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Published in:

2015 | Online First | Chapter

Using Oct4:MerCreMer Lineage Tracing to Monitor Endogenous Oct4 Expression During the Reprogramming of Fibroblasts into Induced Pluripotent Stem Cells (iPSCs)

Authors : Lucas V. Greder, Jason Post, James R. Dutton

Published in: Methods in Molecular Biology™

Publisher: Springer New York

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Abstract

The reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) using a combination of defined transcription factors has become one of the most widely used techniques in stem cell biology. A critical, early event in iPSC reprogramming is the induction of the endogenous transcription factor network that maintains pluripotency in iPSCs. Here we describe using a transgenic, conditional Oct4-Cre construct to investigate the spatial and temporal induction of endogenous Oct4 expression during the reprogramming of mouse fibroblasts into iPS cells.
Literature
1.
Okita K, Ichisaka T, Yamanaka S (2007) Generation of germline-competent induced pluripotent stem cells. Nature 448(7151):313–317PubMedCrossRef
2.
Takahashi K, Yamanaka S (2006) Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126(4):663–676PubMedCrossRef
3.
Park IH et al (2008) Reprogramming of human somatic cells to pluripotency with defined factors. Nature 451(7175):141–146PubMedCrossRef
4.
Takahashi K et al (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131(5):861–872PubMedCrossRef
5.
Brambrink T et al (2008) Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. Cell Stem Cell 2(2):151–159PubMedPubMedCentralCrossRef
6.
7.
Ovitt CE, Scholer HR (1998) The molecular biology of Oct-4 in the early mouse embryo. Mol Hum Reprod 4(11):1021–1031PubMedCrossRef
8.
Scholer HR et al (1990) Oct-4: a germline-specific transcription factor mapping to the mouse t-complex. EMBO J 9(7):2185–2195PubMedPubMedCentral
9.
Greder LV et al (2012) Analysis of endogenous Oct4 activation during induced pluripotent stem cell reprogramming using an inducible Oct4 lineage label. Stem Cells 30(11):2596–2601PubMedPubMedCentralCrossRef
10.
11.
12.
Morita S, Kojima T, Kitamura T (2000) Plat-E: an efficient and stable system for transient packaging of retroviruses. Gene Ther 7(12):1063–1066PubMedCrossRef
13.
Hirai H et al (2011) Radical acceleration of nuclear reprogramming by chromatin remodeling with the transactivation domain of MyoD. Stem Cells 29(9):1349–1361PubMedPubMedCentral
Metadata
Title
Using Oct4:MerCreMer Lineage Tracing to Monitor Endogenous Oct4 Expression During the Reprogramming of Fibroblasts into Induced Pluripotent Stem Cells (iPSCs)
Authors
Lucas V. Greder
Jason Post
James R. Dutton
Copyright Year
2015
Publisher
Springer New York
DOI
https://doi.org/10.1007/7651_2015_198