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Erschienen in:

2015 | Online First | Buchkapitel

Enhancing Induced Pluripotent Stem Cell Generation by MicroRNA

verfasst von : Jason Dang, Tariq M Rana

Erschienen in: Methods in Molecular Biology™

Verlag: Springer New York

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Abstract

Somatic reprogramming to generate induced pluripotent stem cells, or iPSC, is a powerful tool in developmental biology, disease modeling, and regenerative medicine. microRNAs have been shown to regulate many key pathways in iPSC induction. Here we describe a microRNA mimic enhanced somatic reprogramming process starting from mouse embryonic fibroblast isolation to iPSC induction to colony derivation and characterization.
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Metadaten
Titel
Enhancing Induced Pluripotent Stem Cell Generation by MicroRNA
verfasst von
Jason Dang
Tariq M Rana
Copyright-Jahr
2015
Verlag
Springer New York
DOI
https://doi.org/10.1007/7651_2015_201