Skip to main content
Top
Published in: Cellulose 11/2021

15-06-2021 | Original Research

Three-phase structure analysis of sawdust cultivation substrates and rapid detection of the optimal water environment for Lentinula edodes growth based on LF-NMR

Authors: Yihong Wang, Shishan Hu, Zhengyan Yang, Qin Jin, Ang Ren, Qin He, Hanshou Yu, Mingwen Zhao

Published in: Cellulose | Issue 11/2021

Log in

Activate our intelligent search to find suitable subject content or patents.

search-config
loading …

Abstract

The three-phase structure of the solid fermentation substrates is composed by the solid, liquid and gas phases, adjusting the ratio of the three-phase structure has proven to be a strategy that can better serve the growth of microorganisms. In this study, the effect of the three-phase structure of the cultivated substrate on the growth of Lentinula edodes was evaluated. Our results showed that the liquid phase was most impacted by the particle size and moisture content among the three-phase. Further analysis of the three-phase structure index (TPSI) showed that the highest efficiency of L. edodes growth was achieved at TPSI value of 23.3, and the liquid phase was the most affected variable on the TPSI of cultivated substrates among the three phases. Further, to determine the optimal water environment for L. edodes in the substrates, three types of water containing four proton components in the cultivation substrate were identified based on distributed exponential curve fitting of transverse relaxation (T2), namely, bound water T2b, free water T21a and T21b, and bulk water T22. L. edodes exhibited the best growth efficiency on elm (59% moisture content), peanut shell (52% moisture content) and apricot (56% moisture content) substrates, all of which were filled with free water and without bulk water, respectively. The same conclusion was also obtained in the mixed material cultivation substrate. In overview, L. edodes had the best growth efficiency in substrates filled with free water and not containing bulk water, which enabled proton analysis based on LF-NMR spectroscopy to provide new insights into the moisture content of wood-rot fungal cultivated substrates.

Graphic abstract

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

  • über 67.000 Bücher
  • über 390 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Maschinenbau + Werkstoffe




 

Jetzt Wissensvorsprung sichern!

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

  • über 102.000 Bücher
  • über 537 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Finance + Banking
  • Management + Führung
  • Marketing + Vertrieb
  • Maschinenbau + Werkstoffe
  • Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

Appendix
Available only for authorised users
Literature
go back to reference Fredriksson M, Thygesen LG (2016) The states of water in Norway spruce (Picea abies (L.). Karst.) studied by low-field nuclear magnetic resonance (LF-NMR) relaxometry: assignment of free-water populations based on quantitative wood anatomy. Holzforschung 71:77–90. https://doi.org/10.1515/hf-2016-0044CrossRef Fredriksson M, Thygesen LG (2016) The states of water in Norway spruce (Picea abies (L.). Karst.) studied by low-field nuclear magnetic resonance (LF-NMR) relaxometry: assignment of free-water populations based on quantitative wood anatomy. Holzforschung 71:77–90. https://​doi.​org/​10.​1515/​hf-2016-0044CrossRef
go back to reference Gang L, Zhinan X, Lin CPA (2000) A morphologically structured model for mycelial growth and secondary metabolite formation. Chin J Chem Eng 8:46–51 Gang L, Zhinan X, Lin CPA (2000) A morphologically structured model for mycelial growth and secondary metabolite formation. Chin J Chem Eng 8:46–51
go back to reference Nitta T, Kamei I, Sugamoto K, Meguro S (2016) Shiitake (Lentinula edodes) cultivation in sawdust media consisting of kunugi (Quercus acutissima) mixed with sugi (Cryptomeria japonica): optimization of gaseous phase rate in media by three-phase-structure analysis. J Wood Sci 6:452–459. https://doi.org/10.1007/s10086-016-1569-0CrossRef Nitta T, Kamei I, Sugamoto K, Meguro S (2016) Shiitake (Lentinula edodes) cultivation in sawdust media consisting of kunugi (Quercus acutissima) mixed with sugi (Cryptomeria japonica): optimization of gaseous phase rate in media by three-phase-structure analysis. J Wood Sci 6:452–459. https://​doi.​org/​10.​1007/​s10086-016-1569-0CrossRef
go back to reference Royse DJ (2009) Cultivation of Shiitake on natural and synthetic logs. The Pennsylvania State University, State College, p 12 Royse DJ (2009) Cultivation of Shiitake on natural and synthetic logs. The Pennsylvania State University, State College, p 12
go back to reference Wei L, Aiwu F, Xiaoming H (2006) Basic parameters of porous media. In: Anqi D (ed) Theory and applications of heat and mass transfer in porous media. Science Press, Beijing Wei L, Aiwu F, Xiaoming H (2006) Basic parameters of porous media. In: Anqi D (ed) Theory and applications of heat and mass transfer in porous media. Science Press, Beijing
Metadata
Title
Three-phase structure analysis of sawdust cultivation substrates and rapid detection of the optimal water environment for Lentinula edodes growth based on LF-NMR
Authors
Yihong Wang
Shishan Hu
Zhengyan Yang
Qin Jin
Ang Ren
Qin He
Hanshou Yu
Mingwen Zhao
Publication date
15-06-2021
Publisher
Springer Netherlands
Published in
Cellulose / Issue 11/2021
Print ISSN: 0969-0239
Electronic ISSN: 1572-882X
DOI
https://doi.org/10.1007/s10570-021-03987-4

Other articles of this Issue 11/2021

Cellulose 11/2021 Go to the issue