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Erschienen in:
2020 | OriginalPaper | Buchkapitel
Estimating Enzyme Participation in Metabolic Pathways for Microbial Communities from RNA-seq Data
verfasst von : F. Rondel, R. Hosseini, B. Sahoo, S. Knyazev, I. Mandric, Frank Stewart, I. I. Măndoiu, B. Pasaniuc, A. Zelikovsky
Erschienen in: Bioinformatics Research and Applications
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Abstract
Metatranscriptome sequence data analysis is necessary for understanding biochemical changes in the microbial community and their effects. In this paper, we propose a methodology to estimate activities of individual metabolic pathways to better understand the activity of the entire metabolic network. Our novel pipeline includes an expectation-maximization based estimation of enzyme expression and simultaneous estimation of pathway activity level and enzyme participation level in each pathway. We applied our novel pipeline to metatranscriptome data generated from surface water planktonic communities sampled over a day-night cycle in the Northern Gulf of Mexico (Louisiana Shelf). Our results show the estimated enzyme expression, pathway activity levels as well as enzyme participation levels in each pathway are robust and stable across all data points. In contrast to expression of enzymes, the estimated activity levels of significant number of metabolic pathways strongly correlate with the environmental parameters.