nach oben

Erschienen in:

2014 | OriginalPaper | Buchkapitel

15. Engineering Aptamers for Biomedical Applications: Part I

verfasst von : Ya Cao, Genxi Li

Erschienen in: Engineering in Translational Medicine

Verlag: Springer London

Einloggen

Aktivieren Sie unsere intelligente Suche, um passende Fachinhalte oder Patente zu finden.

search-config

KI-gestützte Suche

Aus

Abstract

Aptamers are single-stranded DNA or RNA oligonucleotides that are selected for specific binding to a wide range of targets by systematic evolution of ligands by exponential enrichment (SELEX) technology. Aptamers have high specificity and affinity toward target molecules and exhibit desired thermal stability. Additionally, the oligonucleotide nature makes aptamers easy to be chemically modified or incorporated with other DNA/RNA molecules. Owing to these outstanding properties, aptamers have attracted considerable attention within different branches of biomedicine. On the other hand, biosensors are miniaturized analytical devices that are playing an important role in biomedical applications, especially in clinical diagnoses. Recent advances in molecular engineering of aptamers with enhanced bioavailability signal generation and amplification abilities have greatly facilitated the development of aptamer-based biosensors and have pushed them closer to clinical applications. In this chapter, we will detail the recent development in engineering aptamers and highlight the work for sensor applications by using engineered aptamers.

Sie haben noch keine Lizenz? Dann Informieren Sie sich jetzt über unsere Produkte:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

über 102.000 Bücher
über 537 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Finance + Banking
Management + Führung
Marketing + Vertrieb
Maschinenbau + Werkstoffe
Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

Jetzt informieren

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

über 67.000 Bücher
über 390 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Maschinenbau + Werkstoffe

Jetzt Wissensvorsprung sichern!

Jetzt informieren

Vorheriges Kapitel Engineering Multivalent and Multispecific Protein Therapeutics

Nächstes Kapitel Engineering Aptamers for Biomedical Applications: Part II

Navani N, Li Y (2006) Nucleic acid aptamers and enzymes as sensors. Curr Opin Chem Biol 10(3):272–281. doi:10.1016/j.cbpa.2006.04.003

Ellington AD, Szostak JW (1990) In vitro selection of RNA molecules that bind specific ligands. Nature 346(6287):818–822. doi:10.1038/346818a0

Tuerk C, Gold L (1990) Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. Science 249(4968):505–510. doi:10.1126/science.2200121

Ciesiolka J, Gorski J, Yarus M (1995) Selection of an RNA domain that binds Zn2+. RNA 1(5):538–550

Li N, Wang Y, Pothukuchy A, Syrett A, Husain N, Gopalakrisha S, Kosaraju P, Ellington AD (2008) Aptamers that recognize drug-resistant HIV-1 reverse transcriptase. Nucleic Acids Res 36(21):6739–6751. doi:10.1093/nar/gkn775

Shangguan D, Meng L, Cao ZC, Xiao Z, Fang X, Li Y, Cardona D, Witek RP, Liu C, Tan W (2008) Identification of liver cancer-specific aptamers using whole live cells. Anal Chem 80(3):721–728. doi:10.1021/ac701962v

Mairal T, Cengiz Özalp V, Lozano Sánchez P, Mir M, Katakis I, O’Sullivan CK (2007) Aptamers: molecular tools for analytical applications. Anal Bioanal Chem 390(4):989–1007. doi:10.1007/s00216-007-1346-4

Geiger A, Burgstaller P, von der Eltz H, Roeder A, Famulok M (1996) RNA aptamers that bind l-arginine with sub-micromolar dissociation constants and high enantioselectivity. Nucleic Acids Res 24(6):1029–1036. doi:10.1093/nar/24.6.1029

Hong P, Li W, Li J (2012) Applications of aptasensors in clinical diagnostics. Sensors 12(12):1181–1193. doi:10.3390/s120201181

10.

Tan W, Wang H, Chen Y, Zhang X, Zhu H, Yang C, Yang R, Liu C (2011) Molecular aptamers for drug delivery. Trends Biotechnol 29(12):634–640. doi:10.1016/j.tibtech.2011.06.009

11.

Iliuk AB, Hu L, Tao WA (2011) Aptamer in bioanalytical applications. Anal Chem 83(12):4440–4452. doi:10.1021/ac201057w

12.

Keefe AD, Pai S, Ellington A (2010) Aptamers as therapeutics. Nat Rev Drug Discov 9(7):537–550. doi:10.1038/nrd3141

13.

Meyer C, Hahn U, Rentmeister A (2011) Cell-specific aptamers as emerging therapeutics. J Nucleic Acids 2011:1–18. doi:10.4061/2011/904750

14.

Zhu G, Ye M, Donovan MJ, Song E, Zhao Z, Tan W (2012) Nucleic acid aptamers: an emerging frontier in cancer therapy. Chem Commun 48(85):10472. doi:10.1039/c2cc35042d

15.

Esposito CL, Catuogno S, de Franciscis V, Cerchia L (2011) New insight into clinical development of nucleic acid aptamers. Discov Med 11(61):487–496

16.

Wang J, Li G (2011) Aptamers against cell surface receptors: selection, modification and application. Curr Med Chem 18(27):4107–4116. doi:10.2174/092986711797189628

17.

Hirsch IB, Armstrong D, Bergenstal RM, Buckingham B, Childs BP, Clarke WL, Peters A, Wolpert H (2008) Clinical application of emerging sensor technologies in diabetes management: consensus guidelines for continuous glucose monitoring (CGM). Diabetes Technol Ther 10(4):232–244. doi:10.1089/dia.2008.0016 quiz 245–236

18.

Deisingh AK (2006) Aptamer-based biosensors: biomedical applications. Handb Exp Pharmacol 173:341–357. doi:10.1007/3-540-27262-3_17

19.

Citartan M, Gopinath SC, Tominaga J, Tan SC, Tang TH (2012) Assays for aptamer-based platforms. Biosens Bioelectron 34(1):1–11. doi:10.1016/j.bios.2012.01.002

20.

Vallée-Bélisle A, Plaxco KW (2010) Structure-switching biosensors: inspired by nature. Curr Opin Struc Biol 20(4):518–526. doi:10.1016/j.sbi.2010.05.001

21.

