Enhancement of Liposome Delivery Efficiency Via Cell Membrane Permeability by ultrasound exposure

Ultrasound has a number of attractive features as the diagnostic imaging modality and therapeutic modality. Under the clinical application ultrasound irradiation, sonication may be performed noninvasively or minimally invasively. In order to evaluate and characterize the performance of the designed liposome with tumor cell under the ultrasound irradiation, we prepared the FITC-inclusion liposome drug delivery model. The effect of different voltage at 1MHz frequency ultrasound transducer applied to MCF7 cells in the presence of the liposome was evaluated morphologically, using a scanning electron microscope. The delivery efficiency of FITC-inclusion liposome drug delivery model had been quantified using flow cytometry. The ultrasound voltages were 400mv, 600mv, 800mv and 1000mv at the frequency of 1MHZ respectively. Exposure of MCF7 cells to these voltages with liposome resulted in a different FITC absorption. Under the appropriate ultrasound energy guiding, liposome can efficiently delivery drug transiently and nonlethally with increase in cell membrane permeability. In this way, the noninvasively focused ultrasound could deliver drugs, protein and genes to targeted tissues, minimizing side effects, lowering drug dosages and improving efficacy.