Top

Biomass Conversion and Biorefinery

Published in:

13-08-2018 | Original Article

Ultrasound-assisted microextraction of β, ε-carotene-3, 3′-diol (lutein) from marine microalgae Chlorella salina: effect of different extraction parameters

Authors: S. Gayathri, S. R. Rajasree Radhika, T. Y. Suman, L. Aranganathan

Published in: Biomass Conversion and Biorefinery | Issue 4/2018

Activate our intelligent search to find suitable subject content or patents.

search-config

AI-assisted search

Off

Abstract

A fast and effective microextraction (ME) technique coupled with ultrasonication (US) was applied for extracting a commercially valuable antioxidant compound lutein, from marine microalgae Chlorella salina (C. salina). The extraction of lutein from C. salina was studied in detail under various operating conditions. Several variables influencing the relative response of the target analyte such as temperature (T = 20–60 °C), time (10–50 min), and frequency (15–55 kHz) were optimized. Results showed maximum yield at temperature 40 °C, extraction time of 30 min duration with 35-kHz frequency. Under optimal conditions, the concentration of lutein was 2.92 ± 0.40 mg/g D.W (dry weight). The results obtained are beneficial for the full utilization of Chlorella biomass, which also indicated that ultrasound-assisted microextraction (US-ME) is a very useful method for extracting lutein from microalgae.

next article Characterization of non-edible lignocellulosic biomass in terms of their candidacy towards alternative renewable fuels

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

über 102.000 Bücher
über 537 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Finance + Banking
Management + Führung
Marketing + Vertrieb
Maschinenbau + Werkstoffe
Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

inform now

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

über 67.000 Bücher
über 390 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Maschinenbau + Werkstoffe

Jetzt Wissensvorsprung sichern!

inform now

Taylor RL, Rand JD, Caldwell GS (2012) Treatment with algae extracts promotes flocculation, and enhances growth and neutral lipid content in Nannochloropsis oculata-a candidate for biofuel production. Mar Biotechnol 14:774–781CrossRef

Fernandez-Sevilla JM, Acien Fernandez FG, Molina Grima E (2010) Biotechnological production of lutein and its applications. Appl Microbiol Biotechnol 86:27–40CrossRef

Del Campo JA, Garcia-Gonzalez M, Guerrero MG (2007) Outdoor cultivation of microalgae for carotenoid production: current state and perspectives. Appl Microbiol Biotechnol 74:1163–1174CrossRef

Lin JH, Lee DJ, Chang JS (2015) Lutein production from biomass: marigold flowers versus microalgae. Bioresour Technol 184:421–428CrossRef

Lakshminarayana R, Aruna G, Sangeetha RK, Bhaskar N, Divakar S, Baskaran V (2008) Possible degradation/biotransformation of lutein in vitro and in vivo: isolation and structural elucidation of lutein metabolites by HPLC and LC-MS (atmospheric pressure chemical ionization). Free Radic Biol Med 45:982–993CrossRef

Seddon JM, Ajani UA, Sperduto RD, Hiller R, Blair N, Burton TC, Farber MD, Gragoudas ES, Haller J, Miller DT et al (1994) Dietary carotenoids, vitamins A, C, and E, and advanced age-related macular degeneration. JAMA 272:1413–1420CrossRef

Olmedilla B, Granado F, Blanco I, Vaquero M (2003) Lutein, but not alpha-tocopherol, supplementation improves visual function in patients with age-related cataracts: a 2-year double blind, placebo-controlled pilot study. Nutrition 19:21–24CrossRef

Krinsky NI, Landrum JT, Bone RA (2003) Biologic mechanisms of the protective role of lutein and zeaxanthin in the eye. Annu Rev Nutr 23:171–193CrossRef

Britton G (1995) Structure and properties of carotenoids in relation to function. FASEB 9:1551–1558CrossRef

10.

Shirsath SR, Sonawane SH, Gogate PR (2012) Intensification of extraction of natural products using ultrasonic irradiations - a review of current status. Chem Eng Process 53:10–23CrossRef

11.

Jadhav D, Rekha BN, Gogate PR, Rathod VK (2009) Extraction of vanillin from vanilla pods: a comparison study of conventional Soxhlet and ultrasound assisted extraction. J Food Eng 93:421–426CrossRef

12.