Lubin AA, Plaxco KW (2010) Folding-based electrochemical biosensors: the case for responsive nucleic acid architectures. Acc Chem Res 43(4):496–505. doi:10.1021/ar900165x

22.

You M, Chen Y, Peng L, Han D, Yin B, Ye B, Tan W (2011) Engineering DNA aptamers for novel analytical and biomedical applications. Chem Sci 2(6):1003. doi:10.1039/c0sc00647e

23.

Song K-M, Lee S, Ban C (2012) Aptamers and their biological applications. Sensors 12(12):612–631. doi:10.3390/s120100612

24.

Liu J, Cao Z, Lu Y (2009) Functional nucleic acid sensors. Chem Rev 109(5):1948–1998. doi:10.1021/cr030183i

25.

Tombelli S, Minunni M, Mascini M (2005) Analytical applications of aptamers. Biosens Bioelectron 20(12):2424–2434. doi:10.1016/j.bios.2004.11.006

26.

Wang W, Jia L-Y (2009) Progress in aptamer screening methods. Chinese J Anal Chem 37(3):454–460. doi:10.1016/s1872-2040(08)60092-4

27.

Gopinath SCB (2006) Methods developed for SELEX. Anal Bioanal Chem 387(1):171–182. doi:10.1007/s00216-006-0826-2

28.

Mendonsa SD, Bowser MT (2004) In vitro evolution of functional DNA using capillary electrophoresis. J Am Chem Soc 126(1):20–21. doi:10.1021/ja037832s

29.

Liu Y, Wang C, Li F, Shen S, Tyrrell DLJ, Le XC, Li X-F (2012) DNase-mediated single-cycle selection of aptamers for proteins blotted on a membrane. Anal Chem 84(18):7603–7606. doi:10.1021/ac302047e

30.

Misono TS, Kumar PKR (2005) Selection of RNA aptamers against human influenza virus hemagglutinin using surface plasmon resonance. Anal Biochem 342(2):312–317. doi:10.1016/j.ab.2005.04.013

31.

Miyachi Y, Shimizu N, Ogino C, Kondo A (2009) Selection of DNA aptamers using atomic force microscopy. Nucleic Acids Res 38(4):e21–e21. doi:10.1093/nar/gkp1101

32.

Mendonsa SD, Bowser MT (2005) In vitro selection of aptamers with affinity for neuropeptide Y using capillary electrophoresis. J Am Chem Soc 127(26):9382–9383. doi:10.1021/ja052406n

33.

Tang J, Xie J, Shao N, Yan Y (2006) The DNA aptamers that specifically recognize ricin toxin are selected by two in vitro selection methods. Electrophoresis 27(7):1303–1311. doi:10.1002/elps.200500489

34.

Lou X, Qian J, Xiao Y, Viel L, Gerdon AE, Lagally ET, Atzberger P, Tarasow TM, Heeger AJ, Soh HT (2009) Micromagnetic selection of aptamers in microfluidic channels. Proc Natl Acad Sci USA 106(9):2989–2994. doi:10.1073/pnas.0813135106

35.

Weng C-H, Huang C-J, Lee G-B (2012) Screening of aptamers on microfluidic systems for clinical applications. Sensors 12(12):9514–9529. doi:10.3390/s120709514

36.

Qian J, Lou X, Zhang Y, Xiao Y, Soh HT (2009) Generation of highly specific aptamers via micromagnetic selection. Anal Chem 81(13):5490–5495. doi:10.1021/ac900759k

37.

Fang X, Tan W (2010) Aptamers generated from cell-SELEX for molecular medicine: a chemical biology approach. Acc Chem Res 43(1):48–57. doi:10.1021/ar900101s

38.

Shangguan D, Li Y, Tang Z, Cao ZC, Chen HW, Mallikaratchy P, Sefah K, Yang CJ, Tan W (2006) From the cover: aptamers evolved from live cells as effective molecular probes for cancer study. Proc Natl Acad Sci USA 103(32):11838–11843. doi:10.1073/pnas.0602615103

39.

Tang Z, Parekh P, Turner P, Moyer RW, Tan W (2009) Generating aptamers for recognition of virus-infected cells. Clin Chem 55(4):813–822. doi:10.1373/clinchem.2008.113514

40.

Kunii T, S-i Ogura, Mie M, Kobatake E (2011) Selection of DNA aptamers recognizing small cell lung cancer using living cell-SELEX. Analyst 136(7):1310. doi:10.1039/c0an00962h

41.

Liu J, Liu H, Sefah K, Liu B, Pu Y, Van Simaeys D, Tan W (2012) Selection of aptamers specific for adipose tissue. PLoS ONE 7(5):e37789. doi:10.1371/journal.pone.0037789

42.

Thielges MC, Zimmermann J, Yu W, Oda M, Romesberg FE (2008) Exploring the energy landscape of antibody-antigen complexes: protein dynamics, flexibility, and molecular recognition. Biochemistry 47(27):7237–7247. doi:10.1021/bi800374q

43.

Liu L, Zhu X, Zhang D, Huang J, Li G (2007) An electrochemical method to detect folate receptor positive tumor cells. Electrochem Commun 9(10):2547–2550. doi:10.1016/j.elecom.2007.07.032

44.

Cao Y, Zhu S, Yu J, Zhu X, Yin Y, Li G (2012) Protein detection based on small molecule-linked DNA. Anal Chem 84(10):4314–4320. doi:10.1021/ac203401h

45.

Wang J, Shen M, Cao Y, Li G (2010) Switchable “on–off” electrochemical technique for detection of phosphorylation. Biosens Bioelectron 26(2):638–642. doi:10.1016/j.bios.2010.07.006

46.

Xu Y, Cheng G, He P, Fang Y (2009) A review: electrochemical aptasensors with various detection strategies. Electroanalysis 21(11):1251–1259. doi:10.1002/elan.200804561

47.

Tolle F, Mayer G (2013) Dressed for success—applying chemistry to modulate aptamer functionality. Chem Sci 4(1):60. doi:10.1039/c2sc21510a

48.

Burmeister PE, Lewis SD, Silva RF, Preiss JR, Horwitz LR, Pendergrast PS, McCauley TG, Kurz JC, Epstein DM, Wilson C, Keefe AD (2005) Direct in vitro selection of a 2′-O-methyl aptamer to VEGF. Chem Biol 12(1):25–33. doi:10.1016/j.chembiol.2004.10.017

49.

