Skip to main content
Disciplines
Automotive Business IT + Informatics Construction + Real Estate Electrical Engineering + Electronics Energy + Sustainability Insurance + Risk Finance + Banking Management + Leadership Marketing + Sales Mechanical Engineering + Materials
DE
EN
Books
Journals
Events
Topic Page
Marketing
Log in
Learn more about individual access
Request company access
13th International Engine Congress 2026 | 24 + 25 February 2026

Meeting Place for the Powertrain and Sustainable Fuels Community

Take part as a speaker in the 13th International Engine Congress 2026, the Meeting Place for the Powertrain, and Sustainable Fuels Community for the exchange of experience. Submit a subject proposal online about our main subject areas: Passenger car engine technology, Commercial vehicle engine technology and Sustainable Fuels & Energy!
EXTENDED SEARCH
Log in
Top
Published in:
Read chapter Read first chapter

2023 | OriginalPaper | Chapter

Sustainable Alternative to Antimicrobial Uses: New Probiotics

Authors : Samuel Gómez-Martínez, Apeh Omede, Manuel Gómez-García, Héctor Puente, Lucía Pérez, Ana Carvajal, Héctor Argüello

Published in: Global Challenges for a Sustainable Society

Publisher: Springer International Publishing

Log in

Activate our intelligent search to find suitable subject content or patents.

search-config
loading …

Abstract

Indiscriminate use of antimicrobials is linked to a hazardous increase in antimicrobial resistances (AMR). Global concern in AMR has restricted antimicrobial use through new tight legislation and research on new sustainable alternatives is needed. Probiotics with their ability to positively regulate the growth of beneficial bacteria of the intestinal microbiota are proposed as a solution to this problem. The aim of this study was to isolate and characterise the probiotic potential of 44 lactic acid bacteria (LAB) obtained from sow’s colostrum and milk. Two candidates were selected after determining the AMR profile in this collection for further in vitro assays: a Lactiplantibacillus plantarum (LA-34 M) and a Loigolactobacillus coryniformis (LA-10). Their antibacterial activity was demonstrated against three different enteric pathogens (Escherichia coli, Salmonella spp., and Clostridium perfringens). Adherence test were performed using the epithelial cell line IPI-21, where La-10 and La-34 showed highly adherence to cell culture (64% and 68% respectively). Furthermore, an invasion assay against Salmonella Typhimurium demonstrated that booth strains were able to reduce significantly (p < 0.05) S. Typhimurium invasion after 1 and 2 h of incubation. These results show once more the interest of LAB in pathogen control. Further in vitro and in vivo assays will allow to determine the commercial potential of these two isolates.

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

  • über 102.000 Bücher
  • über 537 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Finance + Banking
  • Management + Führung
  • Marketing + Vertrieb
  • Maschinenbau + Werkstoffe
  • Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

inform now

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

  • über 67.000 Bücher
  • über 390 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Maschinenbau + Werkstoffe




 

Jetzt Wissensvorsprung sichern!

inform now

Springer Professional "Wirtschaft"

Online-Abonnement

Mit Springer Professional "Wirtschaft" erhalten Sie Zugriff auf:

  • über 67.000 Bücher
  • über 340 Zeitschriften

aus folgenden Fachgebieten:

  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Finance + Banking
  • Management + Führung
  • Marketing + Vertrieb
  • Versicherung + Risiko




Jetzt Wissensvorsprung sichern!

