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01-04-2012 | Issue 2/2012

Cellulose 2/2012

Tryptophan-based peptides grafted onto oxidized nanocellulose

Journal:
Cellulose > Issue 2/2012
Authors:
Saïd Barazzouk, Claude Daneault

Abstract

Oxidized nanocellulose (ONC) have been synthesized and grafted with tryptophan-based peptides of varying lengths using a two step coupling method. The ONC was first activated by N-ethyl-N′-(3-dimethylaminopropyl) carbodiimide hydrochloride, forming a stable active ester in the presence of N-hydroxysuccinimide. Then, the active ester was reacted with the amino groups of the peptide forming an amide bond between ONC and peptide. Using this method, the intermolecular connection of Trp-based peptides (Trp-Ps) was avoided and uniform coupling of peptides on ONC was achieved. The coupling reaction was very fast in mild conditions and without alteration of the polysaccharide. The obtained products (ONC-Trp-Ps) were characterized by transmission electron microscopy and by different spectroscopic techniques.

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