Sekiya S, Noda K, Nishikawa F, Yokoyama T, Kumar PK, Nishikawa S (2006) Characterization and application of a novel RNA aptamer against the mouse prion protein. J Biochem 139(3):383–390. doi:10.1093/jb/mvj046

50.

Lin Y, Nieuwlandt D, Magallanez A, Feistner B, Jayasena SD (1996) High-affinity and specific recognition of human thyroid stimulating hormone (hTSH) by in vitro-selected 2′-amino-modified RNA. Nucleic Acids Res 24(17):3407–3414. doi:10.1093/nar/24.17.3407

51.

Virno A, Randazzo A, Giancola C, Bucci M, Cirino G, Mayol L (2007) A novel thrombin binding aptamer containing a G-LNA residue. Bioorgan Med Chem 15(17):5710–5718. doi:10.1016/j.bmc.2007.06.008

52.

Schmidt KS (2004) Application of locked nucleic acids to improve aptamer in vivo stability and targeting function. Nucleic Acids Res 32(19):5757–5765. doi:10.1093/nar/gkh862

53.

Kuwahara M, Sugimoto N (2010) Molecular evolution of functional nucleic acids with chemical modifications. Molecules 15(8):5423–5444. doi:10.3390/molecules15085423

54.

Shoji A, Kuwahara M, Ozaki H, Sawai H (2007) Modified DNA aptamer that binds the (R)-isomer of a thalidomide derivative with high enantioselectivity. J Am Chem Soc 129(5):1456–1464. doi:10.1021/ja067098n

55.

Keefe AD, Cload ST (2008) SELEX with modified nucleotides. Curr Opin Chem Biol 12(4):448–456. doi:10.1016/j.cbpa.2008.06.028

56.

King DJ, Bassett SE, Li X, Fennewald SA, Herzog NK, Luxon BA, Shope R, Gorenstein DG (2002) Combinatorial selection and binding of phosphorothioate aptamers targeting human NF-kappa B RelA(p65) and p50. Biochemistry 41(30):9696–9706. doi:10.1021/bi020220k

57.

King DJ, Ventura DA, Brasier AR, Gorenstein DG (1998) Novel combinatorial selection of phosphorothioate oligonucleotide aptamers. Biochemistry 37(47):16489–16493. doi:10.1021/bi981780f

58.

Du H, Rosbash M (2002) The U1 snRNP protein U1C recognizes the 5′splice site in the absence of base pairing. Nature 419(6902):86–90. doi:10.1038/nature00947

59.

Pagratis NC, Bell C, Chang YF, Jennings S, Fitzwater T, Jellinek D, Dang C (1997) Potent 2′-amino-, and 2′-fluoro-2′-deoxyribonucleotide RNA inhibitors of keratinocyte growth factor. Nat Biotechnol 15(1):68–73. doi:10.1038/nbt0197-68

60.

Musumeci D, Montesarchio D (2012) Polyvalent nucleic acid aptamers and modulation of their activity: a focus on the thrombin binding aptamer. Pharmacol Therapeut 136(2):202–215. doi:10.1016/j.pharmthera.2012.07.011

61.

Dollins CM, Nair S, Boczkowski D, Lee J, Layzer JM, Gilboa E, Sullenger BA (2008) Assembling OX40 aptamers on a molecular scaffold to create a receptor-activating aptamer. Chem Biol 15(7):675–682. doi:10.1016/j.chembiol.2008.05.016

62.

Tasset DM, Kubik MF, Steiner W (1997) Oligonucleotide inhibitors of human thrombin that bind distinct epitopes. J Mol Biol 272(5):688–698. doi:10.1006/jmbi.1997.1275

63.

Padmanabhan K, Padmanabhan KP, Ferrara JD, Sadler JE, Tulinsky A (1993) The structure of alpha-thrombin inhibited by a 15-mer single-stranded DNA aptamer. J Biol Chem 268(24):17651–17654

64.

Bock LC, Griffin LC, Latham JA, Vermaas EH, Toole JJ (1992) Selection of single-stranded DNA molecules that bind and inhibit human thrombin. Nature 355(6360):564–566. doi:10.1038/355564a0

65.

Müller J, Wulffen B, Pötzsch B, Mayer G (2007) Multidomain targeting generates a high-affinity thrombin-inhibiting bivalent aptamer. ChemBioChem 8(18):2223–2226. doi:10.1002/cbic.200700535

66.

Kim Y, Dennis DM, Morey T, Yang L, Tan W (2010) Engineering dendritic aptamer assemblies as superior inhibitors of protein function. Chem Asian J 5(1):56–59. doi:10.1002/asia.200900421

67.

Hasegawa H, Taira K, Sode K, Ikebukuro K (2008) Improvement of aptamer affinity by dimerization. Sensors 8:1090–1098. doi:10.3390/s8021090

68.

Hsu C-L, Chang H-T, Chen C-T, Wei S-C, Shiang Y-C, Huang C–C (2011) Highly efficient control of thrombin activity by multivalent nanoparticles. Chem Eur J 17(39):10994–11000. doi:10.1002/chem.201101081

69.

Huang YF, Chang HT, Tan W (2008) Cancer cell targeting using multiple aptamers conjugated on nanorods. Anal Chem 80(3):567–572. doi:10.1021/ac702322j

70.

Wang Y, Li Z, Hu D, Lin CT, Li J, Lin Y (2010) Aptamer/graphene oxide nanocomplex for in situ molecular probing in living cells. J Am Chem Soc 132(27):9274–9276. doi:10.1021/ja103169v

71.

Cho H, Baker BR, Wachsmann-Hogiu S, Pagba CV, Laurence TA, Lane SM, Lee LP, Tok JB (2008) Aptamer-based SERRS sensor for thrombin detection. Nano Lett 8(12):4386–4390. doi:10.1021/nl802245w

72.

Xing H, Wong NY, Xiang Y, Lu Y (2012) DNA aptamer functionalized nanomaterials for intracellular analysis, cancer cell imaging and drug delivery. Curr Opin Chem Biol 16(3–4):429–435. doi:10.1016/j.cbpa.2012.03.016

73.

