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Erschienen in:

2015 | Online First | Buchkapitel

Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Mononuclear Cells Using Sendai Virus

verfasst von : Filipa A. C. Soares, Roger A. Pedersen, Ludovic Vallier

Erschienen in: Methods in Molecular Biology™

Verlag: Springer New York

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Abstract

This protocol describes the efficient isolation of peripheral blood mononuclear cells from circulating blood via density gradient centrifugation and subsequent generation of integration-free human induced pluripotent stem cells. Peripheral blood mononuclear cells are cultured for 9 days to allow expansion of the erythroblast population. The erythroblasts are then used to derive human induced pluripotent stem cells using Sendai viral vectors, each expressing one of the four reprogramming factors Oct4, Sox2, Klf4, and c-Myc.
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Metadaten
Titel
Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Mononuclear Cells Using Sendai Virus
verfasst von
Filipa A. C. Soares
Roger A. Pedersen
Ludovic Vallier
Copyright-Jahr
2015
Verlag
Springer New York
DOI
https://doi.org/10.1007/7651_2015_202