Luque de Castro MD, Priego-Capote F (2007) Ultrasound assistance to liquid–liquid extraction: a debatable analytical tool. Anal Chim Acta 583:2–9CrossRef

13.

Guo X, Han J, Zhang Y, Wang H, Zhou L (2012) An ammonium sulfate/ethanol aqueous two-phase system combined with ultrasonication for the separation and purification of lithospermic acid B from Salvia miltiorrhiza Bunge. Ultrason Sonochem 19:719–724CrossRef

14.

Li D, Mu C, Cai S, Lin W (2009) Ultrasonic irradiation in the enzymatic extraction of collagen. Ultrason Sonochem 16:605–609CrossRef

15.

Lambropoulou DA, Albanis TA (2007) Liquid-phase micro-extraction techniques in pesticide residue analysis. J Biochem Biophys Methods 70:195–228CrossRef

16.

Jeannot MA, Cantwell FF (1996) Solvent microextraction into a single drop. Anal Chem 68:2236–2240CrossRef

17.

Luque de Castro MD, Priego-Capote F (2007) Ultrasound-assisted preparation of liquid samples. Talanta 72:321–334CrossRef

18.

Luque de Castro MD, Priego-Capote F (2007) Ultrasound assistance to analytical heterogeneous liquid-liquid systems. In: Analytical applications of ultrasound, 1st edn. Elsevier, Amsterdam

19.

Wang J, Sun B, Cao Y, Tian Y, Li X (2008) Optimization of ultrasound-assisted extraction of phenolic compounds from wheat bran. Food Chem 106:804–810CrossRef

20.

Hamdaoui O, Naffrechoux E (2007) An investigation of the mechanisms of ultrasonically enhanced desorption. AICHE J 53:363–373CrossRef

21.

Vilkhu K, Mawson R, Simons L, Bates D (2008) Applications and opportunities for ultrasound assisted extraction in the food industry—a review. Innovative Food Sci Emerg Technol 9:161–169CrossRef

22.

Garcia-Salas P, Morales-Soto A, Segura-Carretero A, Fernandez-Gutierrez A (2010) Phenolic-compound-extraction systems for fruit and vegetable samples. Molecules 15:8813–8826CrossRef

23.

Majd MH, Rajaei A, Bashi DS, Mortazavi SA, Bolourian S (2014) Optimization of ultrasonic-assisted extraction of phenolic compounds from bovine pennyroyal (Phlomidoschema parviflorum) leaves using response surface methodology. Ind Crop Prod 57:195–202CrossRef

24.

Yolmeh M, Najafi MBH, Farhoosh R (2014) Optimization of ultrasound-assisted extraction of natural pigment from annatto seeds by response surface methodology (RSM). Food Chem 155:319–324CrossRef

25.

Rosello-Soto E, Galanakis CM, Brncic M, Orlien V, Trujillo FJ, Mawson R, Knoerzerf K, Tiwari BK, Barba FJ (2015) Clean recovery of antioxidant compounds from plant foods, by-products and algae assisted by ultrasounds processing. Modeling approaches to optimize processing conditions. Trends Food Sci Technol 42:134–149CrossRef

26.

Vuong QV, Thanh DT, Bhuyan DJ, Goldsmith CD, Sadeqzadeh E, Scarlett CJ, Bowyer MC (2015) Optimization of ultrasound-assisted extraction conditions for euphol from the medicinal plant, Euphorbia tirucalli, using response surface methodology. Ind Crop Prod 63:197–202CrossRef

27.

Macias-Sanchez MD, Mantell C, Rodríguez M, Martínez de la Ossa E, Lubian LM, Montero O (2009) Comparison of supercritical fluid and ultrasound-assisted extraction of carotenoids and chlorophyll a from Dunaliella salina. Talanta 77:948–952CrossRef

28.

Lee JY, Yoo C, Jun SY, Ahn CY, Oh HM (2010) Comparison of several methods for effective lipid extraction from microalgae. Bioresour Technol 101:S75–S77CrossRef

29.