Tyagi S, Kramer FR (1996) Molecular beacons: probes that fluoresce upon hybridization. Nat Biotechnol 14(3):303–308. doi:10.1038/nbt0396-303

74.

Tan W, Fang X, Li J, Liu X (2000) Molecular beacons: a novel DNA probe for nucleic acid and protein studies. Chemistry 6(7):1107–1111. doi:10.1002/(SICI)1521-3765(20000403)6:7<1107:AID-CHEM1107>3.0.CO;2-9

75.

Wang K, Tang Z, Yang CJ, Kim Y, Fang X, Li W, Wu Y, Medley CD, Cao Z, Li J, Colon P, Lin H, Tan W (2009) Molecular engineering of DNA: molecular beacons. Angew Chem Int Ed 48(5):856–870. doi:10.1002/anie.200800370

76.

Song S, Liang Z, Zhang J, Wang L, Li G, Fan C (2009) Gold-nanoparticle-based multicolor nanobeacons for sequence-specific DNA analysis. Angew Chem Int Ed 48(46):8670–8674. doi:10.1002/anie.200901887

77.

Hamaguchi N, Ellington A, Stanton M (2001) Aptamer beacons for the direct detection of proteins. Anal Biochem 294(2):126–131. doi:10.1006/abio.2001.5169

78.

Kim B, Jung IH, Kang M, Shim H-K, Woo HY (2012) Cationic conjugated polyelectrolytes-triggered conformational change of molecular beacon aptamer for highly sensitive and selective potassium ion detection. J Am Chem Soc 134(6):3133–3138. doi:10.1021/ja210360v

79.

Wu Z-S, Zheng F, Shen G-L, Yu R-Q (2009) A hairpin aptamer-based electrochemical biosensing platform for the sensitive detection of proteins. Biomaterials 30(15):2950–2955. doi:10.1016/j.biomaterials.2009.02.017

80.

Liu Y, Tuleouva N, Ramanculov E, Revzin A (2010) Aptamer-based electrochemical biosensor for interferon gamma detection. Anal Chem 82(19):8131–8136. doi:10.1021/ac101409t

81.

Shi H, He X, Wang K, Wu X, Ye X, Guo Q, Tan W, Qing Z, Yang X, Zhou B (2011) Activatable aptamer probe for contrast-enhanced in vivo cancer imaging based on cell membrane protein-triggered conformation alteration. Proc Natl Acad Sci USA 108(10):3900–3905. doi:10.1073/pnas.1016197108

82.

Zheng J, Li J, Jiang Y, Jin J, Wang K, Yang R, Tan W (2011) Design of aptamer-based sensing platform using triple-helix molecular switch. Anal Chem 83(17):6586–6592. doi:10.1021/ac201314y

83.

Lu Y, Liu J (2006) Functional DNA nanotechnology: emerging applications of DNAzymes and aptamers. Curr Opin Biotech 17(6):580–588. doi:10.1016/j.copbio.2006.10.004

84.

Cho EJ, Yang L, Levy M, Ellington AD (2005) Using a deoxyribozyme ligase and rolling circle amplification to detect a non-nucleic acid analyte, ATP. J Am Chem Soc 127(7):2022–2023. doi:10.1021/ja043490u

85.

Lu L-M, Zhang X-B, Kong R-M, Yang B, Tan W (2011) A ligation-triggered DNAzyme cascade for amplified fluorescence detection of biological small molecules with zero-background signal. J Am Chem Soc 133(30):11686–11691. doi:10.1021/ja203693b

86.

Achenbach JC, Nutiu R, Li Y (2005) Structure-switching allosteric deoxyribozymes. Anal Chim Acta 534(1):41–51. doi:10.1016/j.aca.2004.03.080

87.

Song P, Xiang Y, Xing H, Zhou Z, Tong A, Lu Y (2012) Label-free catalytic and molecular beacon containing an abasic site for sensitive fluorescent detection of small inorganic and organic molecules. Anal Chem 84(6):2916–2922. doi:10.1021/ac203488p

88.

Sook Bang G, Cho S, Lee N, Lee B-R, Kim J-H, Kim B-G (2013) Rational design of modular allosteric aptamer sensor for label-free protein detection. Biosens Bioelectron 39(1):44–50. doi:10.1016/j.bios.2012.06.038

89.

Yoshida W, Sode K, Ikebukuro K (2006) Aptameric enzyme subunit for biosensing based on enzymatic activity measurement. Anal Chem 78(10):3296–3303. doi:10.1021/ac060254o

90.

Stojanovic MN, Kolpashchikov DM (2004) Modular aptameric sensors. J Am Chem Soc 126(30):9266–9270. doi:10.1021/ja032013t

91.

Wang J, Cao Y, Chen G, Li G (2009) Regulation of thrombin activity with a bifunctional aptamer and hemin: development of a new anticoagulant and antidote pair. ChemBioChem 10(13):2171–2176. doi:10.1002/cbic.200900408

92.

Zhang K, Zhu X, Wang J, Xu L, Li G (2010) Strategy to fabricate an electrochemical aptasensor: application to the assay of adenosine deaminase activity. Anal Chem 82(8):3207–3211. doi:10.1021/ac902771k

93.

Wang J, Liu B (2009) Fluorescence resonance energy transfer between an anionic conjugated polymer and a dye-labeled lysozyme aptamer for specific lysozyme detection. Chem Commun 17:2284. doi:10.1039/b820001g

94.

Zuo X, Song S, Zhang J, Pan D, Wang L, Fan C (2007) A target-responsive electrochemical aptamer switch (TREAS) for reagentless detection of nanomolar ATP. J Am Chem Soc 129(5):1042–1043. doi:10.1021/ja067024b

95.

Shlyahovsky B, Li D, Katz E, Willner I (2007) Proteins modified with DNAzymes or aptamers act as biosensors or biosensor labels. Biosens Bioelectron 22(11):2570–2576. doi:10.1016/j.bios.2006.10.009

96.

Chen Y, O’Donoghue MB, Huang YF, Kang H, Phillips JA, Chen X, Estevez MC, Yang CJ, Tan W (2010) A surface energy transfer nanoruler for measuring binding site distances on live cell surfaces. J Am Chem Soc 132(46):16559–16570. doi:10.1021/ja106360v

97.

