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Journal of Nanoparticle Research

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01-01-2015 | Research Paper

Uptake of cerium oxide nanoparticles and its influence on functions of mouse leukemic monocyte macrophages

Authors: Xiangyan Zhou, Bing Wang, Pengfei Jiang, Yiqi Chen, Zhengwei Mao, Changyou Gao

Published in: Journal of Nanoparticle Research | Issue 1/2015

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Abstract

Exposure of the CeO₂ nanoparticles (NPs) causes a public concern on their potential health risk due to their wide applications in the fields of fuel additive, commodities, pharmaceutical, and other industries. In this study, the interactions between two commercial CeO₂ NPs (D-CeO₂ from Degussa and PC-CeO₂ from PlasmaChem) and mouse leukemic monocyte macrophage Raw264.7 cells were investigated to provide a fast and in-depth understanding of the biological influences of the NPs. Both types of the CeO₂ NPs had a negative surface charge around −12 mV and showed a tendency to form aggregates with sizes of 191 ± 5.9 and 60.9 ± 2.8 nm in cell culture environment, respectively. The cellular uptake of the CeO₂ NPs increased along with the increase of feeding dosage and prolongation of the culture time. The PC-CeO₂ NPs had a faster uptake rate and reached higher cellular loading amount at the highest feeding concentration (200 µg/mL). In general, both types of the CeO₂ NPs had rather small cytotoxicity even with a dosage as high as 200 µg/mL. The D-CeO₂ NPs showed a relative stronger cytotoxicity especially at higher concentrations and longer incubation time. The NPs were dispersed in vacuoles (most likely endosomes and lysosomes) and cytoplasm. Although both types of the CeO₂ NPs could suppress the production of reactive oxygen species, they impaired the mitochondria membrane potential to some extent. The cytoskeleton organization was altered and consequently the cell adhesion ability decreased after uptake of both types of the CeO₂ NPs.

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Title: Uptake of cerium oxide nanoparticles and its influence on functions of mouse leukemic monocyte macrophages
Authors: Xiangyan Zhou
Bing Wang
Pengfei Jiang
Yiqi Chen
Zhengwei Mao
Changyou Gao
Publication date: 01-01-2015
Publisher: Springer Netherlands
Published in: Journal of Nanoparticle Research / Issue 1/2015
Print ISSN: 1388-0764
Electronic ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-014-2815-2

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