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Erschienen in:

2015 | Online First | Buchkapitel

Generation of Integration-Free Patient Specific iPS Cells Using Episomal Plasmids Under Feeder Free Conditions

verfasst von : Sara Caxaria, Susanne Arthold, Amit C. Nathwani, Pollyanna Agnes Goh

Erschienen in: Methods in Molecular Biology™

Verlag: Springer New York

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Abstract

Reprogramming somatic cells into a pluripotent state involves the overexpression of transcription factors leading to a series of changes that end in the formation of induced pluripotent stem cells (iPSCs). These iPSCs have a wide range of potential uses from drug testing and in vitro disease modelling to personalized cell therapies for patients. While viral methods for reprogramming factor delivery have been traditionally preferred due to their high efficiency, it is now possible to generate iPSCs using nonviral methods at similar efficiencies. We developed a robust reprogramming strategy that combines episomal plasmids and the use of commercially available animal free reagents that can be easily adapted for the GMP manufacture of clinical grade cells.
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Metadaten
Titel
Generation of Integration-Free Patient Specific iPS Cells Using Episomal Plasmids Under Feeder Free Conditions
verfasst von
Sara Caxaria
Susanne Arthold
Amit C. Nathwani
Pollyanna Agnes Goh
Copyright-Jahr
2015
Verlag
Springer New York
DOI
https://doi.org/10.1007/7651_2015_204