Drolet DW, Moon-McDermott L, Romig TS (1996) An enzyme-linked oligonucleotide assay. Nat Biotechnol 14(8):1021–1025. doi:10.1038/nbt0896-1021

98.

Green LS, Jellinek D, Jenison R, Ostman A, Heldin CH, Janjic N (1996) Inhibitory DNA ligands to platelet-derived growth factor B-chain. Biochemistry 35(45):14413–14424. doi:10.1021/bi961544+

99.

Pavlov V, Xiao Y, Shlyahovsky B, Willner I (2004) Aptamer-functionalized Au nanoparticles for the amplified optical detection of thrombin. J Am Chem Soc 126(38):11768–11769. doi:10.1021/ja046970u

100.

Song S, Wang L, Li J, Fan C, Zhao J (2008) Aptamer-based biosensors. TrAC-Trend. Anal Chem 27(2):108–117. doi:10.1016/j.trac.2007.12.004

101.

Han K, Liang Z, Zhou N (2010) Design strategies for aptamer-based biosensors. Sensors 10(5):4541–4557. doi:10.3390/s100504541

102.

Mascini M, Palchetti I, Tombelli S (2012) Nucleic acid and peptide aptamers: fundamentals and bioanalytical aspects. Angew Chem Int Ed 51(6):1316–1332. doi:10.1002/anie.201006630

103.

Numnuam A, Chumbimuni-Torres KY, Xiang Y, Bash R, Thavarungkul P, Kanatharana P, Pretsch E, Wang J, Bakker E (2008) Aptamer-based potentiometric measurements of proteins using ion-selective microelectrodes. Anal Chem 80(3):707–712. doi:10.1021/ac701910r

104.

Wang J, Meng W, Zheng X, Liu S, Li G (2009) Combination of aptamer with gold nanoparticles for electrochemical signal amplification: application to sensitive detection of platelet-derived growth factor. Biosens Bioelectron 24(6):1598–1602. doi:10.1016/j.bios.2008.08.030

105.

Bai L, Yuan R, Chai Y, Zhuo Y, Yuan Y, Wang Y (2012) Simultaneous electrochemical detection of multiple analytes based on dual signal amplification of single-walled carbon nanotubes and multi-labeled graphene sheets. Biomaterials 33(4):1090–1096. doi:10.1016/j.biomaterials.2011.10.012

106.

Zhang YL, Huang Y, Jiang JH, Shen GL, Yu RQ (2007) Electrochemical aptasensor based on proximity-dependent surface hybridization assay for single-step, reusable, sensitive protein detection. J Am Chem Soc 129(50):15448–15449. doi:10.1021/ja0773047

107.

Wang J, Munir A, Li Z, Zhou HS (2009) Aptamer–Au NPs conjugates-enhanced SPR sensing for the ultrasensitive sandwich immunoassay. Biosens Bioelectron 25(1):124–129. doi:10.1016/j.bios.2009.06.016

108.

Yang CJ, Jockusch S, Vicens M, Turro NJ, Tan WH (2005) Light-switching excimer probes for rapid protein monitoring in complex biological fluids. Proc Natl Acad Sci USA 102(48):17278–17283. doi:10.1073/pnas.0508821102

109.

Mok W, Li Y (2008) Recent progress in nucleic acid aptamer-based biosensors and bioassays. Sensors 8(11):7050–7084. doi:10.3390/s8117050

110.

Willner I, Zayats M (2007) Electronic aptamer-based sensors. Angew Chem Int Ed 46(34):6408–6418. doi:10.1002/anie.200604524

111.

Xiao Y, Lubin AA, Heeger AJ, Plaxco KW (2005) Label-free electronic detection of thrombin in blood serum by using an aptamer-based sensor. Angew Chem Int Ed 44(34):5456–5459. doi:10.1002/anie.200500989

112.

Wu Z-S, Chen C-R, Shen G-L, Yu R-Q (2008) Reversible electronic nanoswitch based on DNA G-quadruplex conformation: a platform for single-step, reagentless potassium detection. Biomaterials 29(17):2689–2696. doi:10.1016/j.biomaterials.2008.02.024

113.

Li H, Cao Y, Wu X, Ye Z, Li G (2012) Peptide-based electrochemical biosensor for amyloid β 1–42 soluble oligomer assay. Talanta 93:358–363. doi:10.1016/j.talanta.2012.02.055

114.

White RJ, Plaxco KW (2010) Exploiting binding-induced changes in probe flexibility for the optimization of electrochemical biosensors. Anal Chem 82(1):73–76. doi:10.1021/ac902595f

115.

Ferapontova EE, Olsen EM, Gothelf KV (2008) An RNA aptamer-based electrochemical biosensor for detection of theophylline in serum. J Am Chem Soc 130(13):4256–4258. doi:10.1021/ja711326b

116.

Radi AE, Acero Sanchez JL, Baldrich E, O’Sullivan CK (2006) Reagentless, reusable, ultrasensitive electrochemical molecular beacon aptasensor. J Am Chem Soc 128(1):117–124. doi:10.1021/ja053121d

117.

Baker BR, Lai RY, Wood MS, Doctor EH, Heeger AJ, Plaxco KW (2006) An electronic, aptamer-based small-molecule sensor for the rapid, label-free detection of cocaine in adulterated samples and biological fluids. J Am Chem Soc 128(10):3138–3139. doi:10.1021/ja056957p

118.

Zhao S, Yang W, Lai RY (2011) A folding-based electrochemical aptasensor for detection of vascular endothelial growth factor in human whole blood. Biosens Bioelectron 26(5):2442–2447. doi:10.1016/j.bios.2010.10.029

119.

Radi A-E, O’Sullivan CK (2006) Aptamer conformational switch as sensitive electrochemical biosensor for potassium ion recognition. Chem Commun 32:3432. doi:10.1039/b606804a

120.

Zuo X, Xiao Y, Plaxco KW (2009) High specificity, electrochemical sandwich assays based on single aptamer sequences and suitable for the direct detection of small-molecule targets in blood and other complex matrices. J Am Chem Soc 131(20):6944–6945. doi:10.1021/ja901315w

121.

Sharma AK, Kent AD, Heemstra JM (2012) Enzyme-linked small-molecule detection using split aptamer ligation. Anal Chem 84(14):6104–6109. doi:10.1021/ac300997q

122.