inform now
previous chapter Rooftop Solar Photovoltaic Systems for Building of Industrial
next chapter Circular Economy Policies in the Concrete Production
Literature
1.
FAO/OMS: Probiotics in food: health and nutritional properties and guidelines for evaluation. In: FAO Food and Nutrition. World Health Organization, Rome (2006)
2.
European Centre for Disease Prevention and Control. Antimicrobial resistance surveillance in Europe. Surveillance report, pp. 1–131, ECED, Stockholm (2022)
3.
Ma, F., Xu, S., Tang, Z., Li, Z., Zhang, L.: Use of antimicrobials in food animals and impact of transmission of antimicrobial resistance on humans. Biosaf. Heal. 3(1), 32–38 (2021)CrossRef
4.
Regulation (EC) No 1831/2003 of the European Parliament and of the Council of 22 September 2003 on additives for use in animal nutrition (Official Journal of the European Union Brussels: The European Parliament and the Council of the European Union; 18 October, 2003)
5.
Regulation (EU) 2019/6 of the European Parliament and of the Council of 11 December 2018 on veterinary medicinal products and repealing Directive 2001/82/EC (Official Journal of the European Union: The European Parliament and the Council of the European Union; 7 of January, 2019)
6.
7.
Hill, C., Guarner, F., Reid, G., Gibson, G.R., Merenstein, D.J., Pot, B., et al.: Expert consensus document: the international scientific association for probiotics and prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nat. Rev. Gastroenterol. Hepatol. 11(8), 506–514 (2014)
8.
Guarner, F., Malagenada, J.-R.: Gut flora in health and disease. Lancet 36, 512–519 (2003)CrossRef
9.
Rychen, G., Aquilina, G., Azimonti, G., Bampidis, V., Bastos, M.L., et al.: Guidance on the characterisation of microorganisms used as feed additives or as production organisms. EFSA J. 16(3), 1–24 (2018)
10.
Ouwehand, A.C., Salminen, S., Isolauri, E.: Probiotics: an overview of beneficial effects. Int. J. Gen. Mol. Microbiol. 82(1–4), 279–289 (2002)
11.
Tuomola, E., Crittenden, R., Playne, M., Isolauri, E., Salminen, S.: Quality assurance criteria for probiotic bacteria. Am. J. Clin. Nutr. 73(2), 393–398 (2001)CrossRef
12.
Carr, F.J., Chill, D., Maida, N.: The lactic acid bacteria: a literature survey. Crit. Rev. Microbiol. 28, 281–370 (2002)
13.
Jacobsen, C.N., Nielsen, V.R., Hayford, A.E., Møller, P.L., Michaelsen, K.F., Pærregaard, A., et al.: Screening of probiotic activities of forty-seven strains of Lactobacillus spp. by in vitro techniques and evaluation of the colonization ability of five selected strains in humans. Appl. Environ. Microbiol. 65(11), 4949–56 (1999)
14.
Ait Chait, Y., Gunenc, A., Hosseinian, F., Bendali, F.: Antipathogenic and probiotic potential of Lactobacillus brevis strains newly isolated from Algerian artisanal cheeses. Folia Microbiol. (Praha) 66(3), 429–440 (2021)CrossRef
15.
Huys, G., D’Haene, K., Cnockaert, M., Tosi, L., Danielsen, M., Flórez, A.B., et al.: Intra- and interlaboratory performances of two commercial antimicrobial susceptibility testing methods for bifidobacteria and nonenterococcal lactic acid bacteria. Antimicrob. Agents Chemother. 54(6), 2567–2574 (2010)CrossRef
16.
17.
Campedelli, I., Mathur, H., Salvetti, E., Clarke, S., Rea, M.C., Torriani, S., et al.: Genus-wide assessment of antibiotic resistance in Lactobacillus spp. Appl. Environ. Microbiol. 85(1), 1–21 (2019)
18.
Bischoff, K.M., Skinner-Nemec, K.A., Leathers, T.D.: Antimicrobial susceptibility of Lactobacillus species isolated from commercial ethanol plants. J. Ind. Microbiol. Biotechnol. 34(11), 739–44 (2007)
19.
Schillinger, U., Lucke, F.K.: Antimicrobial activity of Lactobacillus sake isolated from meat. Appl. Environ. Microbiol. 55(8), 1901–1906 (1989)CrossRef
20.
Jang, H.J., Kim, J.H., Lee, N.K., Paik, H.D.: Inhibitory effects of Lactobacillus brevis KU15153 against Streptococcus mutans KCTC 5316 causing dental caries. Microb. Pathog. 157, 1–6 (2021)CrossRef
21.
Kaeffer, B., Uriel, I.G., Bottreau, E.: Effects of hypothermia on the survival and cryopreservation of minipig ileal cells and Chinese hamster ovary cells. Cell Biol. Int. 18(11), 1059–1066 (1994)CrossRef
22.
