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Erschienen in:

2014 | OriginalPaper | Buchkapitel

76. Expression, Purification, and Activity Assay of Chicken Interferon-Alpha

verfasst von : Yue Ma, Minhui Long, Aipo Diao

Erschienen in: Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)

Verlag: Springer Berlin Heidelberg

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Abstract

In this paper, Chicken alpha interferon (IFN-α) gene was cloned into pHis-NusA expression vector, then the recombinant expression vector was transformed into host bacteria E. coli BL21. The recombinant chicken IFN-α was induced to express by IPTG, then the protein expression was analyzed with SDS-PAGE. Under the condition that the recombinant protein was induced to express with 0.1 mM IPTG at 16 °C, the expressed protein was soluble. Recombinant Chicken IFN-α was purified by Ni-metal chelate affinity chromatography. The expressed protein was shown to inhibit the replication of Hand foot and mouth disease virus in vero cells.

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Titel: Expression, Purification, and Activity Assay of Chicken Interferon-Alpha
verfasst von: Yue Ma
Minhui Long
Aipo Diao
Verlag: Springer Berlin Heidelberg
Buch: Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)
Print ISBN: 978-3-642-37921-5

Electronic ISBN: 978-3-642-37922-2

Copyright-Jahr: 2014
DOI: https://doi.org/10.1007/978-3-642-37922-2_76

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