Freeman R, Sharon E, Tel-Vered R, Willner I (2009) Supramolecular cocaine-aptamer complexes activate biocatalytic cascades. J Am Chem Soc 131(14):5028–5029. doi:10.1021/ja809496n

123.

Kashefi-Kheyrabadi L, Mehrgardi MA (2012) Aptamer-conjugated silver nanoparticles for electrochemical detection of adenosine triphosphate. Biosens Bioelectron 37(1):94–98. doi:10.1016/j.bios.2012.04.045

124.

Dave N, Liu J (2012) Biomimetic sensing based on chemically induced assembly of a signaling DNA aptamer on a fluid bilayer membrane. Chem Commun 48(31):3718. doi:10.1039/c2cc00070a

125.

Freeman R, Girsh J, Fang-ju Jou A, Ho JAA, Hug T, Dernedde J, Willner I (2012) Optical aptasensors for the analysis of the vascular endothelial growth factor (VEGF). Anal Chem 84(14):6192–6198. doi:10.1021/ac3011473

126.

Yamamoto-Fujita R, Kumar PK (2005) Aptamer-derived nucleic acid oligos: applications to develop nucleic acid chips to analyze proteins and small ligands. Anal Chem 77(17):5460–5466. doi:10.1021/ac050364f

127.

Lin Z, Chen L, Zhu X, Qiu B, Chen G (2010) Signal-on electrochemiluminescence biosensor for thrombin based on target-induced conjunction of split aptamer fragments. Chem Commun 46(30):5563. doi:10.1039/c0cc00932f

128.

Nutiu R, Li Y (2003) Structure-switching signaling aptamers. J Am Chem Soc 125(16):4771–4778. doi:10.1021/ja028962o

129.

Mei H, Bing T, Yang X, Qi C, Chang T, Liu X, Cao Z, Shangguan D (2012) Functional-group specific aptamers indirectly recognizing compounds with alkyl amino group. Anal Chem 84(17):7323–7329. doi:10.1021/ac300281u

130.

Zhang J, Wang L, Zhang H, Boey F, Song S, Fan C (2010) Aptamer-based multicolor fluorescent gold nanoprobes for multiplex detection in homogeneous solution. Small 6(2):201–204. doi:10.1002/smll.200901012

131.

Yoshizumi J, Kumamoto S, Nakamura M, Yamana K (2008) Target-induced strand release (TISR) from aptamer–DNA duplex: a general strategy for electronic detection of biomolecules ranging from a small molecule to a large protein. Analyst 133(3):323. doi:10.1039/b719089c

132.

Wu ZS, Guo MM, Zhang SB, Chen CR, Jiang JH, Shen GL, Yu RQ (2007) Reusable electrochemical sensing platform for highly sensitive detection of small molecules based on structure-switching signaling aptamers. Anal Chem 79(7):2933–2939. doi:10.1021/ac0622936

133.

Chen L, Cai Q, Luo F, Chen X, Zhu X, Qiu B, Lin Z, Chen G (2010) A sensitive aptasensor for adenosine based on the quenching of Ru(bpy)32+-doped silica nanoparticle ECL by ferrocene. Chem Commun 46(41):7751. doi:10.1039/c0cc03225e

134.

Li H, Rothberg L (2004) Colorimetric detection of DNA sequences based on electrostatic interactions with unmodified gold nanoparticles. Proc Natl Acad Sci USA 101(39):14036–14039. doi:10.1073/pnas.0406115101

135.

Zheng M, Jagota A, Semke ED, Diner BA, McLean RS, Lustig SR, Richardson RE, Tassi NG (2003) DNA-assisted dispersion and separation of carbon nanotubes. Nat Mate 2(5):338–342. doi:10.1038/nmat877

136.

Lu C-H, Yang H–H, Zhu C-L, Chen X, Chen G-N (2009) A graphene platform for sensing biomolecules. Angew Chem Int Ed 48(26):4785–4787. doi:10.1002/anie.200901479

137.

Yang R, Tang Z, Yan J, Kang H, Kim Y, Zhu Z, Tan W (2008) Noncovalent assembly of carbon nanotubes and single-stranded DNA: an effective sensing platform for probing biomolecular interactions. Anal Chem 80(19):7408–7413. doi:10.1021/ac801118p

138.

Zhao J, Chen G, Zhu L, Li G (2011) Graphene quantum dots-based platform for the fabrication of electrochemical biosensors. Electrochem Commun 13(1):31–33. doi:10.1016/j.elecom.2010.11.005

139.

Wang J, Wang L, Liu X, Liang Z, Song S, Li W, Li G, Fan C (2007) A gold nanoparticle-based aptamer target binding readout for ATP assay. Adv Mater 19(22):3943–3946. doi:10.1002/adma.200602256

140.

Wang W, Chen C, Qian M, Zhao XS (2008) Aptamer biosensor for protein detection using gold nanoparticles. Anal Biochem 373(2):213–219. doi:10.1016/j.ab.2007.11.013

141.

Wang L, Zhu J, Han L, Jin L, Zhu C, Wang E, Dong S (2012) Graphene-based aptamer logic gates and their application to multiplex detection. ACS Nano 6(8):6659–6666. doi:10.1021/nn300997f

142.

Zhu Z, Tang Z, Phillips JA, Yang R, Wang H, Tan W (2008) Regulation of singlet oxygen generation using single-walled carbon nanotubes. J Am Chem Soc 130(33):10856–10857. doi:10.1021/ja802913f

143.

Tang D, Tang J, Li Q, Su B, Chen G (2011) Ultrasensitive aptamer-based multiplexed electrochemical detection by coupling distinguishable signal tags with catalytic recycling of DNase I. Anal Chem 83(19):7255–7259. doi:10.1021/ac201891w

144.

Lei J, Ju H (2012) Signal amplification using functional nanomaterials for biosensing. Chem Soc Rev 41(6):2122. doi:10.1039/c1cs15274b

145.

Chiu T-C, Huang C–C (2009) Aptamer-functionalized nano-biosensors. Sensors 9(12):10356–10388. doi:10.3390/s91210356

146.

Moore P (2005) PCR: replicating success. Nature 435(7039):235–238. doi:10.1038/435235a

147.

