaper | Chapter

Structural and In Vitro Bioactivity of Phosphate-Based Glasses for Bone Regeneration

Authors : M. Mohan Babu, P. Venkateswara Rao, Nibu Putenpurayil Govindan, Raghavendra Gujjala, P. Syam Prasad

Published in: Advances in Glass Research

Publisher: Springer International Publishing

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Abstract

The primary goal of this chapter is to investigate the physical properties, structural makeup, and in vitro bioactivity of a few bioglasses based on phosphate that have been doped with metal ions for bone regeneration and tissue engineering. In this chapter, three series of phosphate glasses have been created using varying amounts of ZnO, TiO2, and Al2O3 and have been characterized using X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and energy dispersive spectrometry (EDS) before and after incubation in the simulated body fluid (SBF) for 3, 7, 14, and 21 days. The physical properties of samples, including density and microhardness, evaluation of pH and weight loss of glasses in physiological fluid and cell culture studies, like cytocompatibility and cell proliferation by seeding rat mesenchymal stem cells (rMSCs) on the glass samples were studied. The results showed that, the density and Vickers microhardness were found to increase with the increase in content of dopants in the phosphate network. The formation of hydroxyapatite (HAp) layer enhancing with immersion time demonstrates the glass sample’s bioactivity. The formation of the apatite layer is supported by the initial rise in pH and the controlled dissolution in SBF. The growth of the rMSCs cells on all samples exhibited good cytocompatibility up to a certain mole percent dependent on the metal ion, as well as a minor decrease with an upsurge in content since the network's rigidity increased. The outcomes support the application compatibility of these glasses involving bone regeneration and repair.

