Top

Journal of Materials Science

Published in:

10-02-2021 | Materials for life sciences

Utilization of polyvinyl butyral-zirconium alkoxide hybrid hollow tube as an enzyme immobilization carrier

Authors: Anamul Hoque Bhuiyan, Takuma Nagakawa, Mohammad Zakaria, Koji Nakane

Published in: Journal of Materials Science | Issue 14/2021

Activate our intelligent search to find suitable subject content or patents.

search-config

AI-assisted search

Off

Abstract

We developed a Polyvinyl butyral (PVB)-zirconium alkoxide hybrid hollow tube by air-gap spinning. Enzyme (β-galactosidase and lipase) was physically entrap-immobilized and disseminated throughout the hollow tube architecture. The enzyme immobilized PVB-Zr hybrid tube shown its stability in phosphate buffer solution, electrolyte solution, and other organic solvents. The apparent Michaelis constant K_m for immobilized β-galactosidase (IβG) was 0.079 mol/l compared to 0.067 mol/l for free enzyme. The maximum velocity V_max was 4.9 μmol.min⁻¹, whereas it was 11.5 μmol.min⁻¹ for free enzyme. The preserved activity of IβG was found to be 90%, after ten reaction cycles. Moreover, up to 70% conversion for citronellyl acetate after 50 h. was achieved by immobilized lipase (IL) in hexane solution. The activity of IL was found to be 62% after 8 repeated uses where free enzyme retained 15% activity. Overall, it was considered that the enzyme activity employed both in the interior and the vicinity of the hollow tube surface.

Graphical Abstract

previous article Ti-grafted MIL-101 as hydrophilic catalyst for efficient oxidative desulfurization of dibenzothiophene with hydrogen peroxide

next article Continuous high-pressure torsion of pure Al and Al-2 wt% Fe alloy using multi-wires

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

über 102.000 Bücher
über 537 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Finance + Banking
Management + Führung
Marketing + Vertrieb
Maschinenbau + Werkstoffe
Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

inform now

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

über 67.000 Bücher
über 390 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Maschinenbau + Werkstoffe

Jetzt Wissensvorsprung sichern!

inform now

Wen J, Wilkes GL (1996) Organic, Inorganic hybrid network materials by the sol- gel approach. Chem Mater 8:1667–1681CrossRef

Ansari SA, Satar R, Kashif Zaidi S, Ahmad A (2014) Immobilization of Aspergillus oryzae β-galactosidase on cellulose acetate-polymethylmethacrylate membrane and its application in hydrolysis of lactose from milk and whey. Int Sch Res Not. https://doi.org/10.1155/2014/163987CrossRef

Ikeda Y, Kurokawa Y, Nakane K, Ogata N (2002) Entrap-immobilization of biocatalysts on cellulose acetate-inorganic composite gel fiber using a gel formation ofcellulose acetate–metal (Ti, Zr) alkoxide. Cellulose 9:369–379CrossRef

Haghju S, Bari MR, Khaled-Abad MA (2018) Affecting parameters on fabrication of β-D-galactosidase immobilized chitosan/poly (vinyl alcohol) electrospun nanofibers. Carbohyd Polym 200:137–143CrossRef

Vasileva N, Iotov V, Ivanov Y et al (2012) Immobilization of β-galactosidase on modified polypropilene membranes. Int J Biol Macromol 51:710–719. https://doi.org/10.1016/j.ijbiomac.2012.07.032CrossRef

Monteiro RR, Lima PJ, Pinheiro BB et al (2019) Immobilization of lipase A from Candida antarctica onto chitosan-coated magnetic nanoparticles. Int J Mol Sci 20:4018CrossRef

Won K, Kim S, Kim K-J et al (2005) Optimization of lipase entrapment in Ca-alginate gel beads. Process Biochem 40:2149–2154CrossRef

Lang W-Z, Shen J-P, Zhang Y-X et al (2013) Preparation and characterizations of charged poly (vinyl butyral) hollow fiber ultrafiltration membranes with perfluorosulfonic acid as additive. J Membr Sci 430:1–10CrossRef

Nakane K, Mizutani K, Zhang R et al (2012) Poly (vinyl butyral)-zirconia hybrid films formed by sol-gel process. World J Eng 9(3):233–238CrossRef

10.

Nakane K, Ogihara T, Ogata N, Kurokawa Y (2003) Formation of composite gel fiber from cellulose acetate and zirconium tetra-n-butoxide and entrap-immobilization of β-galactosidase on the fiber. J Mater Res 18:672–676. https://doi.org/10.1557/JMR.2003.0089CrossRef

11.

Nakane K, Kuranobu K, Ogihara T et al (2003) Synthesis of citronellyl acetate by lipase entrap-immobilized in cellulose acetate-zirconium butoxide gel fiber. Sen’i Gakkaishi 59:99–103. https://doi.org/10.2115/fiber.59.99CrossRef

12.

White CA, Kennedy JF (1980) Popular matrices for enzyme and other immobilizations. Enzyme Microb Technol 2:82–90CrossRef

13.