Kim J, Easley CJ (2011) Isothermal DNA amplification in bioanalysis: strategies and applications. Bioanalysis 3(2):227–239. doi:10.4155/bio.10.172

148.

Eisenstein BI (1990) The polymerase chain reaction. A new method of using molecular genetics for medical diagnosis. N Engl J Med 322(3):178–183. doi:10.1056/NEJM199001183220307

149.

Liao S, Liu Y, Zeng J, Li X, Shao N, Mao A, Wang L, Ma J, Cen H, Wang Y, Zhang X, Zhang R, Wei Z, Wang X (2010) Aptamer-based sensitive detection of target molecules via RT-PCR signal amplification. Bioconjug Chem 21(12):2183–2189. doi:10.1021/bc100032v

150.

Fredriksson S, Gullberg M, Jarvius J, Olsson C, Pietras K, Gustafsdottir SM, Ostman A, Landegren U (2002) Protein detection using proximity-dependent DNA ligation assays. Nat Biotechnol 20(5):473–477. doi:10.1038/nbt0502-473

151.

Pai SS, Ellington AD (2009) Using RNA aptamers and the proximity ligation assay for the detection of cell surface antigens. Methods Mol Biol 504:385–398. doi:10.1007/978-1-60327-569-9_21

152.

Kim J, Hu J, Sollie RS, Easley CJ (2010) Improvement of sensitivity and dynamic range in proximity ligation assays by asymmetric connector hybridization. Anal Chem 82(16):6976–6982. doi:10.1021/ac101762m

153.

Lizardi PM, Huang X, Zhu Z, Bray-Ward P, Thomas DC, Ward DC (1998) Mutation detection and single-molecule counting using isothermal rolling-circle amplification. Nat Genet 19(3):225–232. doi:10.1038/898

154.

Miao P, Ning L, Li X, Li P, Li G (2012) Electrochemical strategy for sensing protein phosphorylation. Bioconjugate Chem 23(1):141–145. doi:10.1021/bc200523p

155.

Zhao W, Ali MM, Brook MA, Li Y (2008) Rolling circle amplification: applications in nanotechnology and biodetection with functional nucleic acids. Angew Chem Int Ed 47(34):6330–6337. doi:10.1002/anie.200705982

156.

Lee J, Icoz K, Roberts A, Ellington AD, Savran CA (2010) Diffractometric detection of proteins using microbead-based rolling circle amplification. Anal Chem 82(1):197–202. doi:10.1021/ac901716d

157.

Cho EJ, Lee J-W, Ellington AD (2009) Applications of aptamers as sensors. Annu Rev Anal Chem 2(1):241–264. doi:10.1146/annurev.anchem.1.031207.112851

158.

Yang L, Fung CW, Cho EJ, Ellington AD (2007) Real-time rolling circle amplification for protein detection. Anal Chem 79(9):3320–3329. doi:10.1021/ac062186b

159.

Xu Y, Lunnen KD, Kong H (2001) Engineering a nicking endonuclease N. AlwI by domain swapping. Proc Natl Acad Sci USA 98(23):12990–12995. doi:10.1073/pnas.241215698

160.

Xu W, Xue X, Li T, Zeng H, Liu X (2009) Ultrasensitive and selective colorimetric DNA detection by nicking endonuclease assisted nanoparticle amplification. Angew Chem Int Ed 48(37):6849–6852. doi:10.1002/anie.200901772

161.

Liu Z, Zhang W, Zhu S, Zhang L, Hu L, Parveen S, Xu G (2011) Ultrasensitive signal-on DNA biosensor based on nicking endonuclease assisted electrochemistry signal amplification. Biosens Bioelectron 29(1):215–218. doi:10.1016/j.bios.2011.07.076

162.

Li J, Fu H-E, Wu L-J, Zheng A-X, Chen G-N, Yang H–H (2012) General colorimetric detection of proteins and small molecules based on cyclic enzymatic signal amplification and hairpin aptamer probe. Anal Chem 84(12):5309–5315. doi:10.1021/ac3006186

163.

Jie G, Wang L, Yuan J, Zhang S (2011) Versatile electrochemiluminescence assays for cancer cells based on dendrimer/CdSe–ZnS–quantum dot nanoclusters. Anal Chem 83(10):3873–3880. doi:10.1021/ac200383z

164.

Zhu X, Zhao J, Wu Y, Shen Z, Li G (2011) Fabrication of a highly sensitive aptasensor for potassium with a nicking endonuclease-assisted signal amplification strategy. Anal Chem 83(11):4085–4089. doi:10.1021/ac200058r

165.

Feng K, Kong R, Wang H, Zhang S, Qu F (2012) A universal amplified strategy for aptasensors: enhancing sensitivity through allostery-triggered enzymatic recycling amplification. Biosens Bioelectron 38(1):121–125. doi:10.1016/j.bios.2012.05.008

166.

Zhang H, Li F, Dever B, Li X-F, Le XC (2012) DNA-mediated homogeneous binding assays for nucleic acids and proteins. Chem Rev. doi:10.1021/cr300340p

167.

Fan Q, Zhao J, Li H, Zhu L, Li G (2012) Exonuclease III-based and gold nanoparticle-assisted DNA detection with dual signal amplification. Biosens Bioelectron 33(1):211–215. doi:10.1016/j.bios.2012.01.003

168.

Zuo X, Xia F, Xiao Y, Plaxco KW (2010) Sensitive and selective amplified fluorescence DNA detection based on exonuclease III-aided target recycling. J Am Chem Soc 132(6):1816–1818. doi:10.1021/ja909551b

169.

Xuan F, Luo X, Hsing IM (2012) Ultrasensitive solution-phase electrochemical molecular beacon-based DNA detection with signal amplification by exonuclease III-assisted target recycling. Anal Chem 84(12):5216–5220. doi:10.1021/ac301033w

170.

Liu X, Freeman R, Willner I (2012) Amplified fluorescence aptamer-based sensors using exonuclease III for the regeneration of the analyte. Chem Eur J 18(8):2207–2211. doi:10.1002/chem.201103342

171.

Tong P, Zhang L, Xu J–J, Chen H-Y (2011) Simply amplified electrochemical aptasensor of ochratoxin A based on exonuclease-catalyzed target recycling. Biosens Bioelectron 29(1):97–101. doi:10.1016/j.bios.2011.07.075

172.