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Literature
2.
5.
go back to reference Bretcanu1, O., Baino, F., Verne, E., & Vitale-Brovarone, C. (2014). Novel resorbable glass-ceramics caffolds for hard tissue engineering: From the parent phosphate glass to its bone-like macroporous derivatives. Journal of Biomaterials Applications, 28, 1287–1303. https://doi.org/10.1177/0885328213506759. Bretcanu1, O., Baino, F., Verne, E., & Vitale-Brovarone, C. (2014). Novel resorbable glass-ceramics caffolds for hard tissue engineering: From the parent phosphate glass to its bone-like macroporous derivatives. Journal of Biomaterials Applications, 28, 1287–1303. https://​doi.​org/​10.​1177/​0885328213506759​.
7.
10.
12.
go back to reference Atkinson, I., Anghel, E. M., Predoana, L., Mocioiu, O. C., Jecu, L., Raut, I., Munteanu, C., Culita, D., & Zaharescu, M. (2016). Influence of ZnO addition on the structural, in vitro behavior and antimicrobial activity of sol–gel derived CaO–P2O5–SiO2 bioactive glasses. Ceramics International, 42, 3033–3045. https://doi.org/10.1016/j.ceramint.2015.10.090.CrossRef Atkinson, I., Anghel, E. M., Predoana, L., Mocioiu, O. C., Jecu, L., Raut, I., Munteanu, C., Culita, D., & Zaharescu, M. (2016). Influence of ZnO addition on the structural, in vitro behavior and antimicrobial activity of sol–gel derived CaO–P2O5–SiO2 bioactive glasses. Ceramics International, 42, 3033–3045. https://​doi.​org/​10.​1016/​j.​ceramint.​2015.​10.​090.CrossRef
19.
go back to reference Babu, M. M., Prasad, P. S., Rao, P. V., Govindan, N. P., Singh, R. K., Kim, H.-W., & Veeraiah, N. (2019). Titanium incorporated Zinc–Phosphate bioactive glasses for bone tissue repair and regeneration: Impact of Ti4+on physico-mechanical and in vitro bioactivity. Ceramics International, 45, 23715–23727. https://doi.org/10.1016/j.ceramint.2019.08.087. Babu, M. M., Prasad, P. S., Rao, P. V., Govindan, N. P., Singh, R. K., Kim, H.-W., & Veeraiah, N. (2019). Titanium incorporated Zinc–Phosphate bioactive glasses for bone tissue repair and regeneration: Impact of Ti4+on physico-mechanical and in vitro bioactivity. Ceramics International, 45, 23715–23727. https://​doi.​org/​10.​1016/​j.​ceramint.​2019.​08.​087.
21.
go back to reference Perozzi, G., Murgia, C., Lang, C., Leong, M., Zalewski, P., Knight, D., Ruffin, R., & Tan, L. W. (2006). Anti-inflammatory effects of zinc and alterations in zinc transporter mRNA in mouse models of allergic inflammation. American Journal of Physiology-Lung Cellular and Molecular Physiology, 292, L577–L584. https://doi.org/10.1152/ajplung.00280.2006. Perozzi, G., Murgia, C., Lang, C., Leong, M., Zalewski, P., Knight, D., Ruffin, R., & Tan, L. W. (2006). Anti-inflammatory effects of zinc and alterations in zinc transporter mRNA in mouse models of allergic inflammation. American Journal of Physiology-Lung Cellular and Molecular Physiology, 292, L577–L584. https://​doi.​org/​10.​1152/​ajplung.​00280.​2006.
23.
go back to reference Boyd, D., Li, H., Tanner, D. A., Towler, M. R., & Wall, J. G. (2006). The antibacterial effects of zinc ion migration from zinc-based glass polyalkenoate cements. Journal of Materials Science: Materials in Medicine, 17, 489–494 (2006). dio: https://doi.org/10.1007/s10856-006-8930-6. Boyd, D., Li, H., Tanner, D. A., Towler, M. R., & Wall, J. G. (2006). The antibacterial effects of zinc ion migration from zinc-based glass polyalkenoate cements. Journal of Materials Science: Materials in Medicine, 17, 489–494 (2006). dio: https://​doi.​org/​10.​1007/​s10856-006-8930-6.
25.
go back to reference Babu, M. M., Prasad, P. S., Bindu, S. H., Prasad, A., Rao, P. V., Govindan, N. P., Veeraiah, N., & Özcan, M. (2020). Investigations on physico-mechanical and spectral studies of Zn2+ doped P2O5-based bioglass system. Journal of Composites Science, 4, 129. https://doi.org/10.3390/jcs4030129.CrossRef Babu, M. M., Prasad, P. S., Bindu, S. H., Prasad, A., Rao, P. V., Govindan, N. P., Veeraiah, N., & Özcan, M. (2020). Investigations on physico-mechanical and spectral studies of Zn2+ doped P2O5-based bioglass system. Journal of Composites Science, 4, 129. https://​doi.​org/​10.​3390/​jcs4030129.CrossRef
33.
37.
go back to reference Abo-naf, S. M., Khalil, E. M., El-sayed, E. M., Zayed, H. A., & Youness, R. A. (2015). In vitro bioactivity evaluation , mechanical properties and microstructural characterization of Na2O–CaO–B2O3–P2O5 glasses. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 144, 88–98. https://doi.org/10.1016/j.saa.2015.02.076. Abo-naf, S. M., Khalil, E. M., El-sayed, E. M., Zayed, H. A., & Youness, R. A. (2015). In vitro bioactivity evaluation , mechanical properties and microstructural characterization of Na2O–CaO–B2O3–P2O5 glasses. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 144, 88–98. https://​doi.​org/​10.​1016/​j.​saa.​2015.​02.​076.
42.
go back to reference Amin Matori, K., Mohd Zaid, M. H., Quah, H. J., Abdul Aziz, S. H., Abdul Wahab, Z., & Mohd Ghazali, M. S. (2015). Studying the effect of ZnO on physical and elastic properties of (ZnO)x (P2O5)1 − x glasses using nondestructive ultrasonic method. Advances in Materials Science and Engineering, 2015, 1–6. https://doi.org/10.1155/2015/596361. Amin Matori, K., Mohd Zaid, M. H., Quah, H. J., Abdul Aziz, S. H., Abdul Wahab, Z., & Mohd Ghazali, M. S. (2015). Studying the effect of ZnO on physical and elastic properties of (ZnO)x (P2O5)1 − x glasses using nondestructive ultrasonic method. Advances in Materials Science and Engineering, 2015, 1–6. https://​doi.​org/​10.​1155/​2015/​596361.
57.
go back to reference Krajewski, A., Mazzocchi, M., Buldini, P. L., Ravaglioli, A., Tinti, A., Taddei, P., & Fagnano, C. (2005). Synthesis of carbonated hydroxyapatites: Efficiency of the substitution and critical evaluation of analytical methods. Journal of Molecular Structure, 744–747, 221–228. https://doi.org/10.1016/j.molstruc.2004.10.044. Krajewski, A., Mazzocchi, M., Buldini, P. L., Ravaglioli, A., Tinti, A., Taddei, P., & Fagnano, C. (2005). Synthesis of carbonated hydroxyapatites: Efficiency of the substitution and critical evaluation of analytical methods. Journal of Molecular Structure, 744–747, 221–228. https://​doi.​org/​10.​1016/​j.​molstruc.​2004.​10.​044.
61.
63.
go back to reference Oki, A., Parveen, B., Hossain, S., Adeniji, S., Donahue, H. (2004). Preparation and in vitro bioactivity of zinc containing sol-gel-derived bioglass materials. Journal of Biomedical Materials Research Part A, 69, 216–221. https://doi.org/10.1002/jbm.a.20070. Oki, A., Parveen, B., Hossain, S., Adeniji, S., Donahue, H. (2004). Preparation and in vitro bioactivity of zinc containing sol-gel-derived bioglass materials. Journal of Biomedical Materials Research Part A, 69, 216–221. https://​doi.​org/​10.​1002/​jbm.​a.​20070.
Metadata
Title
Structural and In Vitro Bioactivity of Phosphate-Based Glasses for Bone Regeneration
Authors
M. Mohan Babu
P. Venkateswara Rao
Nibu Putenpurayil Govindan
Raghavendra Gujjala
P. Syam Prasad
Copyright Year
2023
DOI
https://doi.org/10.1007/978-3-031-20266-7_4

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