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Erschienen in:

2015 | Online First | Buchkapitel

Generation of Embryonic Stem Cells in Rabbits

verfasst von : Marielle Afanassieff, Pierre Osteil, Pierre Savatier

Erschienen in: Methods in Molecular Biology™

Verlag: Springer New York

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Abstract

Here we have described a procedure to generate embryonic stem cell (ESC) lines from rabbit preimplantation blastocysts. We have also provided detailed procedures to characterize the resulting ESC lines, such as the analysis of pluripotency marker expression by reverse transcription quantitative polymerase chain reaction, immunolabeling, and fluorescence-associated cell sorting; evaluation of pluripotency by teratoma production; and assessment of genetic stability by karyotyping.
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Metadaten
Titel
Generation of Embryonic Stem Cells in Rabbits
verfasst von
Marielle Afanassieff
Pierre Osteil
Pierre Savatier
Copyright-Jahr
2015
Verlag
Springer New York
DOI
https://doi.org/10.1007/7651_2015_209