Yu X, Dong T, Zheng Y, Miao C, Chen S (2015) Investigations on cell disruption of oleaginous microorganisms: hydrochloric acid digestion is an effective method for lipid extraction. Eur J Lipid Sci Technol 117(5):730–737CrossRef

30.

Gayathri S, Radhika Rajasree SR, Kirubagaran R, Aranganathan L, Suman TY (2016) Spectral characterization of β, ε-carotene-3, 3′-diol (lutein) from marine microalgae Chlorella salina. Renew Energy 98:78–83CrossRef

31.

Deenu A, Naruenartwongsakul S, Kim SM (2013) Optimization and economic evaluation of ultrasound extraction of lutein from Chlorella vulgaris. Biotechnol Bioprocess Eng 18:1151–1162CrossRef

32.

Mandal V, Dewanjee S, Sahu R, Mandal SC (2009) Design and optimization of ultrasound assisted extraction of curcumin as an effective alternative for conventional solid liquid extraction of natural products. Nat Prod Commun 4:95–100

33.

Cenci SM, Cox LR, Leeke GA (2014) Ultrasound-induced emulsification of subcritical carbon dioxide/water with and without surfactant as a strategy for enhanced mass transport. Ultrason Sonochem 21:401–408CrossRef

34.

Daneshvand B, Ara KM, Raofie F (2012) Comparison of supercritical fluid extraction and ultrasound-assisted extraction of fatty acids from quince (Cydonia oblonga Miller) seed using response surface methodology and central composite design. J Chromatogr A 1252:1–7CrossRef

35.

Luo J, Fang Z, Smith RL Jr (2014) Ultrasound-enhanced conversion of biomass to biofuels. Prog Energy Combust Sci 41:56–93CrossRef

36.

Tiwari H, Singh P, Mishra P, Srivastava P (2010) Evaluation of various techniques for extraction of natural colorants from pomegranate rind-ultrasound and enzyme assisted extraction. Indian J Fibre Text Res 35:272–276

37.

Palma M, Taylor LT (1999) Extraction of polyphenolic compounds from grape seeds with near critical carbon dioxide. J Chromatogr A 849:117–124CrossRef

38.

Guo X, Zou X, Sun M (2010) Optimization of extraction process by response surface methodology and preliminary characterization of polysaccharides from Phellinus igniarius. Carbohydr Polym 80:344–349CrossRef

39.

Pingret D, Fabiano-Tixier AS, Bourvellec CL, Renard CMGC, Chemat F (2012) Lab and pilot-scale ultrasound-assisted water extraction of polyphenols from apple pomace. J Food Eng 111(1):73–81CrossRef

40.

Vinatoru M (2001) An overview of the ultrasonically assisted extraction of bioactive principles from herbs. Ultrason Sonochem 8:303–313CrossRef

41.

Luque-Garcia JL, Luque de Castro MD (2004) Ultrasound-assisted Soxhlet extraction: an expeditive approach for solid sample treatment—application to the extraction of Total fat from oleaginous seeds. J Chromatogr A 1034:237–242CrossRef

Title: Ultrasound-assisted microextraction of β, ε-carotene-3, 3′-diol (lutein) from marine microalgae Chlorella salina: effect of different extraction parameters
Authors: S. Gayathri
S. R. Rajasree Radhika
T. Y. Suman
L. Aranganathan
Publication date: 13-08-2018
Publisher: Springer Berlin Heidelberg
Published in: Biomass Conversion and Biorefinery / Issue 4/2018
Print ISSN: 2190-6815
Electronic ISSN: 2190-6823
DOI: https://doi.org/10.1007/s13399-018-0331-9

Springer Professional

Abstract

Please log in to get access to your license.

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Springer Professional "Technik"

Other articles of this Issue 4/2018

Biosilica preparation from abundantly available African biomass Teff (Eragrostis tef) straw ash by sol-gel method and its characterization

Valorization of humins by phosphoric acid activation for activated carbon production

Microwave-assisted conversion of novel biomass materials into levulinic acid

Experimental studies on production of deoxygenated vegetable oils and their performance evaluation in a compression ignition engine

Microstructured devices for biodiesel production by transesterification

Deconstruction of hybrid poplar to monomeric sugars and aromatics using ethanol organosolv fractionation