The enterprising approach of the cyanobacteria and explore its biostimulatory response on Trigonella foenum-graecum (Fabales: Fabaceae)

Cyanobacteria stand out as prospective candidate among various biostimulant microbes due to their ability to produce phytohormone-related substances that stimulate plant growth. In this research line, the bio-stimulatory potential of Nostoc sp. MJU 001 and Scytonemabohneri MBDU 104 on Trigonella foenum-graecum L. seeds was explored. The plant growth-promoting traits of cyanobacteria were evaluated through pot experiments and morphometric growth analysis. Pot experiments were conducted using a 1% biomass extract and the cell suspension of Nostoc sp. MJU 001 and Scytonemabohneri MBDU 104. After 20 days, the growth development profile of Trigonella foenum-graecum L. was assessed through morphometric growth factors. In addition, the quantitative analysis of biochemical characters, including chlorophyll content, total proteins, total soluble phenol, flavonoids, α-amylase activity, and polyphenol activity, was estimated. Moreover, these parameters play a crucial part in the overall health and vitality of the plant. Based on the growth stimulatory potential, the crude extract of the Nostoc sp. MJU 001 was purified by preparative–HPLC and explored through UPLC-MS to identify possible plant growth promoters. The enterprising approach of cyanobacteria biostimulants holds tremendous promise in future agricultural practices. Through their ability to produce phytohormones-related substances, cyanobacteria can stimulate the growth and development of plants, as evidenced by their biostimulatory response to fenugreek. By utilizing cyanobacteria strains like Nostoc sp. MJU 001 and Scytonemabohneri MBDU 104, we can achieve sustainable agriculture while reducing our reliance on synthetic inputs. This research opens avenues for further exploration and implementation of nature-friendly plant nutrition and cultivation solutions.