Bhuiyan AH, Nagakawa T, Nakane K (2021) Polyvinyl butyral-zirconia hybrid hollow fibers prepared by air gap spinning. J Appl Polym Sci 138:50164. https://doi.org/10.1002/app.50164CrossRef

14.

Betancor L, Luckarift HR, Seo JH et al (2008) Three-dimensional immobilization of β-galactosidase on a silicon surface. Biotechnol Bioeng 99:261–267CrossRef

15.

Wang Y, Wang X, Luo G, Dai Y (2008) Adsorption of bovin serum albumin (BSA) onto the magnetic chitosan nanoparticles prepared by a microemulsion system. Biores Technol 99:3881–3884CrossRef

16.

Synowiecki J, Wo\losowska S (2006) Immobilization of thermostable β-glucosidase from Sulfolobus shibatae by cross-linking with transglutaminase. Enzyme Microb Technol 39:1417–1422CrossRef

17.

Salman S, Soundararajan S, Safina G et al (2008) Hydroxyapatite as a novel reversible in situ adsorption matrix for enzyme thermistor-based FIA. Talanta 77:490–493CrossRef

18.

D’souza SF (1999) Immobilized enzymes in bioprocess. Curr Sci 77:69–79

19.

Ikeda Y, Kurokawa Y (2001) Synthesis of geranyl acetate by lipase entrap-immobilized in cellulose acetate-TiO2 gel fiber. J Amer Oil Chem Soc 78:1099–1103. https://doi.org/10.1007/s11746-001-0396-7CrossRef

20.

Elnashar MM, Awad GE, Hassan ME et al (2014) Optimal immobilization of β-galactosidase onto κ-carrageenan gel beads using response surface methodology and its applications. Sci World J. https://doi.org/10.1155/2014/571682CrossRef

21.

Mateo C, Palomo JM, Fernandez-Lorente G et al (2007) Improvement of enzyme activity, stability and selectivity via immobilization techniques. Enzyme Microb Technol 40:1451–1463CrossRef

22.

Haider T, Husain Q (2009) Immobilization of β galactosidase from Aspergillus oryzae via immunoaffinity support. Biochem Eng J 43:307–314CrossRef

23.

Datta S, Christena LR, Rajaram YRS (2013) Enzyme immobilization: an overview on techniques and support materials. 3 Biotech 3(1):1–9CrossRef

24.

Dos Santos JC, Bonazza HL, de Matos LJ et al (2017) Immobilization of CALB on activated chitosan: application to enzymatic synthesis in supercritical and near-critical carbon dioxide. Biotechnol Rep 14:16–26CrossRef

25.

Barbosa O, Torres R, Ortiz C et al (2013) Heterofunctional supports in enzyme immobilization: from traditional immobilization protocols to opportunities in tuning enzyme properties. Biomacromol 14:2433–2462CrossRef

26.

Hanefeld U, Gardossi L, Magner E (2009) Understanding enzyme immobilisation. Chem Soc Rev 38:453–468CrossRef

27.

Sheldon RA, van Pelt S (2013) Enzyme immobilisation in biocatalysis: why, what and how. Chem Soc Rev 42:6223–6235CrossRef

28.

Garcia-Galan C, Berenguer-Murcia Á, Fernandez-Lafuente R, Rodrigues RC (2011) Potential of different enzyme immobilization strategies to improve enzyme performance. Adv Synth Catal 353:2885–2904CrossRef

29.

Rodrigues RC, Ortiz C, Berenguer-Murcia Á et al (2013) Modifying enzyme activity and selectivity by immobilization. Chem Soc Rev 42:6290–6307CrossRef

30.

Nakane K, Hotta T, Ogihara T, Ogata N, Yamaguchi S (2007) Synthesis of (Z)‐3‐hexen‐1‐yl acetate by lipase immobilized in polyvinyl alcohol nanofibers. J Appl Polym Sci 106:863–867. https://doi.org/10.1002/app.26710

Title: Utilization of polyvinyl butyral-zirconium alkoxide hybrid hollow tube as an enzyme immobilization carrier
Authors: Anamul Hoque Bhuiyan
Takuma Nagakawa
Mohammad Zakaria
Koji Nakane
Publication date: 10-02-2021
Publisher: Springer US
Published in: Journal of Materials Science / Issue 14/2021
Print ISSN: 0022-2461
Electronic ISSN: 1573-4803
DOI: https://doi.org/10.1007/s10853-021-05829-x

Springer Professional

Abstract

Graphical Abstract

Please log in to get access to your license.

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Springer Professional "Technik"

Other articles of this Issue 14/2021

Hand-extended noodle inspired physical conjoined-network organohydrogels with anti-freezing, high stiffness and toughness properties

BiInO3 phases under asymmetric in-plane strain

Dynamic strain aging behavior of accident tolerance fuel cladding FeCrAl-based alloy for advanced nuclear energy

Robust and ultrasensitive hydrogel sensors enhanced by MXene/cellulose nanocrystals

Improvement of poly(propylidene carbonate) mechanical properties by using stereocomplex crystals of polylactide to anchor graphene oxide at the interface of poly(propylidene carbonate)/poly(L-lactide) blends

Insights of enhanced oxygen evolution reaction of nanostructured cobalt ferrite surface

Premium Partners