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Published in:
Effects of Waterlogging and Shading at Jointing and Grain-Filling Stages on Yield Components of Winter Wheat Read chapter Read first chapter

2016 | OriginalPaper | Chapter

The Molecular Detection of Corynespora Cassiicola on Cucumber by PCR Assay Using DNAman Software and NCBI

Author : Weiqing Wang

Published in: Computer and Computing Technologies in Agriculture IX

Publisher: Springer International Publishing

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Abstract

Objective: to establish a quick molecular detection method in Beijing, which can prevent the occurrence of cucumber target spot disease from the source. Methods and results: The DNA band of Corynespora cassiicola had been obtained by PCR and sequenced. Using DNAman software and NCBI database to analyze the sequence, the results showed that the obtained DNA was that of Corynespora cassiicola on Cucumber. A specific primers CCC1/CCC2 were obtained by DNAman software and NCBI database. It was also proved to can be used to distinguish Corynespora cassiicola from other pathogenic fungi using DNAman software and NCBI database.

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previous chapter Comparison of Four Types of Raman Spectroscopy for Noninvasive Determination of Carotenoids in Agricultural Products
next chapter Simulation of Winter Wheat Phenology in Beijing Area with DSSAT-CERES Model
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Metadata
Title
The Molecular Detection of Corynespora Cassiicola on Cucumber by PCR Assay Using DNAman Software and NCBI
Author
Weiqing Wang
Copyright Year
2016
Book
Computer and Computing Technologies in Agriculture IX

Print ISBN: 978-3-319-48353-5

Electronic ISBN: 978-3-319-48354-2

Copyright Year: 2016

DOI
https://doi.org/10.1007/978-3-319-48354-2_26

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