Scrimin P, Prins LJ (2011) Sensing through signal amplification. Chem Soc Rev 40(9):4488. doi:10.1039/c1cs15024c

173.

Zhu X, Yang J, Liu M, Wu Y, Shen Z, Li G (2013) Sensitive detection of human breast cancer cells based on aptamer–cell–aptamer sandwich architecture. Anal Chim Acta 764:59–63. doi:10.1016/j.aca.2012.12.024

174.

Cao Y, Wang J, Xu Y, Li G (2010) Combination of enzyme catalysis and electrocatalysis for biosensor fabrication: Application to assay the activity of indoleamine 2,3-dioxygensae. Biosens Bioelectron 26(1):87–91. doi:10.1016/j.bios.2010.05.019

175.

Song Y, Qu K, Zhao C, Ren J, Qu X (2010) Graphene oxide: intrinsic peroxidase catalytic activity and its application to glucose detection. Adv Mater 22(19):2206–2210. doi:10.1002/adma.200903783

176.

Gao L, Zhuang J, Nie L, Zhang J, Zhang Y, Gu N, Wang T, Feng J, Yang D, Perrett S, Yan X (2007) Intrinsic peroxidase-like activity of ferromagnetic nanoparticles. Nat Nanotechnol 2(9):577–583. doi:10.1038/nnano.2007.260

177.

Golub E, Pelossof G, Freeman R, Zhang H, Willner I (2009) Electrochemical, photoelectrochemical, and surface plasmon resonance detection of cocaine using supramolecular aptamer complexes and metallic or semiconductor nanoparticles. Anal Chem 81(22):9291–9298. doi:10.1021/ac901551q

178.

Li W, Li J, Qiang W, Xu J, Xu D (2013) Enzyme-free colorimetric bioassay based on gold nanoparticle-catalyzed dye decolorization. Analyst 138(3):760. doi:10.1039/c2an36374g

179.

Zhang XB, Wang Z, Xing H, Xiang Y, Lu Y (2010) Catalytic and molecular beacons for amplified detection of metal ions and organic molecules with high sensitivity. Anal Chem 82(12):5005–5011. doi:10.1021/ac1009047

180.

Vinkenborg JL, Karnowski N, Famulok M (2011) Aptamers for allosteric regulation. Nat Chem Biol 7(8):519–527. doi:10.1038/nchembio.609

181.

Yang Q, Nie Y, Zhu X, Liu X, Li G (2009) Study on the electrocatalytic activity of human telomere G-quadruplex–hemin complex and its interaction with small molecular ligands. Electrochim Acta 55(1):276–280. doi:10.1016/j.electacta.2009.08.050

182.

Zhu X, Zhang W, Xiao H, Huang J, Li G (2008) Electrochemical study of a hemin–DNA complex and its activity as a ligand binder. Electrochim Acta 53(13):4407–4413. doi:10.1016/j.electacta.2008.01.042

183.

Pelossof G, Tel-Vered R, Elbaz J, Willner I (2010) Amplified biosensing using the horseradish peroxidase-mimicking DNAzyme as an electrocatalyst. Anal Chem 82(11):4396–4402. doi:10.1021/ac100095u

184.

Golub E, Freeman R, Willner I (2011) A hemin/G-quadruplex acts as an NADH oxidase and NADH peroxidase mimicking DNAzyme. Angew Chem Int Ed 50(49):11710–11714. doi:10.1002/anie.201103853

185.

Tang L, Liu Y, Ali MM, Kang DK, Zhao W, Li J (2012) Colorimetric and ultrasensitive bioassay based on a dual-amplification system using aptamer and DNAzyme. Anal Chem 84(11):4711–4717. doi:10.1021/ac203274k

186.

Yuan Y, Yuan R, Chai Y, Zhuo Y, Ye X, Gan X, Bai L (2012) Hemin/G-quadruplex simultaneously acts as NADH oxidase and HRP-mimicking DNAzyme for simple, sensitive pseudobienzyme electrochemical detection of thrombin. Chem Commun 48(38):4621–4623. doi:10.1039/c2cc31423a

187.

Zhu X, Cao Y, Liang Z, Li G (2010) Aptamer-based and DNAzyme-linked colorimetric detection of cancer cells. Protein Cell 1(9):842–846. doi:10.1007/s13238-010-0110-2

Titel: Engineering Aptamers for Biomedical Applications: Part I
verfasst von: Ya Cao
Genxi Li
Verlag: Springer London
Buch: Engineering in Translational Medicine
Print ISBN: 978-1-4471-4371-0

Electronic ISBN: 978-1-4471-4372-7

Copyright-Jahr: 2014
DOI: https://doi.org/10.1007/978-1-4471-4372-7_15

Neuer Inhalt

Bildnachweise

VDI-Icon, Profil Icon, inhalt2, Springer Professional Modul/© Springer Fachmedien Wiesbaden GmbH, Nachhaltigkeitsaward Key Visual/© Cometis AG/Global ESG Monitor | Daniel Rupp | Generiert mit KI, Search Icon, Banner Hanser, Frank Urbansky/© Peter Eichler / Leipzig, CO2-Fußabdruck/© Jenny Sturm / stock.adobe.com, Interview Entropie Bild 1/© Bernhard Weßling, Zeitschrift Wissensmanagement Cover, PatentFit-Logo/© Springer Fachmedien Wiesbaden GmbH, Sustainibility Finance/© Robert Kneschke / stock.adobe.com / Springer Fachmedien Wiesbaden GmbH, Zukunftswerkstatt Sales Excellence 2024/© AndreyPopov / Getty Images / iStock, 2023_Antrieb/© supervisuell

Springer Professional

Abstract

Bitte loggen Sie sich ein, um Zugang zu Ihrer Lizenz zu erhalten.

Sie haben noch keine Lizenz? Dann Informieren Sie sich jetzt über unsere Produkte:

Springer Professional "Wirtschaft+Technik"

Springer Professional "Technik"

Neuer Inhalt

Bitte loggen Sie sich ein, um Zugang zu Ihrer Lizenz zu erhalten.

Bitte loggen Sie sich ein, um Zugang zu Ihrer Lizenz zu erhalten.