Top

Published in:

2025 | OriginalPaper | Chapter

Valorisation of Recycled Concrete Aggregates via Calcium Carbonate Precipitation Through Urease Enzyme Extracted from Watermelon Seeds

Authors : Snigdha Bhutange, Salman Muhammad

Published in: Proceedings of the RILEM Spring Convention and Conference 2024

Publisher: Springer Nature Switzerland

Activate our intelligent search to find suitable subject content or patents.

search-config

AI-assisted search

Off

Abstract

The current study attempts to utilize crude urease extracted from watermelon seeds along with urea and calcium source, for the treatment of recycled concrete aggregates. The experimental program involved extraction of urease in a phosphate-EDTA buffer to ensure the stability of the active sites of the enzyme. Its activity was assessed using spectrophotometric method. Subsequently, varying concentrations of this crude urease extract were used to treat recycled concrete aggregates at different temperatures. The results indicated a significant reduction in water absorption for the treated aggregates compared to untreated ones. Furthermore, image analysis of scanning electron images revealed a notable decrease in total porosities of the treated recycled aggregates compared to their untreated counterparts. X-ray diffraction analysis confirmed the presence of calcite, vaterite and aragonite polymorphs of calcium carbonate in the precipitates produced by the activity of the urease enzyme.

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

über 102.000 Bücher
über 537 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Finance + Banking
Management + Führung
Marketing + Vertrieb
Maschinenbau + Werkstoffe
Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

inform now

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

über 67.000 Bücher
über 390 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Maschinenbau + Werkstoffe

Jetzt Wissensvorsprung sichern!

inform now

Springer Professional "Wirtschaft"

Online-Abonnement

Mit Springer Professional "Wirtschaft" erhalten Sie Zugriff auf:

über 67.000 Bücher
über 340 Zeitschriften

aus folgenden Fachgebieten:

Bauwesen + Immobilien
Business IT + Informatik
Finance + Banking
Management + Führung
Marketing + Vertrieb
Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

inform now

previous chapter Exploring Sustainable Alternatives to OPC: Incorporation of Recycled Ceramics into Limestone Calcined Clay Cement

next chapter Use of Limestone Calcinated Clay in Concrete

Mistri, A., Bhattacharyya, S.K., Dhami, N., Mukherjee, A., Barai, S.V.: A review on different treatment methods for enhancing the properties of recycled aggregates for sustainable construction materials. Constr. Build. Mater. 233, 117894 (2020). https://doi.org/10.1016/j.conbuildmat.2019.117894

Tam, V.W., Tam, C.M.: Diversifying two-stage mixing approach (TSMA) for recycled aggregate concrete: TSMAs and TSMAsc. Constr. Build. Mater. 22(10), 2068–2077 (2008). https://doi.org/10.1016/j.conbuildmat.2007.07.024CrossRef

Tam, V.W., Gao, X.F., Tam, C.M.: Microstructural analysis of recycled aggregate concrete produced from two-stage mixing approach. Cem. Concr. Res. 35(6), 1195–1203 (2005). https://doi.org/10.1016/j.cemconres.2004.10.025CrossRef

Qiu, J., Tng, D.Q.S., Yang, E.H.: Surface treatment of recycled concrete aggregates through microbial carbonate precipitation. Constr. Build. Mater. 57, 144–150 (2014). https://doi.org/10.1016/j.conbuildmat.2014.01.085CrossRef

Bhutange, S.P., Latkar, M.V., Chakrabarti, T.: Influence of direct urease source incorporation on mechanical properties of concrete. Constr. Build. Mater. 301, 124116 (2021). https://doi.org/10.1016/j.conbuildmat.2021.124116CrossRef

Junwale, R.D., Bhutange, S.P., Latkar, M.V.: Effect of enzyme Induced carbonate precipitate on mechanical properties of PPC Mortar. Mater. Today Proc. (2023). https://doi.org/10.1016/j.matpr.2023.02.359

Junwale, R., Nikode, A., Bhutange, S., Latkar, M.V.: Crack healing in cement mortar using enzyme induced calcium carbonate precipitation. Constr. Build. Mater. 394, 132223 (2023). https://doi.org/10.1016/j.conbuildmat.2023.132223CrossRef

Khodadadi Tirkolaei, H., Javadi, N., Krishnan, V., Hamdan, N., Kavazanjian Jr., E.: Crude urease extract for biocementation. J. Mater. Civ. Eng. 32(12), 04020374 (2020). https://doi.org/10.1061/(ASCE)MT.1943-5533.0003466

Dilrukshi, R.A.N., Nakashima, K., Kawasaki, S.: Soil improvement using plant-derived urease-induced calcium carbonate precipitation. Soils Found. 58(4), 894–910 (2018). https://doi.org/10.1016/j.sandf.2018.04.003CrossRef

10.

Mistri, A., Dhami, N., Bhattacharyya, S.K., Barai, S.V., Mukherjee, A.: Biocement treatment for upcycling construction and demolition wastes as concrete aggregates. Mater. Struct. 55(6), 152 (2022). https://doi.org/10.1617/s11527-022-01955-3CrossRef

11.

Bhutange, S.P., Latkar, M.V., Muhammad, S.: A review on the potential challenges in the application of biocementation in cement-based materials, possible solutions and way forward. Mater. Today Commun. 107986 (2023). https://doi.org/10.1016/j.mtcomm.2023.107986

12.

Bureau of Indian Standard IS 10262: Concrete Mix Proportioning-Guidelines, New Delhi, India (2019)

13.

Nedunuri, A.S.S.S., yar Mohammed, A., Muhammad, S.: Carbonation potential of concrete debris fines and its valorisation through mineral carbonation. Constr. Build. Mater. 310, 125162 (2021). https://doi.org/10.1016/j.conbuildmat.2021.125162

14.

Bureau of Indian Standard IS 12269: Ordinary portland cement, 53 grade — specification, New Delhi, India (2013)

15.

Martirena, F., Castaño, T., Alujas, A., Orozco-Morales, R., Martinez, L., Linsel, S.: Improving quality of coarse recycled aggregates through cement coating. J. Sustain. Cem. Based Mater. 6(1), 69–84 (2017). https://doi.org/10.1080/21650373.2016.1234983CrossRef

16.

Li, J., Xiao, H., Zhou, Y.: Influence of coating recycled aggregate surface with pozzolanic powder on properties of recycled aggregate concrete. Constr. Build. Mater. 23(3), 1287–1291 (2009). https://doi.org/10.1016/j.conbuildmat.2008.07.019CrossRef

17.

Wong, H.S., Head, M.K., Buenfeld, N.R.: Pore segmentation of cement-based materials from backscattered electron images. Cem. Concr. Res. 36(6), 1083–1090 (2006). https://doi.org/10.1016/j.cemconres.2005.10.006CrossRef

18.

Bureau of Indian Standard IS 2386 (part 3): Methods of tests for aggregates, New Delhi, India (2021)

Title: Valorisation of Recycled Concrete Aggregates via Calcium Carbonate Precipitation Through Urease Enzyme Extracted from Watermelon Seeds
Authors: Snigdha Bhutange
Salman Muhammad
Publisher: Springer Nature Switzerland
Book: Proceedings of the RILEM Spring Convention and Conference 2024
Print ISBN: 978-3-031-70276-1

Electronic ISBN: 978-3-031-70277-8

Copyright Year: 2025
DOI: https://doi.org/10.1007/978-3-031-70277-8_19

Springer Professional

Abstract

Please log in to get access to your license.

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Springer Professional "Technik"

Springer Professional "Wirtschaft"