Allaart, J.G., Van Asten, A.J.A.M., Vernooij, J.C.M., Gröne, A.: Beta2 toxin is not involved in in vitro cell cytotoxicity caused by human and porcine cpb2-harbouring Clostridium perfringens. Vet. Microbiol. 171, 132–138 (2014)CrossRef
23.
Fernández, S., Fraga, M., Silveyra, E., Trombert, A.N., Rabaza, A., Pla, M., et al.: Probiotic properties of native Lactobacillus spp. strains for dairy calves. Benef. Microbes 9(4), 613–624 (2014)
24.
Jensen, H., Grimmer, S., Naterstad, K., Axelsson, L.: In vitro testing of commercial and potential probiotic lactic acid bacteria. Int. J. Food Microbiol. 153(2), 216–222 (2012)CrossRef
25.
Das, J.K., Mishra, D., Ray, P., Tripathy, P., Beuria, T.K., Singh, N., et al.: In vitro evaluation of anti- infective activity of a Lactobacillus plantarum strain against Salmonella enterica serovar Enteritidis. Gut. Pathog. 5(1), 1–11 (2013)CrossRef
26.
Jurado, C.A.: Caracterización de la interacción patógeno- hospedador. Aplicación al estudio de la respuesta de células epiteliales intestinales humanas y porcinas frente a la infección por Campylobacter. Universidad de Cordoba (2014)
27.
Zheng, J., Wittouck, S., Salvetti, E., Franz, C.M.A.P., Harris, H.M.B., Mattarelli, P., et al.: A taxonomic note on the genus Lactobacillus: description of 23 novel genera, emended description of the genus Lactobacillus beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae. Int. J. Syst. Evol. Microbiol. 70(4), 2782–858 (2020)
28.
Gopal, P.K., Prasad, J., Smart, J., Gill, H.S.: In vitro adherence properties of Lactobacillus rhamnosus DR20 and Bifidobacterium lactis DR10 strains and their antagonistic activity against an enterotoxigenic Escherichia coli. Int. J. Food Microbiol. 67(3), 207–216 (2001)CrossRef
29.
Rojo-Bezares, B., Sáenz, Y., Poeta, P., Zarazaga, M., Ruiz-Larrea, F., Torres, C.: Assessment of antibiotic susceptibility within lactic acid bacteria strains isolated from wine. Int. J. Food Microbiol. 111(3), 234–240 (2006)CrossRef
30.
Tallon, R., Arias, S., Bressollier, P., Urdaci, M.C.: Strain- and matrix-dependent adhesion of Lactobacillus plantarum is mediated by proteinaceous bacterial compounds. J. Appl. Microbiol. 102(2), 442–451 (2007)CrossRef
31.
Alakomi, H.L., Skyttä, E., Saarela, M., Mattila-Sandholm, T., Latva-Kala, K., Helander, I.M.: Lactic acid permeabilizes gram-negative bacteria by disrupting the outer membrane. Appl. Environ. Microbiol. 66(5), 2001–2005 (2000)CrossRef
32.
Salminen, S.J., Laine, M., Vonwright, A., Vuopio-Varkila, J., Korhonen, T., Mattila-Sandholm, T.: Development of selection criteria for probiotic strains to assess their potential in functional foods: a Nordic and European approach. Biosci. Microflora 2, 61–67 (1996)CrossRef
33.
Garcia-Gonzalez, N., Prete, R., Battista, N., Corsetti, A.: Adhesion properties of food-associated Lactobacillus plantarum strains on human intestinal. Front. Microbiol. 9, 1–11 (2018)CrossRef
34.
Corsetti, A., Ciarrocchi, A., Prete, R.: Lactic acid bacteria: Lactobacillus spp.: Lactobacillus plantarum. Ref. Modul. Food Sci. 1–8 (2016)
35.
Fayol-Messaoudi, D., Berger, C.N., Coconnier-Polter, M.H., Liévin-Le Moal, V., Servin, A.L.: pH-, lactic acid-, and non-lactic acid-dependent activities of probiotic lactobacilli against Salmonella enterica serovar typhimurium. Appl. Environ. Microbiol. 71(10), 6008–6013 (2005)CrossRef
36.
Liu, J., Hu, D., Chen, Y., Huang, H., Zhang, H., Zhao, J., et al.: Strain-specific properties of Lactobacillus plantarum for prevention of Salmonella infection. Food Funct. 9(7), 3673–3682 (2018)
37.
Fontana, L., Bermudez-Brito, M., Plaza-Diaz, J., Muñoz-Quezada, S., Gil, A.: Sources, isolation, characterisation and evaluation of probiotics. Br. J. Nutr. 109, 35–50 (2013)CrossRef
Metadata
Title
Sustainable Alternative to Antimicrobial Uses: New Probiotics
Authors
Samuel Gómez-Martínez
Apeh Omede
Manuel Gómez-García
Héctor Puente
Lucía Pérez
Ana Carvajal
Héctor Argüello
Copyright Year
2023
Book
Global Challenges for a Sustainable Society

Print ISBN: 978-3-031-25839-8

Electronic ISBN: 978-3-031-25840-4

Copyright Year: 2023

DOI
https://doi.org/10.1007/978-3-031-25840-4_49

    Premium Partner

    Image Credits